A5008102725- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Neonatal Respiratory Health Research
- Malaria Research and Control
- Immune cells in cancer
- Cystic Fibrosis Research Advances
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Cerebrovascular and genetic disorders
- Vitamin D Research Studies
- SARS-CoV-2 and COVID-19 Research
- Cancer Immunotherapy and Biomarkers
- Tracheal and airway disorders
- Complement system in diseases
- Respiratory viral infections research
- Immune Response and Inflammation
- Acute Ischemic Stroke Management
- T-cell and B-cell Immunology
- Parathyroid Disorders and Treatments
- Immune responses and vaccinations
- Moyamoya disease diagnosis and treatment
- Metabolomics and Mass Spectrometry Studies
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
RMIT University
2018-2024
Royal Hospital for Children
2023
NHS Lothian
2021-2022
Monash University
2015-2020
University of Edinburgh
2013-2020
UK Biobank
2020
University of Oxford
2020
Queen Margaret University
2019
University of Sheffield
2010-2014
In UK Biobank (UKB), a large population-based prospective study, cases of many diseases are ascertained through linkage to routinely collected, coded national health datasets. We assessed the accuracy these for identifying incident strokes.In regional UKB subpopulation (n = 17,249), we identified all participants with ≥1 code signifying first stroke after recruitment (incident stroke-coded cases) in linked hospital admission, primary care, or death record data. Stroke physicians reviewed...
In-vitro studies suggest that vitamin D reduces inflammation by inhibiting nuclear factor kappa-B (NFκB) activity. Yet, no trials have examined the effects of supplementation on NFκB activity in-vivo in humans. We conducted a double-blind randomized trial (RCT) examining inflammatory markers and peripheral blood mononuclear cells (PBMCs). Sixty-five overweight/obese, D-deficient (25-hydroxyvitamin [25(OH)D] ≤ 50 nmol/L) adults were to single 100,000 IU bolus followed 4,000 daily...
Long PLUNC1 (LPLUNC1, C20orf114) is a member of family poorly described proteins (PLUNCS) expressed in the upper respiratory tract and oral cavity, which may function host defence. Although it one most highly genes airways has been identified sputum nasal secretions by proteomic studies, localisation LPLUNC1 protein not yet described. We developed affinity purified antibodies localised tissues human tract, oro- nasopharynx. have complemented these studies with analysis expression primary...
Immunity plays a key role in epithelial ovarian cancer (EOC) progression with well-documented correlation between patient survival and high intratumoral CD8+ to T regulatory cell (Treg) ratios. We previously identified dysregulated DPP4 activity EOCs as potentially immune-disruptive influence contributing reduction CXCR3-mediated T-cell infiltration solid tumours. therefore hypothesized that inhibition of by sitagliptin, an FDA-approved inhibitor, would improve function syngeneic ID8 mouse...
Insulin resistance (IR) and chronic low-grade inflammation are risk factors for diseases including type 2 diabetes (T2D) cardiovascular disease. This study aimed to investigate two dietary indices: Mediterranean Diet Score (MDS) Dietary Inflammatory Index (DII®), their associations with direct measures of glucose metabolism adiposity, biochemical lipids, cytokines adipokines in overweight/obese adults. cross-sectional included 65 participants (males = 63%; age 31.3 ± 8.5 years). intake via...
Despite being initially identified in mice, little is known about the sites of production members BPI fold (BPIF) containing (PLUNC) family putative innate defence proteins this species. These have largely been considered to be specificaly expressed respiratory tract, and we recently shown that they exhibit differential expression epithelium proximal airways. In study, used species-specific antibodies systematically localize two protein family; BPIFA1 (PLUNC/SPLUNC1) BPIFB1 (LPLUNC1) adult...
The development of practical and flexible vaccines to target liver stage malaria parasites would benefit from an ability induce high levels CD8 T cells minimal peptide epitopes. Herein we compare different adjuvant carrier systems in a murine model for induction interferon gamma (IFN-γ) producing the immuno-dominant epitope circumsporozoite protein (CSP) Plasmodium berghei, pb9 (SYIPSAEKI, referred as KI). Two pro-inflammatory adjuvants, Montanide Poly I:C, non-classical, non-inflammatory...
Gynecological cancers are a leading cause of mortality in women. CD8++ T cell immunity largely correlates with enhanced survival, whereas inflammation is associated poor prognosis. Previous studies have shown polystyrene nanoparticles (PSNPs) biocompatible, do not induce and when used as vaccine carriers for model peptides CD8+ responses. Herein we test the immunogenicity 24 different peptides, from three target proteins gynecological cancers: E7 protein human papilloma virus (HPV); Wilms...
Malaria remains a significant health problem in many tropical and sub-tropical regions. The development of vaccines against the clinically active blood-stage infection needs to consider variability polymorphism target antigens, an adjuvant system able induce broad spectrum immunity comprising both antibodies helper T cells. Moreover, recent studies have shown some conventional pro-inflammatory adjuvants can also promote expansion immunosuppressive regulatory cells (Treg) myeloid derived...
Although the biology PLUNC (recently renamed BPI fold, BPIF) family of secreted proteins is poorly understood, multiple array based studies have suggested that some are differentially expressed in lung diseases. We examined expression BPIFB1 (LPLUNC1), prototypic two-domain containing member, lungs from CF patients and mouse models disease. was localized samples along with BPIFA1, MUC5AC, CD68 NE directly compared to histologically normal tissues bacterial pneumonia. generated novel...
DNA vaccines offer cost, flexibility, and stability advantages, but administered alone have limited immunogenicity. Previously, we identified optimal configurations of magnetic vectors comprising superparamagnetic iron oxide nanoparticles (SPIONs), polyethylenimine (PEI), hyaluronic acid (HA) to deliver malaria encoding Plasmodium yoelii (Py) merozoite surface protein MSP119 (SPIONs/PEI/DNA + HA gene complex) dendritic cells transfect them with high efficiency in vitro. Herein, evaluate...
Adipokines play an important role in the regulation of glucose metabolism. We have previously shown that carnosine supplementation overweight or obese non-diabetic individuals improves metabolism but does not change adiponectin concentrations. However, its effect on other adipokines has been investigated. Herein we further determined serum adipsin, resistin and leptin. Twenty-two otherwise healthy adults were randomly assigned to receive either 2 g (n = 13) identically looking placebo 9) for...
Ovarian cancers (OCs) are the most lethal gynaecological malignancy, with high levels of relapse and acquired chemo-resistance. Whilst tumour⁻immune nexus controls both cancer progression regression, lack an appropriate system to accurately model tumour stage immune status has hampered validation clinically relevant immunotherapies therapeutic vaccines date. To address this need, we stably integrated near-infrared phytochrome iRFP720 at