A5034283397- Animal Genetics and Reproduction
- Aquaculture disease management and microbiota
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Reproductive biology and impacts on aquatic species
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Aquaculture Nutrition and Growth
- MicroRNA in disease regulation
- Retinal Development and Disorders
- Identification and Quantification in Food
- Invertebrate Immune Response Mechanisms
- Zebrafish Biomedical Research Applications
- Physiological and biochemical adaptations
- Reproductive Biology and Fertility
- Fish Ecology and Management Studies
- Virus-based gene therapy research
- Epigenetics and DNA Methylation
- Retinal Diseases and Treatments
- Hypothalamic control of reproductive hormones
- Cancer, Hypoxia, and Metabolism
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
University of Chinese Academy of Sciences
2021-2024
Institute of Hydrobiology
2012-2024
Chinese Academy of Sciences
2011-2024
Ocean University of China
2024
Huazhong Agricultural University
2021-2023
Key Laboratory of Guangdong Province
2023
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2022
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)
2022
State Key Laboratory of Freshwater Ecology and Biotechnology
2009-2022
Wuhan University
2011-2021
The kiss1/gpr54 signaling system is considered to be a critical regulator of reproduction in most vertebrates. However, this presumption has not been tested vigorously nonmammalian Distinct from mammals, multiple paralogous genes (kiss/kissr) have identified vertebrates, raising the possibility functional redundancy among these genes. In study, we systematically generated zebrafish kiss1−/−, kiss2−/−, and kiss1−/−;kiss2−/− mutant lines as well kissr1−/−, kissr2−/−, kissr1−/−;kissr2−/− using...
Abstract Farmed fish are a promising source of high‐quality and sustainable protein. However, the production farmed products that meet food safety standards is one major public health challenges worldwide. Therefore, management in farms has recently garnered increasing attention. detection parameters currently lacking. Haematology, an approach widely used humans, provides reliable assessment status non‐lethal low‐cost way. Nevertheless, comprehensive, systemic standard haematological have...
Prenatal lethality associated with mouse knockout of Mettl16, a recently identified RNA N6-methyladenosine (m
Abstract Mutations in EYS are associated with autosomal recessive retinitis pigmentosa (arRP) and cone-rod dystrophy (arCRD) however, the function of molecular mechanisms how these mutations cause retinal degeneration still unclear. Because is absent mouse rat, structure retina differs substantially between humans Drosophila , we utilised zebrafish as a model organism to study retina. We constructed an -knockout zebrafish-line by TALEN technology which showed visual impairment at early age,...
Abstract Prenatal dexamethasone exposure (PDE) induces multiorgan developmental toxicities in offspring. Here we verified the transgenerational inheritance effect of ovarian toxicity by PDE and explored its intrauterine programming mechanism. Pregnant rats subcutaneously received 0.2 mg/kg/d from gestational day (GD) 9 to GD20. A subgroup was euthanized for fetuses on GD20, other group went spontaneous labor produce F1 The adult females were mated with normal males F2 F3 generations. fetal...
This study aimed at analyzing the cytotoxicity and pathological effects of cadmium on ovary. Our studies revealed that was deposited in mouse ovary after 8 d injection vivo. Also, increase rate body weight slowed, while gonadosomatic index reduced CdCl2 group, compared with control group. Meanwhile, affected maturation follicles, degradation corpus luteum, arrangement follicles increased number atresia follicles. Besides, under electron microscope, chromatin margination, karopyknosis,...
Transcription activator-like effector nucleases (TALENs) have so far been applied to disrupt protein-coding genes which constitute only 2–3% of the genome in animals. The majority (70–90%) animal is actually transcribed as non-coding RNAs (ncRNAs), yet lack efficient tools knockout ncRNA hinders studies on their vivo functions. Here we developed novel strategies using TALENs achieve precise and inheritable large genomic deletions zebrafish. We demonstrated that individual miRNA could be...
Background The hypothalamic-pituitary-gonadal (HPG) axis is critical in the development and regulation of reproduction fish. inhibition neuropeptide gonadotropin-releasing hormone (GnRH) expression may diminish or severely hamper gonadal due to it being key regulator axis, then provide a model for comprehensive study patterns genes with respect fish reproductive system. Methodology/Principal Findings In previous we injected 342 fertilized eggs from common carp (Cyprinus carpio) gene...
Abstract Dysfunction of splicing factors often result in abnormal cell differentiation and apoptosis, especially neural tissues. Mutations pre-mRNAs processing factor 31 (PRPF31) cause autosomal dominant retinitis pigmentosa, a progressive retinal degeneration disease. The transcriptome-wide events specifically regulated by PRPF31 their biological roles the development maintenance retina are still unclear. Here, we showed that viability progenitor cells (RPCs) severely perturbed prpf31...
Abstract DMC1 is a recombinase that essential for meiotic synapsis. Experiments in extensive species of eukaryotes have indicated the independent role repairing double strand breaks (DSBs) produced during meiosis I. Mutation dmc1 mice and human often leads to obstacles spermatogenesis male sterility. Here, we report on disruption medaka ( Oryzias latipes ). Synapsis was disturbed mutant testis nuclei, as observed other organisms. Unexpectedly, could produce few sperm and, although most these...
Neural retina leucine zipper ( NRL ) is an essential gene for the fate determination and differentiation of precursor cells into rod photoreceptors in mammals. Mutations are associated with autosomal recessive enhanced S-cone syndrome dominant retinitis pigmentosa. However, exact role Nrl regulating development maintenance zebrafish Danio rerio ), a popular animal model used retinal degeneration regeneration studies, has not been fully determined. In this study, we generated nrl knockout via...
Prenatal caffeine exposure (PCE) can induce testicular developmental toxicity. Here, we aimed to explore the underlying mechanism of this process in reference its intrauterine origin. Pregnant rats were intragastrically administrated (30 and 120 mg/kg/day) from gestational days 9 20. The results showed that male fetuses exposed high dose (120 had a decreased bodyweight inhibited testosterone synthetic function. Meanwhile, their serum corticosterone concentration was elevated insulin-like...
Somatic cell nuclear transfer (SCNT) has been performed extensively in fish since the 1960s with a generally low efficiency of approximately 1%.Little is known about somatic reprogramming fish.Here, we utilized zebrafish as model to study events nuclei from tail, liver and kidney cells by SCNT.We produced total 4,796 reconstituted embryos obtained high survival rate 58.9-67.4% initially at 8-cell stage.The exhibited two steps dramatic decrease, leading 8.7-13.9% dome stage 1.5-2.96%by shield...