A5035822233- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Vasculitis and related conditions
- Cell Adhesion Molecules Research
- Systemic Lupus Erythematosus Research
- Bioactive Compounds and Antitumor Agents
- Reproductive Biology and Fertility
- Pancreatic and Hepatic Oncology Research
- Genomics, phytochemicals, and oxidative stress
- Cytokine Signaling Pathways and Interactions
- Inflammasome and immune disorders
- Cholesterol and Lipid Metabolism
- ATP Synthase and ATPases Research
- Cancer, Hypoxia, and Metabolism
- Rheumatoid Arthritis Research and Therapies
- Immunodeficiency and Autoimmune Disorders
- Atherosclerosis and Cardiovascular Diseases
- Inflammatory Bowel Disease
- Systemic Sclerosis and Related Diseases
- Sexual Differentiation and Disorders
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Fatty Acid Research and Health
- Endoplasmic Reticulum Stress and Disease
- Galectins and Cancer Biology
Universidad de Granada
2015-2025
Instituto de Investigación Biosanitaria de Granada
2018-2025
Instituto de Parasitología y Biomedicina "López - Neyra"
2008-2011
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2011
Consejo Superior de Investigaciones Científicas
2008-2010
Parque Tecnológico de la Salud
2010
Corporación para Investigaciones Biológicas
2010
Universidad del Rosario
2010
Medizinische Hochschule Hannover
2010
Junta de Andalucía
2010
We determined the contribution of methylene tetrahydrofolate reductase (MTHFR) 677 C>T and 1298 A>C gene polymorphisms to susceptibility rheumatoid arthritis (RA). also assessed whether these two MTHFR may be implicated in development cardiovascular (CV) events subclinical atherosclerosis manifested by presence endothelial dysfunction, a series Spanish patients with RA. Six hundred twelve fulfilling 1987 American College Rheumatology classification criteria for RA, seen at rheumatology...
Pancreatic ductal adenocarcinoma is a deadly disease because of late diagnosis and chemoresistance. We aimed to find panel serum cytokines representing diagnostic predictive biomarkers for pancreatic cancer.A cytokine antibody array was performed simultaneously identify 507 in sera patients with cancer healthy controls. The nonparametric Mann-Whitney U test used pairwise compare the controls, pretreated patients, posttreated patients. Fold changes greater than or equal 1.5 less 1/1.5 were...
Abstract Protein dynamics is essential to understand protein function and stability, even though rarely investigated as the origin of loss-of-function due genetic variations. Here, we use biochemical, biophysical, cell computational biology tools study two cancer-associated polymorphisms (p.R139W p.P187S) in human NAD(P)H quinone oxidoreductase 1 (NQO1), a FAD-dependent enzyme which activates cancer pro-drugs stabilizes several oncosuppressors. We show that p.P187S strongly destabilizes NQO1...
Abstract STUDY QUESTION Do the genetic determinants of idiopathic severe spermatogenic failure (SPGF) differ between generations? SUMMARY ANSWER Our data support that component SPGF is impacted by dynamic changes in environmental exposures over decades. WHAT IS KNOWN ALREADY The form has a multifactorial etiology wherein an interaction genetic, epigenetic, and factors leads to disease onset progression. At level, genome-wide association studies (GWASs) allow analysis millions variants across...
OBJECTIVES: Genome-wide association studies have reported the role of interleukin (IL)2-IL21chromosomal region at 4q27 in several autoimmune conditions. Mice deficient IL-2 develop a disease with clinical and histological similarity to ulcerative colitis (UC) humans. Modest evidence linkage UC was tentatively proposed for theIL2gene more than decade ago. Therefore, we decide investigate polymorphisms axis (IL2,IL2RA,andIL2RBgenes) inflammatory bowel diseases (IBDs). METHODS: Seven hundred...
