A5100630598- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Growth Hormone and Insulin-like Growth Factors
- Hypothalamic control of reproductive hormones
- Animal Genetics and Reproduction
- Regulation of Appetite and Obesity
- Immune Response and Inflammation
- Genomics and Phylogenetic Studies
- Sperm and Testicular Function
- Fish Biology and Ecology Studies
- Neuropeptides and Animal Physiology
- Identification and Quantification in Food
- Cancer, Hypoxia, and Metabolism
- Vibrio bacteria research studies
- Estrogen and related hormone effects
- interferon and immune responses
- Birth, Development, and Health
- Genetic and phenotypic traits in livestock
- Ferroptosis and cancer prognosis
- Ichthyology and Marine Biology
- Sleep and Wakefulness Research
- Cancer-related molecular mechanisms research
- Neuroendocrine regulation and behavior
Jiangsu Province Hospital
2020-2025
Nanjing Medical University
2020-2025
Hainan University
2014-2024
Qingdao National Laboratory for Marine Science and Technology
2019-2024
Jiangsu Normal University
2024
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2019-2024
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)
2021-2024
Guangdong Ocean University
2015-2024
North China University of Technology
2024
Pingtan Environmental Monitoring Center of Fujian
2023
Background Extensive research has established the significant correlations between cancer-associated fibroblasts (CAFs) and various stages of cancer development, including initiation, angiogenesis, progression, resistance to therapy. In this study, we aimed investigate characteristics CAFs in lung adenocarcinoma (LUAD) develop a risk signature predict prognosis patients with LUAD. Methods We obtained single-cell RNA sequencing (scRNA-seq) bulk RNA-seq data from public database. The Seurat R...
Background Glutamine metabolism (GM) is known to play a critical role in cancer development, including lung adenocarcinoma (LUAD), although the exact contribution of GM LUAD remains incompletely understood. In this study, we aimed discover new targets for treatment patients by using machine learning algorithms establish prognostic models based on GM-related genes (GMRGs). Methods We used AUCell and WGCNA algorithms, along with single-cell bulk RNA-seq data, identify most prominent GMRGs...
To ascertain the neuroendocrine function of kisspeptin/GPR54 system in non-mammalian species, full-length cDNAs encoding for Kiss1 and Kiss2 as well their putative cognate receptors GPR54a GPR54b, were isolated from goldfish ( Carassius auratus ). The deduced protein sequences between share very low similarity, but mature peptides (kisspeptin-10) are relatively conserved. RT-PCR analysis demonstrated that kiss1 gene gfkiss1 ) is highly expressed optic tectum-thalamus, intestine, kidney,...
The kiss1/gpr54 signaling system is considered to be a critical regulator of reproduction in most vertebrates. However, this presumption has not been tested vigorously nonmammalian Distinct from mammals, multiple paralogous genes (kiss/kissr) have identified vertebrates, raising the possibility functional redundancy among these genes. In study, we systematically generated zebrafish kiss1−/−, kiss2−/−, and kiss1−/−;kiss2−/− mutant lines as well kissr1−/−, kissr2−/−, kissr1−/−;kissr2−/− using...
Aromatase (encoded by the cyp19a1a and cyp19a1b genes) plays a central role in sex differentiation fish, but its precise roles during are still largely unknown. Here, we systematically generated mutant lines as well cyp19a1a;cyp19a1b double line zebrafish using transcription activatorlike effector nucleases. Our results showed that mutants mutants, not had impaired differentiation, all were males. During ovary-like gonads observed male program was delayed cyp19a1a-null these phenotypes could...
Background: Groupers (Epinephelus spp.) have been widely cultivated in China and South-East Asian countries.As a novel hybrid offspring crossed between E. fuscogutatus♀ lanceolatus♂, Hulong grouper exhibits significant growth superiority over its female parent, which made it promising farmed species aquaculture industry China.Hulong present good combination of beneficial traits from both parent species, but the molecular mechanisms heterosis still remain poorly understood.Results: Based on...
Background Current paradigms of anti-tumor therapies are not qualified to evacuate the malignancy ascribing cancer stroma’s functions in accelerating tumor relapse and therapeutic resistance. Cancer-associated fibroblasts (CAFs) has been identified significantly correlated with progression therapy Thus, we aimed probe into CAFs characteristics esophageal squamous (ESCC) construct a risk signature based on predict prognosis ESCC patients. Methods The GEO database provided single-cell RNA...
Background: Esophageal squamous cell carcinoma (ESCC) is a prevalent and aggressive form of cancer that poses significant challenges in terms prognosis treatment. Regulatory T cells (Treg cells) have gained attention due to their influential role immune modulation within the tumor microenvironment (TME). Understanding intricate interactions between Treg essential for unraveling mechanisms underlying ESCC progression developing effective prognostic models immunotherapeutic strategies....
