A5100321917- MicroRNA in disease regulation
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Aquaculture disease management and microbiota
- Immune Response and Inflammation
- Bacteriophages and microbial interactions
- Hypothalamic control of reproductive hormones
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Advanced biosensing and bioanalysis techniques
- Aquaculture Nutrition and Growth
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Sperm and Testicular Function
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Estrogen and related hormone effects
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Neural dynamics and brain function
- Immune cells in cancer
- Parkinson's Disease Mechanisms and Treatments
- Nitric Oxide and Endothelin Effects
- Regulation of Appetite and Obesity
First Affiliated Hospital of GuangXi Medical University
2025
Guangxi Medical University
2023-2025
Guangdong Academy of Medical Sciences
2023-2024
Southern Medical University
2013-2024
Guangdong Provincial People's Hospital
2023-2024
Beijing University of Technology
2024
Capital Medical University
2021-2024
Beijing Institute of Neurosurgery
2024
Sun Yat-sen University
2011-2024
Xuzhou Medical College
2015-2024
Aromatase (encoded by the cyp19a1a and cyp19a1b genes) plays a central role in sex differentiation fish, but its precise roles during are still largely unknown. Here, we systematically generated mutant lines as well cyp19a1a;cyp19a1b double line zebrafish using transcription activatorlike effector nucleases. Our results showed that mutants mutants, not had impaired differentiation, all were males. During ovary-like gonads observed male program was delayed cyp19a1a-null these phenotypes could...
Cardiac safety assessments are significant in drug discovery, as drug-induced cardiotoxicity (DIC) is the primary cause of attrition. Despite heart-on-a-chip (HoC) technology becoming an increasingly popular tool for evaluating DIC, its development remains a challenge owing to anisotropic cardiac structure native myocardium. Herein, multiscale scaffold presented via hybrid biofabrication method by combining 3D printing with electrospinning technology, where 3D-printed micrometer-scale frames...
Ascl2, a basic helix-loop-helix transcription factor, is downstream target of WNT signaling that controls the fate intestinal cryptic stem cells and colon cancer progenitor cells. However, its involvement in molecular events largely undefined; particular, mechanism by which Ascl2 regulates plasticity epithelial-mesenchymal transition (EMT) mesenchymal-epithelial (MET) programs remains unknown. In this study, we systematically demonstrate loss function promotes MET derepressing expression...
Spexin1 (SPX1) is a newly discovered neuropeptide in vertebrates. Its biological function remains to be elucidated. In this study, we have generated the zebrafish spx1 -/- mutant lines using transcription activator-like effector nucleases. Phenotypes of were analyzed order understand effects on reproduction and food intake. The reproductive capability not impaired zebrafish. However, found that fish had higher intake than wild type (WT) fish. Real-time PCR revealed expression level...
It is generally believed that kisspeptin and gonadotropin-releasing hormone (GnRH) are required for reproduction in vertebrates. In this study, we generated gnrh3-null zebrafish found gnrh3 mutation did not impair gonad development reproductive capacity. Moreover, triple knockout mutant lacking the 2 kiss1s genes undergo normal puberty maturation. The expression of follicle-stimulating beta (fshβ) luteinizing (lhβ) was significantly altered whereas neuropeptide Y (npy), tachykinin 3 (tac3),...
It is well established that the luteinizing hormone surge triggers ovulation, a dynamic process leading to release of mature oocyte from ovarian follicle. But how this controlled by LH signaling remains largely unknown in non-mammalian species. In study, we investigated roles nuclear progesterone receptor (npr) LH-induced ovulation. Our results indicate serves as an important mediator action on This conclusion based following results: (1) expression level npr peaks at full-grown stage...
Abstract Pre-surgery differential diagnosis is valuable for personalized treatment planning in intramedullary spinal cord tumors. This study assessed the performance of sequencing cell-free DNA (cfDNA) cerebrospinal fluid (CSF) these Prospectively enrolling 45 patients with lesions, including diffuse midline glioma (DMG), H3K27-altered (14/45), glioblastoma (1/45), H3-wildtype-astrocytoma (10/45), ependymoma (11/45), and other lesions (9/45), CSF samples were collected via lumbar puncture...
Neuregulins are highly expressed in the CNS, especially cholinergic neurons. We have examined effect of neuregulin on nicotinic acetylcholine receptors (nAChRs) neurons dissociated from rat hippocampus. Rapid application (ACh) induced a rapidly rising and decaying inward current some neurons, which was completely blocked by methyllycaconitine, specific antagonist α7 subunit nAChR. When cells were treated with 5 nm (NRG1-β1) for 2–4 d, twofold increase amplitude peak ACh-induced observed,...
To study the rapid action of estrogen on male reproductive system in teleost, a full-length cDNA homologous to seven-transmembrane receptor GPER humans and rodents was cloned from testis zebrafish. Biological characterization this zebrafish gper performed based its functional expression cultured eukaryotic cells. Saturation analysis Scatchard plotting [(3)H]-estradiol binding plasma membranes gper-transfected COS-7 cells cAMP response element transactivation assay demonstrated biological...
The pivotal role of androgen receptor (AR) in regulating male fertility has attracted much research attention the past two decades. Previous studies have shown that total AR knockout would lead to incomplete spermatogenesis and lowered serum testosterone levels mice, resulting azoospermia infertility. However, precise physiological ar controlling fish is still poorly understood. In this study, we established an zebrafish line by transcription activator-like effectors nucleases. Homozygous...