A5086943203- Acute Myeloid Leukemia Research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Acute Lymphoblastic Leukemia research
- Cancer-related gene regulation
- Hematopoietic Stem Cell Transplantation
- Hematological disorders and diagnostics
- Birth, Development, and Health
- Chronic Lymphocytic Leukemia Research
- Childhood Cancer Survivors' Quality of Life
- Prenatal Screening and Diagnostics
- DNA Repair Mechanisms
- Blood disorders and treatments
- Cancer-related molecular mechanisms research
- Reproductive Biology and Fertility
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Hemoglobinopathies and Related Disorders
- Chronic Myeloid Leukemia Treatments
- Porphyrin Metabolism and Disorders
- Folate and B Vitamins Research
- RNA Research and Splicing
- Erythrocyte Function and Pathophysiology
- Osteoarthritis Treatment and Mechanisms
- Transplantation: Methods and Outcomes
Institute of Hematology & Blood Diseases Hospital
2016-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2024
Wuhan University
2017-2024
Academy of Medical Sciences
2024
Peking Union Medical College Hospital
2023
Jiangxi University of Traditional Chinese Medicine
2021
Nanjing University
2020
Nanjing General Hospital of Nanjing Military Command
2020
Fuyang City People's Hospital
2020
The First Affiliated Hospital, Sun Yat-sen University
2019-2020
Mouse embryonic fibroblasts (MEFs) can be differentiated into fully functional chondrocytes in response to bone morphogenetic protein-2 (BMP-2). The expression of Sox9, a critical transcription factor for the multiple steps chondrogenesis, has been reported upregulated during this process. But molecular mechanisms by which BMP-2 promotes chondrogenesis still remain largely unknown. aim present study was therefore investigate underlying mechanism. In MEFs, efficiently induced Sox9 along with...
Abstract Prenatal dexamethasone exposure (PDE) induces multiorgan developmental toxicities in offspring. Here we verified the transgenerational inheritance effect of ovarian toxicity by PDE and explored its intrauterine programming mechanism. Pregnant rats subcutaneously received 0.2 mg/kg/d from gestational day (GD) 9 to GD20. A subgroup was euthanized for fetuses on GD20, other group went spontaneous labor produce F1 The adult females were mated with normal males F2 F3 generations. fetal...
Ribosomal protein dysfunction causes diverse human diseases, including Diamond-Blackfan anemia (DBA). Despite the universal need for ribosomes in all cell types, mechanisms underlying ribosomopathies, which are characterized by tissue-specific defects, still poorly understood. In present study, we analyzed transcriptomes of single purified erythroid progenitors isolated from bone marrow DBA patients. These patients were categorized into untreated, glucocorticoid (GC)-responsive and...
Background Cytomegalovirus (CMV) infection is a common and life-threatening complication following allogeneic haematopoietic stem cell transplantation (allo-HSCT). Letermovir (LET) has been the standard prophylaxis for adult recipients, but studies in children remain limited. Methods We retrospectively analyzed with or without LET after haploidentical donor (HID) Beijing protocol unrelated cord blood (UCB) transplantation. Results Of 151 patients, 67 received LET, including 35 HID recipients...
Diamond–Blackfan anemia (DBA) was the first ribosomopathy associated with mutations in ribosome protein (RP) genes. The clinical phenotypes of DBA include failure erythropoiesis, congenital anomalies and cancer predisposition. Mutations RPL5 are reported approximately 9 ~ 21 % patients, which represents most common pathological condition related to a large-subunit ribosomal protein. However, it remains unclear how downregulation results severe this disease. In study, we generated zebrafish...
The first intronic mutations in the intron 1 GATA site (int-1-GATA) of 5-aminolevulinate synthase 2 (ALAS2) have been identified X-linked sideroblastic anemia (XLSA) pedigrees, strongly suggesting it could be causal XLSA. However, function this int-1-GATA during vivo development remains largely unknown. Here, we generated mice lacking a 13 bp fragment, including (T AGATAA: AGCCCC) and found that hemizygous deletion led to an embryonic lethal phenotype due severe resulting from lack ALAS2...
Abstract Inherited non-hemolytic anemia is a group of rare bone marrow disorders characterized by erythroid defects. Although concerted efforts have been made to explore the underlying pathogenetic mechanisms these diseases, understanding causative mutations are still incomplete. Here we identify in diseased pedigree that gain-of-function mutation toll-like receptor 8 ( TLR8 ) implicated inherited anemia. expressed lineage and erythropoiesis impaired activation whereas enhanced inhibition...
A previous study has shown that UV activates the PI3K/AKT cell survival pathway while inducing death in human skin vivo and cultured keratinocytes vitro, yet upstream leading to activation of AKT not been thoroughly investigated. In this we found UV-induced phosphorylation p38 a time-dependent manner. The started at 5 min post irradiation, peaked about 30 min, remained elevated up 2 h. 15 treatment, 1 h, We also H2O2 induced time- dependent Pretreatment with NAC abolished phosphorylation,...