Interleukin-18 (IL-18) is a proinflammatory cytokine that plays an important role in chronic inflammation and autoimmune disorders. In this study, we aimed to determine the potential of IL18 gene SLE. To define genetic association SLE, have genotyped nine SNPs independent set Spanish cases controls. The polymorphisms were by PCR, using predeveloped TaqMan allele discrimination assay. Two still significant after fine mapping gene. SNP (rs360719) surviving correction for multiple tests was two...
Abstract Disease associated genetic variations often cause intracellular enzyme inactivation, dysregulation and instability. However, allosteric communication of mutational effects to distant functional sites leading loss-of-function remains poorly understood. We characterize here interdomain site-to-site by which a common cancer-associated single nucleotide polymorphism (c.C609T/p.P187S) reduces the activity stability in vivo NAD(P)H:quinone oxidoreductase 1 (NQO1). NQO1 is FAD-dependent,...
Human proteins are vulnerable towards disease-associated single amino acid replacements affecting protein stability and function. Interestingly, a few studies have shown that consensus acids from mammals or vertebrates can enhance when incorporated into human proteins. Here, we investigate yet unexplored relationships between the high vulnerability of inactivation recent evolutionary site-specific divergence stabilizing acids. Using phylogenetic, structural experimental analyses, show at...
The multifunctional nature of human flavoproteins is critically linked to their ability populate multiple conformational states. Ligand binding, post-translational modifications and disease-associated mutations can reshape this functional landscape, although the structure-function relationships these effects are not well understood. Herein, we characterized structural consequences two (the cancer-associated P187S phosphomimetic S82D) on different ligation states which relevant flavin...
Objective. Dendritic cells localized at the adventitia-media border of normal medium-sized arteries play a pivotal role in initiation giant cell arteritis (GCA). These express singular surface receptor profile, including series Toll-like receptors (TLR). Ligands TLR-4 promote activation and differentiation adventitial dendritic are directly implicated pathogenesis GCA. We aimed to assess potential implication TLR4-(+896 A/G) gene polymorphism susceptibility Methods. A total 210 patients...
Allosterism is a common phenomenon in protein biochemistry that allows rapid regulation of stability; dynamics and function. However, the mechanisms by which allosterism occurs (by mutations or post-translational modifications (PTMs)) may be complex, particularly due to long-range propagation perturbation across structures. In this work, we have investigated allosteric communication multifunctional, cancer-related antioxidant NQO1 mutating several fully buried leucine residues (L7, L10 L30)...
Abstract Introduction The objective was to investigate the potential implication of IL18 gene promoter polymorphisms in susceptibility giant-cell arteritis (GCA). Methods In total, 212 patients diagnosed with biopsy-proven GCA were included this study. DNA from and matched controls obtained peripheral blood. Samples genotyped for -137 G>C (rs187238), -607 C>A (rs1946518), -1297 T>C (rs360719) polymerase chain reaction, by using a predesigned TaqMan allele discrimination assay....
Objective. To examine the influence of STAT4 rs7574865 gene polymorphism on patients with primary Sjögren’s syndrome (SS). Methods. Two different cohorts were studied: 69 SS and 296 controls from Colombia 108 227 Germany. Samples genotyped for single-nucleotide a predesigned TaqMan genotyping assay. We carried out metaanalysis our results combined data published to date. Results. Although no significant differences observed in allele frequencies between Colombians (p = 0.28, OR 1.24, 95% CI...
The overall survival of patients with pancreatic ductal adenocarcinoma is extremely low. Although gemcitabine the standard used chemotherapy for this disease, clinical outcomes do not reflect significant improvements, even when combined adjuvant treatments. There an urgent need prognosis markers to be found. aim study was analyze potential value serum cytokines find a profile that can predict outcome in cancer and establish practical index significantly predicts patients' outcomes. We have...
Testes of seasonally breeding species experience a severe functional regression before the non-breeding period, which implies substantial mass reduction due to massive germ-cell depletion. Two alternative mechanisms seasonal depletion have been described in mammals, apoptosis and desquamation (sloughing), but their prevalence has not determined yet reduced number studied. We performed morphological, hormonal, molecular study mechanism testicular males Egyptian long eared-hedgehog...