A5045883594- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- TGF-β signaling in diseases
- Kruppel-like factors research
- Microtubule and mitosis dynamics
- Pancreatic and Hepatic Oncology Research
- Immune cells in cancer
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Endometriosis Research and Treatment
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Reproductive System and Pregnancy
- Cancer, Lipids, and Metabolism
- Cellular Mechanics and Interactions
- Trace Elements in Health
- Gynecological conditions and treatments
- Cancer, Hypoxia, and Metabolism
- Liver physiology and pathology
- Protease and Inhibitor Mechanisms
- Fibroblast Growth Factor Research
- Pregnancy-related medical research
- Cell Adhesion Molecules Research
- Single-cell and spatial transcriptomics
- Lung Cancer Research Studies
Hudson Institute of Medical Research
2014-2024
Monash University
2013-2024
Monash Health
2021
Deakin University
2013
St Vincents Institute of Medical Research
2013
Monash Institute of Medical Research
2009
Tumor cells in ascites are a major source of disease recurrence ovarian cancer patients. In an attempt to identify and profile the population obtained from patients, novel method was developed separate adherent (AD) non-adherent (NAD) culture. Twenty-five patients were recruited this study; 11 chemonaive (CN) 14 chemoresistant (CR). AD both CN CR exhibited mesenchymal morphology with antigen stem fibroblasts. Conversely, NAD had epithelial enhanced expression 125 (CA125), cell adhesion...
The therapeutic efficacy of two bis(thiosemicarbazonato) copper complexes, glyoxalbis[N4-methylthiosemicarbazonato]CuII [CuII(gtsm)] and diacetylbis[N4-methylthiosemicarbazonato]CuII [CuII(atsm)], for the treatment prostate cancer was assessed in cell culture animal models. Distinctively, dissociates intracellularly from CuII(gtsm) but is retained by CuII(atsm). We further demonstrated that intracellular H2gtsm [reduced CuII(gtsm)] continues to redistribute into a bioavailable (exchangeable)...
Epithelial ovarian cancer metastasis is driven by spheroids, which are heterogeneous cell aggregates released from the primary tumour mass that passively disseminate throughout peritoneal cavity to promote spread, disease recurrence, and acquired chemoresistance. Despite their clinical importance, molecular events control spheroid attachment invasion into underlying healthy tissues remain poorly understood. We examined a novel in vitro model using imaging spectrometry establish "snapshot" of...
For the vast majority of ovarian cancer patients, optimal surgical debulking remains a key prognostic factor associated with improved survival. A standardized, biomarker-based test, to preoperatively discriminate benign from malignant disease and inform appropriate patient triage, is highly desirable. However, no fit-for-purpose biomarkers have yet been identified.We conducted pilot study consisting 40 urine samples (20 each group), using label-free quantitative (LFQ) mass spectrometry,...
STUDY QUESTIONDo human blastocysts which subsequently implant release factors that regulate endometrial epithelial cell gene expression and adhesion to facilitate receptivity?
Abstract Background The metzincin family of metalloproteinases and the tissue inhibitors (TIMPs) are essential proteins required for biological processes during cancer progression. This study aimed to determine role TIMP-2 in ovarian progression chemoresistance by reducing expression vitro Fallopian tube secretory epithelial (FT282) (JHOS2 OVCAR4) cell lines. Methods FT282, JHOS2 OVCAR4 cells were transiently transfected with either single or pooled siRNAs. different genes after knock down...
Betaglycan is a type III TGFbeta receptor that modulates cellular sensitivity to inhibins and TGFbeta. Previous studies have suggested betaglycan acts as tumor suppressor in certain human epithelial cancers. However, the roles of ovarian granulosa cell tumors (GCTs) are poorly understood. The objective this study was determine whether GCTs exhibit expression and, if so, what impact has on biology. Real-time PCR used quantify transcripts (n = 17) normal premenopausal ovaries 11). This...
