A5100321938- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- MicroRNA in disease regulation
- RNA Research and Splicing
- Uterine Myomas and Treatments
- Endometrial and Cervical Cancer Treatments
- Cancer-related gene regulation
- Ear Surgery and Otitis Media
- Dermatologic Treatments and Research
- Congenital Diaphragmatic Hernia Studies
- Ear and Head Tumors
- Tracheal and airway disorders
- Hearing, Cochlea, Tinnitus, Genetics
- Cancer Immunotherapy and Biomarkers
- Omental and Epiploic Conditions
- Healthcare Systems and Public Health
- Metabolism, Diabetes, and Cancer
- Sarcoma Diagnosis and Treatment
- Mycobacterium research and diagnosis
- Neuroblastoma Research and Treatments
- Traditional Chinese Medicine Analysis
- Healthcare Systems and Reforms
- Plant and Fungal Interactions Research
- Endometriosis Research and Treatment
Nanjing Medical University
2017-2025
Jiangsu Province Hospital
2019-2025
Beijing Friendship Hospital
2015-2024
Capital Medical University
2010-2024
Tianjin Medical University Cancer Institute and Hospital
2017-2023
National Clinical Research
2017-2023
Tianjin Medical University
2017-2023
First Affiliated Hospital of GuangXi Medical University
2022-2023
Guangxi Medical University
2022-2023
Huazhong University of Science and Technology
2019-2023
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a broadly expressed lncRNA involved in many aspects of cellular processes. To further delineate the underlying molecular mechanism, we employed high-throughput strategy to characterize interacting proteins MALAT1 by combining RNA pull-down, quantitative proteomics, bioinformatics, and experimental validation. Our approach identified 127 potential MALAT1-interacting established highly connected interactome network consisting...
Abstract NEAT1 is an important tumor oncogenic gene in various tumors. Nevertheless, its involvement remains poorly studied cervical cancer. Our study explored the functional mechanism of level several cancer cells was quantified and we found greatly upregulated vitro. knockdown inhibited development through repressing cell proliferation, colony formation, capacity migration, invasion also inducing apoptosis. For another, microRNA (miR)‐133a downregulated negatively modulated miR‐133a...
Breast cancer was dangerous to women health. A growing number of evidences indicate that long non-coding RNAs (lncRNAs) have many functions in the development and progression breast may serve as markers diagnosis or prognosis. BLACAT1 is one lncRNA roles it not clear. In this study, aimed explore molecular mechanisms cancer.We found took part cell aggressive phenotype. The real-time PCR result showed up-regulated tumor tissues compared adjacent normal tissues. mechanism experiments...
Extrachromosomal circular DNA (eccDNA) has emerged as a potential biomarker for disease due to its stable closed structure. However, the diagnostic utility of eccDNA remains underexplored. In this study, we demonstrate that characteristics associated with genomic repetitive elements change in breast cancer patient tissues and plasma. These changes can serve signatures accurate classification. We profiled annotated repeat across genome plasma, aggregating each element superfamily subfamily...
Transcription activator-like effector nucleases (TALENs) have so far been applied to disrupt protein-coding genes which constitute only 2–3% of the genome in animals. The majority (70–90%) animal is actually transcribed as non-coding RNAs (ncRNAs), yet lack efficient tools knockout ncRNA hinders studies on their vivo functions. Here we developed novel strategies using TALENs achieve precise and inheritable large genomic deletions zebrafish. We demonstrated that individual miRNA could be...
Accumulating research works have reported that long noncoding RNAs (lncRNAs) are involved in various cancers, including cervical cancer. LncRNA DGCR5 has been identified many cancers. However, the biological role of cancer remains barely known. We aimed to investigate function progression. Here, our current study, we observed was downregulated human cell lines (MS751, SiHa, HeLa, and HT-3) compared with primary normal squamous cells (NCSC1 NCSC2). Then, restrained by transfection...
Long non-coding RNA (lncRNA) plays important roles in many diseases, including pulmonary hypertension. The anti-diabetic drug metformin has been discovered to have protective effect on experimental artery hypertension (PAH). However, the exact mechanism of expression lncRNA PAH remains unclear. Here, we applied microarray analysis examine and mRNA proflies arteries (PAs) tissues rats with or without treatment. A total 24 lncRNAs (14 upregulated 10 down-regulated) 82 mRNAs (17 65 were...
