A5100371452- MicroRNA in disease regulation
- Circular RNAs in diseases
- Wound Healing and Treatments
- Cancer-related molecular mechanisms research
- Catalytic C–H Functionalization Methods
- Radical Photochemical Reactions
- Sulfur-Based Synthesis Techniques
- Traumatic Brain Injury and Neurovascular Disturbances
- Histone Deacetylase Inhibitors Research
- Click Chemistry and Applications
- RNA Research and Splicing
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- Antimicrobial agents and applications
- Burn Injury Management and Outcomes
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- Amyotrophic Lateral Sclerosis Research
- RNA modifications and cancer
- S100 Proteins and Annexins
- Nanoplatforms for cancer theranostics
- Inflammasome and immune disorders
- Protein Degradation and Inhibitors
- Heme Oxygenase-1 and Carbon Monoxide
Nanjing Drum Tower Hospital
2022-2025
Guangdong Pharmaceutical University
2025
Nankai University
2024-2025
First Affiliated Hospital of Xinjiang Medical University
2025
Xinjiang Medical University
2025
Ocean University of China
2025
UCSF Helen Diller Family Comprehensive Cancer Center
2024
University of California, San Francisco
2018-2024
Xuzhou Medical College
2017-2024
East China University of Science and Technology
2024
Introducing new chemical reactivity into proteins in living cells would endow innovative covalent bonding ability to for research and engineering vivo. Latent bioreactive unnatural amino acids (Uaas) can be incorporated react with target natural acid residues via proximity-enabled reactivity. To expand the diversity of amenable such vivo, a functionality that is biocompatible able multiple under physiological conditions highly desirable. Here we report genetic encoding...
This study shows the effective use of MXene-based nanomaterials to improve performance biocomposite sponges in wound healing. In this way, diverse chitin/MXene composite are fabricated by incorporating with various morphology (accordion-shaped, intercalated, single-layer, gold nanoparticles (AuNPs)-loaded single-layer) into network chitin sponge (CH), which can prevent massive blood losses and promote healing process bacterial-infected wounds. With addition nanomaterials, hemostatic efficacy...
Posttraumatic neuroinflammation is a key driver of secondary injury after traumatic brain (TBI). Pyroptosis, proinflammatory form programmed cell death, considerably activates strong and amplifies the inflammatory response by releasing contents. Therefore, treatments targeting pyroptosis may have beneficial effects on treatment damage TBI. Here, cysteine-alanine-glutamine-lysine peptide-modified β-lactoglobulin (β-LG) nanoparticle was constructed to deliver disulfiram (DSF), C-β-LG/DSF,...
Metabolic reprogramming of cancer cells is essential for tumorigenesis in which pyruvate kinase M2 (PKM2), the low activity isoform kinase, plays a critical role. Herein, we describe identification nature-product-derived micheliolide (MCL) that selectively activates PKM2 through covalent binding at residue cysteine424 (C424), not contained PKM1. This interaction promotes more tetramer formation, inhibits lysine433 (K433) acetylation, and influences translocation into nucleus. In addition,...
Abstract Chronic wound management remains a challenge due to the epithelial barrier loss, drug‐resistant bacterial infections, inflammatory storm, and compromised angiogenesis. Herein, boron‐based probe driven theranostic hydrogel dressing for visual monitoring matching chronic wounds healing is developed encourage healing. The (BT) synthesized through dynamic interaction between borax (B) tannic acid (TA) serves as cross‐linker constructing guar gum/polyvinyl alcohol/BT (GPBT) hydrogels....
A new class of iminosulfonylation reagents were developed and extensively used in the 1,2-iminosulfonylation various olefins. Olefins containing bioactive molecules, such as indomethacin, gemfibrozil, clofibrate, fenbufen, afforded desired products synthetically useful yields. Furthermore, first remote 1,6-iminosulfonylation alkenes was realized by using oxime ester bifunctionalization reagents. Overall, more than 40 structurally diverse β-imine sulfones obtained moderate to excellent
Phosphorus fluoride exchange (PFEx), recently debuted in small molecules, represents the forefront of click chemistry. To explore PFEx's potential biological settings, we developed amino acids phosphoramidofluoridate tyrosine (PFY) and lysine (PFK) featuring phosphoramidofluoridates incorporated them into proteins through genetic code expansion. PFY/PFK selectively reacted with nearby His, Tyr, Lys, or Cys proteins, both vitro living cells, demonstrating that proximity enabled PFEx...