MSCs-based therapy for cancer is a relatively new but rapidly growing area of research.Human term placenta, an attractive source MSCs (PMSCs), appears to have great advantage due its easy access without invasive procedures, lack ethical issues and high-throughput young age.In the present study, we isolated from placenta characterized their morphology differentiation capacities.We next investigated underlying antitumor effects potential mechanism PMSCs express endostatin using adenoviral...
Abstract While Polycomb group protein Bmi1 is important for stem cell maintenance, its role in lineage commitment largely unknown. We have identified as a novel regulator of erythroid development. highly expressed mouse progenitor cells and deficiency impairs differentiation. BMI1 also human Furthermore, we discovered that loss results decreased transcription multiple ribosomal genes impaired ribosome biogenesis. stabilizes p53 protein, leading to upregulation p21 expression subsequent G0/G1...
Background Primary myelofibrosis (PMF) is quite rare in children. Mutations of JAK2 V617F or MPL W515K/L were absent pediatric patients with PMF according to previous studies. Recently, mutations calreticulin ( CALR ) described adult / ‐unmutated PMF. Our study aimed analyze the clinical and genetic features Chinese Procedures We retrospectively investigated 14 diagnosed as WHO 2008 criteria. Direct sequencing was performed for existence alterations JAK2, MPL, TET2, CBL, ASXL1, IDH1, IDH2,...
Abstract Objective To evaluate the clinical value of positive copy number variations (CNVs) results by non‐invasive prenatal testing (NIPT) without fetal ultrasonography‐identified structural anomalies, especially with several known CNVs results. Methods A total 135,981 NIPT performed between April 1, 2017, and March 31, 2020, enrolled in free service program implemented local government were retrospectively analyzed. Of these, 87 cases screens for no anomalies recalled provided genetic...
Abstract Background Diamond–Blackfan anemia is a rare congenital red blood cell dysplasia that develops soon after birth. RPL11 mutations account for approximately 4.8% of human DBA cases with defective hematopoietic phenotypes. However, the mechanisms by which regulates hematopoiesis in remain elusive. In this study, we analyzed transcriptome using deep sequencing data from an Rpl11-deficient zebrafish model to identify Rpl11-mediated failure and investigate underlying mechanisms. Results...
Diamond-Blackfan anemia (DBA) is a rare inherited bone marrow failure syndrome that characterized by pure red-cell aplasia and associated physical deformities. It has been proven defects of ribosomal proteins can lead to this disease RPS19 the most frequently mutated gene in DBA patients. Previous studies suggest p53-dependent genes pathways play important roles RPS19-deficient embryos. However, whether there are other vital factors linked not fully clarified. In study, we compared whole...
Diamond–Blackfan anemia (DBA) is a class of human diseases linked to defective ribosome biogenesis that results in clinical phenotypes. Genetic mutations protein (RP) genes lead DBA phenotypes, including hematopoietic defects and physical deformities. However, little known about the global regulatory network as well key miRNAs gene pathways zebrafish model DBA. In this study, we establish using an RPS24 morpholino found required for both primitive hematopoiesis definitive processes are...
Objective This study aimed to compare the health economic value of a non-invasive prenatal testing (NIPT) strategy against second-trimester triple screening (STS) for detection Down syndrome based on real-world data from China. Design A decision-analytical model was developed cost-effectiveness five strategies societal perspective. Cost and probability input were obtained surveys published sources. Setting Participants Women with singleton pregnancy. Interventions The were: (A) maternal age...
Early treatment responses are important prognostic factors in childhood T-cell acute lymphoblastic leukemia (T-ALL) patients. The predictive values of early Chinese T-ALL patients were still unknown.From January 2003 to December 2012, 74 consecutive aged ≤ 15 years with newly diagnosed treated BCH-2003 protocol or CCLG-2008 the Department Pediatric, Institute Hematology and Blood Diseases Hospital China. Predictive responses, including prednisone response, bone marrow morphology at day 33...
Abstract Background Contemporary risk‐directed treatment has improved the outcome of patients with acute lymphoblastic leukemia (ALL) and TCF3::PBX1 fusion. In this study, authors seek to identify prognostic factors that can be used further improve outcome. Methods The studied 384 genotype treated on Chinese Children's Cancer Group ALL‐2015 protocol between January 1, 2015 December 31, 2019. All provisionally received intensified chemotherapy in intermediate‐risk arm without prophylactic...
Congenital sideroblastic anemias (CSAs) comprise a group of heterogenous genetic diseases that are caused by the mutation various genes involved in heme biosynthesis, iron-sulfur cluster biogenesis, or mitochondrial solute transport metabolism. However, approximately 40 % patients with CSA have not been found to pathogenic gene mutations. In this study, we systematically analyzed profile 10 Chinese sporadic CSA. We performed targeted deep sequencing analysis ten using panel 417 included...