A5059609832- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Meat and Animal Product Quality
- Adipose Tissue and Metabolism
- Genetic and phenotypic traits in livestock
- RNA Research and Splicing
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Genetic Mapping and Diversity in Plants and Animals
- Circular RNAs in diseases
- Muscle Physiology and Disorders
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- FOXO transcription factor regulation
- Electromagnetic wave absorption materials
- Autophagy in Disease and Therapy
- Mesenchymal stem cell research
- Molecular Biology Techniques and Applications
- Cell death mechanisms and regulation
- Animal Nutrition and Physiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Lipid metabolism and biosynthesis
Nanjing Agricultural University
2016-2025
Zhejiang Academy of Forestry
2022-2024
Zhejiang A & F University
2022-2024
Huazhong Agricultural University
2011-2014
Zero to Three
2014
Ministry of Education of the People's Republic of China
2010-2011
Abstract The gut microbiota plays an important role in nutrient digestibility animals. To examine changes the pig across growth stages and its effects on digestion, population pigs at 28 days (before weaning) 60, 90 150 of age was assessed by 16S rDNA gene sequencing. apparent crude fiber (CF), neutral detergent (NDF), acid (ADF), protein (CP) ether extract (EE) also these pigs. A total 19,875 operational taxonomic units (OTUs) were identified from all samples. Both bacterial abundance...
Individual skeletal muscles in the animal body are heterogeneous, as each is comprised of different fiber types.Type I muscle fibers rich with mitochondria, and have high oxidative metabolisms while type IIB few mitochondria glycolytic metabolic capacity.Peroxisome proliferator-activated receptor gamma coactivator 1α (PGC-1α), a transcriptional co-activator that regulates mitochondrial biogenesis respiratory function, implicated fiber-type switching.Over-expression PGC-1α transgenic mice...
Recent studies reported the important role of autophagy in follicular development. However, underlying molecular mechanisms remain elusive. In this study, we investigated effect follicle-stimulating hormone (FSH) on mouse granulosa cells (MGCs). Results indicated that was induced by FSH, which is known to be dominant regulating development and cell (GC) proliferation. The activation mammalian target rapamycin (mTOR), a master regulator autophagy, inhibited during process MGC autophagy....
The c-Jun N-terminal protein kinase (JNK) plays an important role in the regulation of cell apoptosis. Forkhead box O (FoxO) transcription factors are involved diverse biological processes, including cellular metabolism, apoptosis, and cycle. However, JNK/FoxO1 pathway process apoptosis induced by oxidative stress remains to be elucidated. Here, we demonstrated that JNK activity significantly increased response mouse follicular granulosa cells (MGCs). SP600125, a selective inhibitor,...
Intramuscular fat (IMF) content is an important indicator for meat quality evaluation. However, the key genes and molecular regulatory mechanisms affecting IMF deposition remain unclear. In present study, we identified 75 differentially expressed (DEGs) between higher (H) lower (L) of pigs using transcriptome analysis, which 27 were upregulated 48 downregulated. Notably, Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis indicated that DEG perilipin-1 (PLIN1) was significantly...
In obesity and diabetes, intramuscular fat (IMF) content correlates markedly with insulin sensitivity, which makes IMF manipulation an area of therapeutic interest. Melatonin, important circadian rhythm-regulating hormone, reportedly regulates deposition, but its effects on different types adipose vary. Little is known about the role melatonin in deposition. Here, using preadipocytes pigs, we investigated to determine whether affects or We found that greatly inhibited porcine preadipocyte...
Intramuscular fat (IMF) content plays an important role in pork quality. Circular RNAs (circRNAs) implicate various biological processes; however, the regulatory mechanisms and functions of circRNAs porcine IMF remains elusive. Hence, study assessed circRNA expression profiling longissimus dorsi muscle pigs with high (H) low (L) to unravel their improving meat The RNA sequencing analysis identified 29,732 from six sampled pigs, most which were exon-derived. In muscle, 336 differentially...
Excellent electromagnetic wave absorption ability and effective bandwidth have been the development direction of absorbing materials. In this paper, a rational structural design is proposed. Graphene oxide (GO)/polyaniline (PANI) layer was formed on surface natural material bamboo powder (BP) by an interfacial polymerization process. The combination GO PANI improves impedance mismatch conductive polymer PANI. Enhanced dielectric storage loss capability composite GO/PANI/BP. addition BP...