Ascl2, a basic helix-loop-helix transcription factor, is downstream target of WNT signaling that controls the fate intestinal cryptic stem cells and colon cancer progenitor cells. However, its involvement in molecular events largely undefined; particular, mechanism by which Ascl2 regulates plasticity epithelial-mesenchymal transition (EMT) mesenchymal-epithelial (MET) programs remains unknown. In this study, we systematically demonstrate loss function promotes MET derepressing expression...
Genetically introducing covalent bonds into proteins in vivo with residue specificity is affording innovative ways for protein research and engineering, yet latent bioreactive unnatural amino acids (Uaas) genetically encoded to date react one few natural residues only, limiting the variety of scope applications amenable this technology. Here we report genetic encoding (2R)-2-amino-3-fluoro-3-(4-((2-nitrobenzyl)oxy) phenyl) propanoic acid (FnbY) Escherichia coli mammalian cells. Upon...
Sialic acids are typically linked α(2-3) or α(2-6) to the galactose that located at non-reducing terminal end of glycans, playing important but distinct roles in a variety biological and pathological processes. However, details about their respective still largely unknown due lack an effective analytical technique. Herein, two-step chemoenzymatic approach for rapid sensitive detection N-acetylneuraminic acid-α(2-3)-galactose glycans is described.
Nowadays, simultaneous inhibition of multiple targets through drug combination is an important anticancer strategy owing to the complex mechanism behind tumorigenesis. Recent studies have demonstrated that histone deacetylases (HDACs) will lead compensated activation a notorious cancer-related target, signal transducer and activator transcription 3 (STAT3), in breast cancer cascade, which probably limits anti-proliferation effect HDAC inhibitors solid tumors. By incorporating pharmacophore...
Abstract As the most prevalent bone tumor in children and adolescents, pathogenesis metastasis of osteosarcoma (OS) remain largely unclear. Here, we investigated expression function a novel circular RNA (circRNA), circROCK1-E3/E4, which is back-spliced from exons 3 4 Rho-associated coiled-coil containing protein kinase 1 (ROCK1) OS. We found that regulated by well-known RNA-binding quaking (QKI), was downregulated OS correlated with unfavorable clinical features patients Functional...
Abstract Survival after severe traumatic brain injury (TBI) depends on minimizing or avoiding secondary insults to the brain. Overproduction of reactive oxygen species (ROS) and Ca 2+ influx at damaged site are key factors that cause upon TBI. Herein, a TBI‐targeted lipid covered radical scavenger nanoparticle is developed deliver nimodipine (Np) (CL‐PPS/Np), in order inhibit neurons by Np scavenge ROS trauma microenvironment poly(propylene sulfide) 60 (PPS ) thus prevent TBI‐associated...
A vinyl radical-mediated 1,5-hydrogen atom transfer strategy for remote C(sp3)-H imination under visible-light-induced photochemical metal-free conditions afforded diverse γ-imino alkenes with excellent stereoselectivity. Oxime ester-based bifunctional reagents provided not only nucleophilic alkyl radicals radical addition reactions electron-deficient alkynes but also long-lived steady-state imine trapping following the intramolecular migration of unstable olefin radicals.
In this study, a metal-free difunctionalization strategy for diazenes was developed using range of bifunctionalization reagents. This involves unique N(sp3)–N(sp2) radical coupling between the hydrazine and imine radical. More than 30 triazane core motifs were constructed by installing imines various functional groups, including alkyl, phenyl, cyanoalkyl, sulfonyl on both ends nitrogen–nitrogen bond in an efficient manner.
Axially chiral biaryl δ-amino acids possess significantly different conformational properties and environment from centrally amino acids, therefore, have drawn considerable attention in the fields of synthetic medicinal chemistry. Herein, a novel phenanthroline-potassium catalyst has been developed by constructing well-organized axially ligand composed one 1,10-phenanthroline unit two 1,1'-bi-2-naphthol (BINOL) units. In presence this catalyst, good to excellent yields enantioselectivities...
Circulating microRNAs (miRNAs) are emerging as novel disease biomarkers. Using a miRNA microarray, we previously showed that the whole blood level of let-7e-5p was significantly higher in ischemic stroke patients than control subjects. However, association between expression and occurrence remains unknown. In this study, validated levels two case-control populations using TaqMan assays further investigated potential targets let-7e-5p. The results suggest with controls (p<0.05). Higher were...