Immunity plays a key role in epithelial ovarian cancer (EOC) progression with well-documented correlation between patient survival and high intratumoral CD8+ to T regulatory cell (Treg) ratios. We previously identified dysregulated DPP4 activity EOCs as potentially immune-disruptive influence contributing reduction CXCR3-mediated T-cell infiltration solid tumours. therefore hypothesized that inhibition of by sitagliptin, an FDA-approved inhibitor, would improve function syngeneic ID8 mouse...
Abstract The molecular pathways controlling granulosa cell tumor (GCT) survival are poorly understood. In many types, nuclear factor-κB (NFκB) and TGFβ coordinately regulate to maintain tissue homeostasis. Because GCT lines exhibit constitutively activated NFκB, we hypothesized that NFκB blocks TGFβ-mediated death in cells. To test this hypothesis, used the human line KGN, which exhibits loss of betaglycan, a co-receptor. After inhibition KGN cells, re-expression betaglycan resulted decrease...
Abstract Oct4A is a master regulator of self-renewal and pluripotency in embryonic stem cells. It well-established marker for cancer cell (CSC) malignancies. Recently, using loss function studies, we have demonstrated key roles tumor survival, metastasis chemoresistance vitro vivo models ovarian cancer. In an effort to understand the regulatory role biology, employed use shRNA knockdown line (HEY KD) global mass spectrometry (MS)-based proteomic analysis investigate novel biological targets...
The treatment of ovarian cancer has not significantly changed in decades and it remains one the most lethal malignancies women. serine protease dipeptidyl peptidase 4 (DPP4) plays key roles metabolism immunity, its expression been associated with either pro- or anti-tumour effects multiple tumour types. In this study, we provide first evidence that DPP4 enzyme activity are uncoupled under hypoxic conditions cells. Whilst identified strong up-regulation mRNA growth, specific secreted was...
Ovarian cancer remains the most lethal of gynecological malignancies, with 5-year survival below 50%. Currently there is no simple and effective pre-surgical diagnosis or triage for patients malignancy, particularly those early-stage low-volume tumors. Recently we discovered that CXCL10 can be processed to an inactive form in ovarian cancers its measurement has diagnostic significance. In this study evaluated addition a biomarker panel discrimination benign from malignant disease. Multiple...
Ovarian cancers (OCs) are the most lethal gynaecological malignancy, with high levels of relapse and acquired chemo-resistance. Whilst tumour⁻immune nexus controls both cancer progression regression, lack an appropriate system to accurately model tumour stage immune status has hampered validation clinically relevant immunotherapies therapeutic vaccines date. To address this need, we stably integrated near-infrared phytochrome iRFP720 at
Leader cells are a subset of cancer that coordinate the complex cell-cell and cell-matrix interactions required for ovarian migration, invasion, tumour deposition negatively associated with progression-free survival response to therapy. Emerging evidence suggests leader may be enriched in chemotherapy, underlying disease recurrence following treatment.CRISPR was used insert bicistronic T2A-GFP cassette under native KRT14 (leader cell) promoter. 2D 3D drug screens were completed presence...
Ovarian cancers metastasize by shedding into the peritoneal fluid and dispersing to distal sites within peritoneum. Monolayer cultures do not accurately model behaviors of cancer cells a nonadherent environment, as inherently aggregate multicellular structures which contribute metastatic process attaching invading lining form secondary tumors. To this important stage ovarian metastasis, aggregates, or spheroids, can be generated from established cell lines maintained under conditions. mimic...
Ovarian cancers metastasize by shedding into the peritoneal fluid and dispersing to distal sites within peritoneum. Monolayer cultures do not accurately model behaviors of cancer cells a nonadherent environment, as inherently aggregate multicellular structures which contribute metastatic process attaching invading lining form secondary tumors. To this important stage ovarian metastasis, aggregates, or spheroids, can be generated from established cell lines maintained under conditions. mimic...