A5113103263- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Chronic Kidney Disease and Diabetes
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Kruppel-like factors research
- Molecular Biology Techniques and Applications
- Systemic Lupus Erythematosus Research
- Renal Diseases and Glomerulopathies
- Renal and related cancers
- Ferroptosis and cancer prognosis
- Bioinformatics and Genomic Networks
- Chronic Myeloid Leukemia Treatments
- Forensic and Genetic Research
- Lung Cancer Research Studies
- Prenatal Screening and Diagnostics
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
Nanchang University
2014-2025
Beijing Institute of Genomics
2015-2024
Chinese Academy of Sciences
2015-2024
University of Chinese Academy of Sciences
2010-2024
Second Affiliated Hospital of Nanchang University
2011-2024
Shanghai Center For Bioinformation Technology
2023-2024
Peking University
2023-2024
Peking University People's Hospital
2024
Sino-Danish Centre for Education and Research
2020-2023
Dazhou Central Hospital
2018-2022
Abstract With the rapid development of sequencing technologies towards higher throughput and lower cost, sequence data are generated at an unprecedentedly explosive rate. To provide efficient easy-to-use platform for managing huge data, here we present Genome Sequence Archive (GSA; http://bigd.big.ac.cn/gsa or http://gsa.big.ac.cn), a repository archiving raw data. In compliance with standards structures International Nucleotide Database Collaboration (INSDC), GSA adopts four objects...
Malaria erythrocyte binding proteins use the Duffy blood group antigen (Plasmodium vivax and Plasmodium knowlesi) sialic acid falciparum) on surface as receptors. We had previously cloned one P. gene, falciparum part of three knowlesi genes encoding these described homology between genes. have completed cloning sequencing identified introns in that correct published deduced amino sequences. All similar structures, with or two exons signal sequence domain, an exon transmembrane cytoplasmic...
Abstract The National Genomics Data Center (NGDC), which is a part of the China for Bioinformation (CNCB), provides family database resources to support global academic and industrial communities. With rapid accumulation multi-omics data at an unprecedented pace, CNCB-NGDC continuously expands updates core through big archiving, integrative analysis value-added curation. Importantly, NGDC collaborates closely with major international databases initiatives ensure seamless exchange...
Minimal change disease (MCD) is a podocytopathy more commonly seen in children, but it also accounts for 10%-25% of adult nephrotic syndrome. High-dose oral glucocorticoids were recommended initial treatment MCD. However, long-term use systemic corticosteroids associated with significant adverse events, such as steroid-induced diabetes and infections. The aim this study was to investigate the clinical efficacy safety half-dose combined rituximab (RTX) We recruited 74 patients MCD confirmed...
Cultured human umbilical cord mesenchymal stem cells (hUC-MSCs) are being tested in several clinical trials and encouraging outcomes have been observed. To determine whether vitro expansion influences the genomic stability of hUC-MSCs, we maintained nine hUC-MSC clones long-term culture comparatively analyzed them at early late passages. All senesced culture, exhibiting decreased telomerase activity shortened telomeres. Two showed no DNA copy number variations (CNVs) passage 30 (P30). Seven...
Abstract High deviations resulting from prediction model, gender and population difference have limited age estimation application of DNA methylation markers. Here we identified 2,957 novel age-associated sites ( P < 0.01 R 2 > 0.5) in blood eight pairs Chinese Han female monozygotic twins. Among them, nine (false discovery rate 0.01), along with three other reported sites, were further validated 49 unrelated volunteers ages 20–80 years by Sequenom Massarray. A total 95 CpGs covered...
P-glycoprotein (P-gp) can actively pump paclitaxel (PTX) out of cells and induces drug resistance. Abraxane, a nanoparticle (NP) formulation PTX, has multiple clinical advantages over the single molecule form. However, it is still unclear whether Abraxane overcomes common small resistance problem mediated by P-gp. Here we were able to establish an Abraxane-resistant cell line from lung adenocarcinoma A549. We compared transcriptome A549/Abr resistant that its parental using RNA-Seq...
