A5067444550- DNA Repair Mechanisms
- RNA Research and Splicing
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Sperm and Testicular Function
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Nuclear Structure and Function
- RNA and protein synthesis mechanisms
- Influenza Virus Research Studies
- Prenatal Screening and Diagnostics
- interferon and immune responses
- Viral Infections and Immunology Research
- Click Chemistry and Applications
- Genetics and Neurodevelopmental Disorders
- Immune Response and Inflammation
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Biotin and Related Studies
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Gut microbiota and health
- Sexual Differentiation and Disorders
Wuhan University
2008-2024
Hubei Provincial Center for Disease Control and Prevention
2018-2021
University of Pennsylvania
2013-2020
YTHDF1 is known as a m6 A reader protein, and many researches of focused on the regulation mRNA translation efficiency. However, also related to RNA degradation, but how regulates degradation indefinite. Liquid-liquid phase separation (LLPS) underlies formation membraneless compartments in mammal cells, there are few reports correlation with LLPS. In this research, we mechanism degraded through LLPS.The CRISPR/Cas9 knock out system was used establish (YTHDF1-KO) cell lines (HEK293 HeLa)...
Meiotic recombination permits exchange of genetic material between homologous chromosomes. The replication protein A (RPA) complex, the predominant ssDNA-binding is required for nearly all aspects DNA metabolism, but its role in mammalian meiotic remains unknown due to embryonic lethality RPA mutant mice. a heterotrimer RPA1, RPA2, and RPA3. We find that loss largest subunit, leads disappearance RPA2 RPA3, resulting absence complex. Using an inducible germline-specific inactivation strategy,...
Abstract DNA methylation, repressive histone marks, and PIWI-interacting RNA (piRNA) are essential for the control of retrotransposon silencing in mammalian germline. However, it remains unknown how these epigenetic pathways crosstalk to ensure male Here, we show that UHRF1 is responsible cooperates with germ cells. Conditional loss postnatal cells causes hypomethylation, upregulation retrotransposons, activation a damage response, switches global chromatin status, leading complete...
Polycomb group proteins mediate transcriptional silencing in diverse developmental processes. Sex chromosomes undergo chromosome-wide transcription during male meiosis. Here we report that mouse SCML2 (Sex comb on midleg-like 2), an X chromosome-encoded polycomb protein, is specifically expressed germ cells, including spermatogonia, spermatocytes, and round spermatids. associates with phosphorylated H2AX localizes to the XY body spermatocytes. Loss of mice causes defective spermatogenesis,...
SKP1 coordinates chromosome synapsis and meiotic recombination with progression through the first cell division in mouse.
The chromatin state, which undergoes global changes during spermatogenesis, is critical to meiotic initiation and progression. However, the key regulators involved underlying molecular mechanisms remain be uncovered. Here, we report that mouse BEND2 specifically expressed in spermatogenic cells around it plays an essential role
Primary ovarian insufficiency (POI), also known as premature failure, is an defect in humans characterized by the depletion of follicles before age 40. However, mechanisms underlying POI remain largely unknown. Here, we show that knockout Epg5 (ectopic P-granules autophagy protein 5 homolog (C. elegans)) results subfertility female mice, which exhibit a POI-like phenotype. Single-cell RNA sequencing analysis revealed affected differentiation granulosa cells (GCs). Further investigation...
Self-renewal of spermatogonial stem cells is vital to lifelong production male gametes and thus fertility. However, the underlying mechanisms remain enigmatic. Here, we show that DOT1L, sole H3K79 methyltransferase, required for cell self-renewal. Mice lacking DOT1L fail maintain cells, characterized by a sequential loss germ from spermatogonia spermatids ultimately Sertoli only syndrome. Inhibition reduces activity after transplantation. promotes expression fate-determining HoxC...
Abstract Prenatal dexamethasone exposure (PDE) induces multiorgan developmental toxicities in offspring. Here we verified the transgenerational inheritance effect of ovarian toxicity by PDE and explored its intrauterine programming mechanism. Pregnant rats subcutaneously received 0.2 mg/kg/d from gestational day (GD) 9 to GD20. A subgroup was euthanized for fetuses on GD20, other group went spontaneous labor produce F1 The adult females were mated with normal males F2 F3 generations. fetal...
