A5100613706- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- TGF-β signaling in diseases
- Phytochemistry and Biological Activities
- Epigenetics and DNA Methylation
- Renal and related cancers
- Pluripotent Stem Cells Research
- Plant-based Medicinal Research
- Aquaculture disease management and microbiota
- CRISPR and Genetic Engineering
- Protease and Inhibitor Mechanisms
- Phytochemical Studies and Bioactivities
- FOXO transcription factor regulation
- Signaling Pathways in Disease
- Mosquito-borne diseases and control
- Reproductive Biology and Fertility
- Endoplasmic Reticulum Stress and Disease
- Peptidase Inhibition and Analysis
- Congenital heart defects research
- Glycosylation and Glycoproteins Research
Tianjin Medical University Cancer Institute and Hospital
2023-2025
National Clinical Research
2025
Sichuan University
2024
West China Hospital of Sichuan University
2024
First Hospital of Jilin University
2024
Jilin University
2024
Nanjing Agricultural University
2014-2022
The First Affiliated Hospital, Sun Yat-sen University
2022
Sun Yat-sen University
2022
Ministry of Education of the People's Republic of China
2022
We conducted genome-wide miRNA-sequencing (miRNA-seq) in primary cancer tissue from patients of lung adenocarcinoma to identify markers for the presence lymph node metastasis.Markers metastasis identified by sequencing were validated a separate cohort using quantitative PCR. After additional validation The Cancer Genome Atlas (TCGA) dataset, functional characterization studies vitro.MiR-31 was upregulated tissues with metastases compared those without metastases. confirmed miR-31 be...
Recent studies reported the important role of autophagy in follicular development. However, underlying molecular mechanisms remain elusive. In this study, we investigated effect follicle-stimulating hormone (FSH) on mouse granulosa cells (MGCs). Results indicated that was induced by FSH, which is known to be dominant regulating development and cell (GC) proliferation. The activation mammalian target rapamycin (mTOR), a master regulator autophagy, inhibited during process MGC autophagy....
In mammals, more than 99% of ovarian follicles undergo a degenerative process known as atresia. The molecular events involved in atresia initiation remain incompletely understood. objective this study was to analyze differential gene expression profiles medium antral during early pig. transcriptome evaluation performed on cDNA microarrays using healthy and atretic follicle samples validated by quantitative PCR. Annotation analysis applying current database (Sus scrofa 11.1) revealed 450...
Duck Tembusu virus (DTMUV) can cause serious disease in ducks, characterized by reduced egg production. Although the has been isolated and detection methods developed, host immune responses to DTMUV infection are unclear. Therefore, we systematically examined expression of immune-related genes viral distribution DTMUV-infected using quantitative real-time PCR. Our results show that replicates quickly many tissues early infection, with highest titers spleen 1 day after infection. Rig-1, Mda5,...
Phosphodiesterase 4 (PDE4) inhibitors are effective therapeutic agents for various inflammatory diseases. Roflumilast, apremilast, and crisaborole have been developed approved the treatment of chronic obstructive pulmonary disease psoriatic arthritis, atopic dermatitis. Inflammation underlies many vascular diseases, yet role PDE4 in these diseases remains inadequately explored. This review elucidates clinical applications anti-inflammatory mechanisms inhibitors, as well their potential...
Smad7 has a key role in apoptosis of mammalian ovarian granulosa cells (GCs), as it antagonizes and fine‐tunes transforming growth factor β (TGFβ) signaling. This study demonstrates that miR‐92a regulates GC pig ovaries by targeting directly. The expression level was down‐regulated atretic porcine follicles, whereas led to inhibition apoptosis. gene identified direct target using dual‐luciferase reporter assay. Transfection GCs with mimics decreased mRNA protein levels, an inhibitor had the...
Abstract The hyaluronan synthase 2 (HAS2)-hyaluronic acid (HA)-CD44-Caspase-3 pathway is involved in ovarian granulosa cell (GC) functions mammals. HAS2 a key enzyme required for HA synthesis and the factor this pathway. However, regulation of HAS2-mediated by microRNAs GCs poorly understood. Here, we report that miR-26b regulates porcine GC (pGC) apoptosis through HAS2-HA-CD44-Caspase-3 binding directly to 3′- untranslated region mRNA. Knockdown reduced pGC apoptosis. Luciferase reporter...
In developing follicles, the granulosa cells (GCs) live in a hypoxic environment due to devoid of blood supply. Upon conditions, several types mammalian have been reported undergo apoptosis. Follicle-stimulating hormone (FSH) is known as primary survival factor for antral follicles by preventing GCs Mitophagy type organelle-specific autophagy that removes damaged or stressed mitochondria maintain cellular health. This study provides first evidence suggesting FSH-mediated mitophagy protected...
Percutaneous local thermal therapy, containing radio frequency and microwave ablation, is widely utilized in the clinical management of hepatocellular carcinoma (HCC) due to its minimal invasiveness favorable therapeutic outcomes. However, further development limited by a prolonged ablation duration risk excessive heating. Magnetic hyperthermia therapy (MHT) provides new perspective for percutaneous tumor superior tissue penetration capability safety. Herein, an iron foam-agarose gel-drug...
The urokinase-type plasminogen activator receptor (u-PAR) facilitates extracellular matrix proteolysis by accelerating plasmin formation at the cell surface. present study was undertaken to identify elements in u-PAR promoter required for elevated expression of this binding site. Toward end, we used two cultured colon cancer lines; one (RKO) has a transcriptionally activated <i>u-PAR</i> gene, and other (GEO) overexpresses only after phorbol ester treatment. A chloramphenicol...
Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components. Here we showed autophagy was for the glycolysis switch energy homeostasis in mouse granulosa cells under hypoxic condition. Our data indicated hypoxia inducible factor-1α (HIF-1α) could be largely increased developing follicles this remarkable upregulation of HIF-1α triggered cell glucose uptake. We found blocking by Atg7 knockdown 3-methyladenine (3-MA) treatment...
SMAD7 disrupts the TGF‐β signaling pathway by influencing TGFBR1 stability and blocking binding of to SMAD2/3. In this study, we showed that attenuated in ovarian granulosa cells (GCs) regulating transcriptional activity. To function as a transcription factor, downregulated mRNA levels via direct SMAD‐binding elements (SBEs) within promoter region pig TGFBR1. We also enhanced porcine GC apoptosis interrupting pathway. Interestingly, miR‐181b, microRNA is during follicular atresia, was...
Abstract Follicle-stimulating hormone (FSH)-induced growth of ovarian follicles is independent follicular vascularization. Recent evidence has indicated that vascularization critical to follicle development and survival. FSH, a gonadotropin induces development, also acts as the major survival factor for antral follicles. FSH been reported stimulate angiogenesis in theca layers mediated part by vascular endothelial A (VEGFA) transcription hypoxia inducible 1α (HIF-1α). However, it remains...
Betaglycan is a type III TGFbeta receptor that modulates cellular sensitivity to inhibins and TGFbeta. Previous studies have suggested betaglycan acts as tumor suppressor in certain human epithelial cancers. However, the roles of ovarian granulosa cell tumors (GCTs) are poorly understood. The objective this study was determine whether GCTs exhibit expression and, if so, what impact has on biology. Real-time PCR used quantify transcripts (n = 17) normal premenopausal ovaries 11). This...