Abstract To address the substantial variability in immune checkpoint blockade (ICB) therapy effectiveness, we developed an innovative R package called integrated Machine Learning and Genetic Algorithm‐driven Multiomics analysis (iMLGAM), which establishes a comprehensive scoring system for predicting treatment outcomes through advanced multi‐omics data integration. Our research demonstrates that iMLGAM scores exhibit superior predictive performance across independent cohorts, with lower...
The Kiss1/Kiss1r system is a component of the hypothalamus-pituitary-gonadal (HPG) axis, which plays crucial role in regulating gonadotropins and gonadotropin-releasing hormone. sex reversal process special reproductive phenomenon regulated by HPG axis. To better understand neuroendocrine mechanisms reversal, cDNAs encoding kiss2 kiss1ra have been cloned functionally characterized from orange-spotted grouper Epinephelus coioides, protogynous hermaphroditic teleost. core mature peptide...
Abstract This article documents the addition of 220 microsatellite marker loci to Molecular Ecology Resources Database. Loci were developed for following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco,...
The fish reproductive axis is regulated by many neuroendocrine factors. However, factors involved in the suppression of this are largely uncharacterized. In study, we describe a novel neuropeptide derived from spexin precursor acting as negative factor to suppress teleost. cDNA sequences precursors have been cloned both zebrafish and goldfish. A 14-aa mature peptide with C-terminal amidated (spexin-14a: NWTPQAMLYLKGTQ-NH2) conceivably generated processing species. Spexin mainly expressed...
Natural selection and selective breeding for genetic improvement have left detectable signatures within the genome of a species. Identification is important in evolutionary biology detecting genes that facilitate to accelerate improvement. However, signatures, including artificial natural selection, only been identified at whole level several genetically improved fish Tilapia one most species world. Using next-generation sequencing, we sequenced genomes 47 tilapia individuals. We total 1.43...
Spexin1 (SPX1) is a newly discovered neuropeptide in vertebrates. Its biological function remains to be elucidated. In this study, we have generated the zebrafish spx1 -/- mutant lines using transcription activator-like effector nucleases. Phenotypes of were analyzed order understand effects on reproduction and food intake. The reproductive capability not impaired zebrafish. However, found that fish had higher intake than wild type (WT) fish. Real-time PCR revealed expression level...
It is generally believed that kisspeptin and gonadotropin-releasing hormone (GnRH) are required for reproduction in vertebrates. In this study, we generated gnrh3-null zebrafish found gnrh3 mutation did not impair gonad development reproductive capacity. Moreover, triple knockout mutant lacking the 2 kiss1s genes undergo normal puberty maturation. The expression of follicle-stimulating beta (fshβ) luteinizing (lhβ) was significantly altered whereas neuropeptide Y (npy), tachykinin 3 (tac3),...
Background The Trachinotus ovatus (Teleostei, Carangidae) is an economically important marine fish species in the world. However, lack of genomic information regarding this limits our understanding genetics and biological mechanisms ovatus. In study, high throughput transcriptome sequencing was used to obtain comprehensive Principal Findings Transcriptome performed by using Illumina paired-end technology. 98,534,862 quality reads were yielded, de novo assembled into 156,094 unigenes with...
It is well established that the luteinizing hormone surge triggers ovulation, a dynamic process leading to release of mature oocyte from ovarian follicle. But how this controlled by LH signaling remains largely unknown in non-mammalian species. In study, we investigated roles nuclear progesterone receptor (npr) LH-induced ovulation. Our results indicate serves as an important mediator action on This conclusion based following results: (1) expression level npr peaks at full-grown stage...
Abstract The giant grouper ( Epinephelus lanceolatus ) is the largest coral reef teleost, with a native range that spans temperate and tropical waters in Pacific Indian Oceans. It cultured artificially used as breeding species aquaculture due to its rapid growth rate. Here we report genome assembled at chromosome scale from sequences generated using Illumina high‐throughput chromatin conformation capture (Hi‐C) technology. assembly comprised 1.086 Gb, 98.4% of scaffold anchored into 24...
Abstract The leopard coral grouper, Plectropomus leopardus , belonging to the family Epinephelinae, is a carnivorous reef fish widely distributed in tropical and subtropical waters of Indo‐Pacific. Due its appealing body appearance delicious taste, P. has become popular commercial for aquaculture many countries. However, lack genomic molecular resources hindered study biology breeding programmes. Here we report de novo sequencing assembly genome using combination 10 × Genomics,...
The continuous and extensive pinewood nematode disease has seriously threatened the sustainable development of forestry in China. At present, many studies have used high-resolution remote sensing images combined with a deep semantic segmentation algorithm to identify standing dead trees red attack period. However, due complex background, closely distributed detection scenes, unbalanced training samples, it is difficult detect (SDTs) variety scenes by using conventional models. In order...