Endometrial carcinoma (EC) is one of the major gynecologic malignancy cancers affecting females with dismal prognosis and high mortality around world. Numerous studies have proven that an aberrant level long noncoding RNAs present in many endometrial cancer patients, while underlying molecular mechanism remains unclear. The expression levels lncRNA OIP5-AS1, miR200c-3p, PTEN were measured by a quantitative real-time polymerase chain reaction tissue cells. CCK8 assay, wound-healing cell...
Neuroblastomas have shed light on the differentiation disorder that is associated with spontaneous regression or in same tumor at time. Long noncoding RNAs (lncRNAs) actively participate a broad spectrum of biological processes. However, detailed molecular mechanisms underlying lncRNA regulation neuroblastomas remain largely unknown. Here, we sequenced clinical samples ganglioneuroma, ganglioneuroblastoma, and neuroblastoma. We compared transcription profiles neuroblastoma cells, ganglion...
Background: Breast cancer (BC) is a highly heterogeneous cancer. The interaction between immune system and BC complex, widespread yet unclear. In this study, we aimed to reveal the heterogeneity of host systemic response understand possible mechanisms that may drive using transcriptomic data from peripheral blood mononuclear cells (PBMCs). Methods: Transcriptome-wide gene expressions PBMCs in 33 patients were generated by RNA sequencing. An unsupervised clustering algorithm was employed...
Osteosarcoma (OS) is a primary bone tumor with high malignancy and the mechanism of hematogenous metastasis in OS still not clear. The plasma exosomes derived from osteosarcoma play key role process metastasis. Here, we established RNA-seq dataset for lncRNAs, circRNAs mRNAs 10 patients 5 healthy donors. A total 329.52 Gb clean data was obtained. Besides, 1754 lincRNAs, 7096 known 1935 new circRNA identified. Finally, gene expression profiles differentially expressed genes (DEGs) were...
Endometrial cancer is a heterogeneous disease with distinct molecular characteristics, however, the current clinical trials in immunotherapies have reported only 13% response rate endometrial cancer. In this study, we aim to estimate relative abundance of immune cells infiltrating into tumor tissues. The samples were clustered based on cell abundance. Most cluster-specifically mutated genes detected clusters I and II, while copy number alterations specifically cluster III. Overrepresentation...
Aberrantly expressed lncRNAs have been reported to be closely related the oncogenesis and development of osteosarcoma. However, role a dysregulated lncRNA-miRNA-mRNA network in osteosarcoma same individual needs further investigated. Whole transcriptome sequencing was performed on tumour tissues matched paratumour three patients with confirmed Two divergent regulatory networks were constructed accordance their biological significance. The GO KEGG analysis results mRNAs two revealed that...
Leiomyomatosis peritonealis disseminata (LPD) is a rare benign lesion primarily consisting of smooth muscle cells, which mostly affects premenopausal females. Here, we reported 3 females with LPD (age, 40-48 years) admitted for pelvic masses.All cases received laparoscopic uterine fibroid morcellation at 3, 8, and 14 years ago, respectively. Two were masses. One case was recurrent fibroids pollakiuria.LPD considered in 2 preoperation according to imaging examination, one them...
Long non-coding RNAs (lncRNAs) have been demonstrated to regulate metabolic tissue development and function, including adipogenesis, hepatic lipid metabolism, islet function energy balance. However, the role of lncRNAs in gluconeogenesis remains completely unknown. Metformin reduces glucose output mainly via inhibition gluconeogenesis. In present study, lncRNA expression profile primary mouse hepatocytes exposed cyclic adenosine monophosphate (cAMP), a gluconeogenic stimulus, with or without...
Background: Accurate preoperative diagnosis of endometrial cancer (EC) with deep myometrial invasion (DMI) is critical to deciding whether perform lymphadenectomy. However, the presence adenomyosis makes distinguishing DMI from superficial (SMI) on magnetic resonance imaging (MRI) challenging. We aimed evaluate accuracy multiparametric MRI (mpMRI) in diagnosing EC coexisting (EC-A) compared without and effect different subtypes (MI) depth EC. Methods: Patients histologically confirmed...
// Fan 1, 2 , Zhengqiu Zhu 3 Chao Gao Yun Liu 1 Baoqing Wang Ziquan and Jifeng Feng Department of Chemotherapy, Nanjing Medical University Affiliated Cancer Hospital, Jiangsu Institute Research, 210009, China The No.2 Hospital Xuzhou University, 221000, Correspondence to: Feng, email: medfjif@sina.com Keywords: lncRNA, lung cancer, prognosis Received: July 06, 2017 Accepted: August 29, Published: September 20, ABSTRACT Many different long non-coding RNAs (lncRNAs) have been reported to be...