Mechanisms driving the prolonged meiotic prophase I in mammals are poorly understood. RNA helicase YTHDC2 is critical for mitosis to meiosis transition. However, highly expressed pachytene cells. Here we identify an essential role progression. Specifically, deficiency causes microtubule-dependent telomere clustering and apoptosis at stage of I. Depletion results a massively dysregulated transcriptome cells, with tendency toward upregulation genes normally mitotic germ cells downregulation...
Abstract Homeostasis of meiotic DNA double strand breaks (DSB) is critical for germline genome integrity and homologous recombination. Here we demonstrate an essential role SKP1, a constitutive subunit the SCF (SKP1-Cullin-F-box) ubiquitin E3 ligase, in early processes. SKP1 restrains accumulation HORMAD1 pre-DSB complex (IHO1-REC114-MEI4) on chromosome axis germ cells. Loss prior to meiosis leads aberrant localization DSB repair proteins failure synapsis initiation both males females....
Highly pathogenic avian influenza H5N1 virus represents a growing threat for an pandemic. Development of effective vaccines is priority pandemic preparedness. Focusing on virus-like particles (VLPs) has been suggested as promising vaccine approach. Recent VLP vaccination efforts have concentrated the strains isolated from humans. Because all confirmed cases human infection were directly transmitted infected poultry, it interest to develop comprised antigenic proteins in order compare their...
The increasing emergence of Cas9 variants has attracted broad interest, as these were designed to expand CRISPR applications. New typically feature higher editing efficiency, improved specificity, or alternative PAM sequences. To select and gRNAs for high-fidelity efficient genome editing, it is crucial systematically quantify the performances develop prediction models based on high-quality datasets. Using synthetic gRNA-target paired libraries next-generation sequencing, we compared...
RNA regulation by RNA-binding proteins (RBPs) involve extremely complicated mechanisms. MOV10 and MOV10L1 are two homologous helicases implicated in distinct intracellular pathways. participates specifically Piwi-interacting (piRNA) biogenesis protects mouse male fertility. In contrast, the functional complexity of remains incompletely understood, its role mammalian germline is unknown. Here, we report a study biological molecular functions helicase germ cells. nucleocytoplasmic protein...
Histone modifications regulate chromatin remodeling and gene expression in development diseases. DOT1L, the sole histone H3K79 methyltransferase, is essential for embryonic development. Here, we report that DOT1L regulates male fertility mouse. associates with MLLT10 testis. localize to sex meiotic post-meiotic germ cells an inter-dependent manner. Loss of either or leads reduced testis weight, decreased sperm count subfertility. H3K79me2 abundant elongating spermatids, which undergo...
Abstract Proper nutrition is essential for sustaining optimal reproductive function in both humans and ruminants. Studies have shown that undernutrition overnutrition can negatively impact human processes. However, the Mediterranean diet, with its balanced nutrient‐rich composition, recognised positive effects on reproduction. In context of ruminants, a well‐balanced diet equally crucial achieving peak performance. Grazing, which allows ruminants to feed natural pasture open environments,...
Meiosis is a specialized cell division for producing haploid gametes from diploid germ cells. During meiosis, synaptonemal complex (SC) mediates the alignment of homologs and plays essential roles in homologous recombination therefore promoting accurate chromosome segregation. In this study, we have identified novel protein SCRE (synaptonemal reinforcing element) as key molecule maintaining integrity SC during meiosis prophase I mice. Deletion Scre caused death both male female mice,...
Continual spermatogenesis relies on the actions of an undifferentiated spermatogonial population that is composed stem cells and progenitors. Here, using mouse models, we explored role RNA-binding proteins (RBPs) in regulation biological activities this population. Proteins bound to polyadenylated RNAs primary cultures spermatogonia were captured with oligo (dT)-conjugated beads after UV-crosslinking profiled by proteomics (termed mRBPome capture), yielding a putative repertoire 473 RBPs....