<title>Abstract</title> Pork color is one of the most important attributeswhich reflect meat quality and affect consumer perception purchase decisions.However, rapid objectivepork scoring method still lack. The traditional methodsfor construction pork models depend on a large sample sizeand face problem imbalance,and slope intercept calibration significantly rely source samples device.To addressed limitations,a for constructing precision based six standard boardimages was proposed in this...
Fibroblast growth factor 21 (FGF21) plays an important role in the treatment of disease associated with muscle insulin resistance which is characterized by various factors, such as intramuscular triglyceride (IMT) content. Studies have also shown that FGF21 inhibits synthesis vivo. However, precise mechanism whereby regulates metabolism fat (IMF), may influence sensitivity, not clearly understood. In order to understand IMF deposition, we performed overexpression cells stable transfection....
Abstract Meat color is considered to be the most important indicator of meat quality, however, molecular mechanisms underlying traits related remain mostly unknown. In this study, elucidate basis color, we constructed six cDNA libraries from biceps femoris (Bf) and soleus (Sol), which exhibit obvious differences in analyzed whole-transcriptome between Bf (white muscle) Sol (red using high-throughput sequencing technology. Using DEseq2 method, identified 138 differentially expressed genes...
The objective of this study was to establish the optimum protocol for isolation and culture porcine muscle satellite cells. Mononuclear cells are a kind adult stem cell, which is located between basal lamina sarcolemma fibers primary source myogenic precursor in postnatal muscle. Muscle useful model investigate mechanisms growth development. Although protocols some species (e.g. mouse) have been established successfully, system very limited. In study, we optimized procedure elaborated...
The alteration in skeletal muscle fiber is a critical factor affecting livestock meat quality traits and human metabolic diseases. Long non-coding RNAs (lncRNAs) are diverse class of with length more than 200 nucleotides. However, the mechanisms underlying regulation lncRNAs fibers remain elusive. To understand genetic basis lncRNA-regulated development, we performed transcriptome analysis to identify key on pig model. We generated lncRNA expression profiles fast-twitch Biceps femoris (Bf)...
Low cloning efficiency is considered to be caused by the incomplete or aberrant epigenetic reprogramming of differentiated donor cells in somatic cell nuclear transfer (SCNT) embryos. Oxamflatin, a novel class histone deacetylase inhibitor (HDACi), has been found improve vitro and full-term developmental potential SCNT In present study, we studied effects oxamflatin treatment on porcine Our results indicated that rate blastocyst formation embryos treated with 1 μM for 15 h postactivation was...
Oxidative stress (OS) plays an important role in the process of ovarian granulosa cell apoptosis and follicular atresia. The aim this study was to select antioxidant against OS ovary tissue. Firstly, we chose six antioxidants analyzed reactive oxygen species (ROS) level results showed that proanthocyanidins, gallic acid, curcumin, carotene decrease ROS compared with control group. We further demonstrated both proanthocyanidins acid increase enzymes activity. Moreover, change not observed...
The different skeletal muscle fiber types exhibit distinctively physiological and metabolic properties, have been linked to both human diseases meat quality traits in livestock. Circular RNAs (circRNAs) are a new class of endogenous RNA regulating gene expression, but regulatory mechanisms fibers involved circular remain poorly understood. Here, we constructed circRNA expression profiles three fast-twitch biceps femoris (Bf) slow-twitch soleus (Sol) muscles pigs using RNA-seq identified...
Skeletal muscle is a major component of body mass and plays central role in the control whole‐body metabolism humans animals. Therefore, elucidation underlying mechanisms skeletal growth development are expected to lead discovery novel genes pathways related disease. miR‐206, muscle‐specific microRNA, crucial myogenesis; however, miR‐206 known function myogenic differentiation, whether or not it affects cells' proliferation, unknown. In this study, we investigated effect on cell...
The Meishan pig is a traditional Chinese native breed, known for its excellent reproduction performance that widely used in commercial production through two-way or three-way crossbreeding systems. However, the lean meat yield of crossbred pigs still very low and cannot meet market demand. To evaluate pigs, six wild-type sows were artificially inseminated by using MSTN+/- Duroc boar semen this experiment. Some reproductive performance-related traits recorded to ensure from MSTN knockout did...