To evaluate the effect and safety of acupuncture for treatment irritable bowel syndrome (IBS) through comparisons with those polyethylene glycol (PEG) 4000 pinaverium bromide.This multicenter randomized controlled trial was conducted at 7 hospitals in China enrolled participants who met Rome III diagnostic criteria IBS between May 3, 2015, June 29, 2018. Participants were first stratified into constipation-predominant or diarrhea-predominant group. each group randomly assigned a 2:1 ratio to...
// Junyun Wang 1,2,* , Nan Ding Yongjun Li 1,* Hua Cheng 3,* Dong 4 Qiong Yang 1,2 Youhui Deng Yaran 1 Yanming Xiuyan Ruan Fang Xie Zhao and Xiangdong CAS Key Laboratory of Genome Sciences Information, Beijing Institute Genomics, Chinese Academy Sciences, Beijing, China 2 University 3 Biology, Hebei Shijiazhuang, Department Dermatology, General Hospital People's Liberation Army, * These authors have contributed equally to this work Correspondence to: Fang, email: Zhao, Keywords : IGFBP5;...
The emergence and rapid spreading of multidrug-resistant Acinetobacter baumannii strains has become a major health threat worldwide. To better understand the genetic recombination related with acquisition drug-resistant elements during bacterial infection, we performed complete genome analysis on three newly isolated A. from Beijing using next-generation sequencing technology.Whole comparison revealed that all 3 share some common drug resistant including carbapenem-resistant bla OXA-23...
MicroRNA-34a (miR-34a) is frequently downregulated in pancreatic ductal adenocarcinoma (PDAC) cells, however, the silencing mechanism remains unclear. Enhancer of zeste homolog 2 (EZH2) overexpressed PDAC, and our previous miRNA profiling showed that inhibition EZH2 PDAC cells led to re-expression a group tumor suppressor miRNAs including miR-34a. Here, we studied effect ectopic expression miR-34a, identified HOTAIR as an interacting partner induce heterochromatin formation during miR-34a...
Background: The variations and their correlation of inflammation oxidative stress in chronic kidney disease (CKD) have not been thoroughly understood. Materials methods: Biomarkers were measured a cohort 176 patients with CKD ranging from stage 1 to 5 67 healthy controls. Correlation analysis levels between was also performed estimated glomerular filtration rate (eGFR) using the Modification Diet Renal Disease (MDRD) formula. Concentrations serum creatinine (Scr), hs-CRP (hypersensitive C...
Abstract To unravel the genetic mechanisms of disease and physiological traits, it requires comprehensive sequencing analysis large sample size in Chinese populations. Here, we report primary results Academy Sciences Precision Medicine Initiative (CASPMI) project launched by Sciences, including de novo assembly a northern Han reference genome (NH1.0) whole analyses 597 healthy people coming from most areas China. Given two existing genomes for (YH HX1) were both south, constructed NH1.0, new...
Spin-selective absorption is broadly applicable to numerous photonic devices. Here, based on a stereoscopic full metallic resonator array, terahertz chiral metasurface with single-layer structure proposed and numerically demonstrated. By employing the coupled-mode theory, we demonstrate that can near-perfectly absorb one circularly polarized wave in quasi-bound states continuum-induced critical coupling region but non-resonantly reflect its counterparts. Interestingly, linewidths handedness...
Hyperglycemia accelerates the development of diabetic nephropathy (DN) by inducing renal tubular injury. Nevertheless, mechanism has not been elaborated fully. Here, pathogenesis DN was investigated to seek novel treatment strategies.A model established in vivo, levels blood glucose, urine albumin creatinine ratio (ACR), creatinine, urea nitrogen (BUN), malondialdehyde (MDA), glutathione (GSH), and iron were measured. The expression detected qRT-PCR Western blotting. H&E, Masson, PAS...
Abstract Single-cell RNA sequencing methods focusing on the 5′-end of transcripts can reveal promoter and enhancer activity efficiently profile immune receptor repertoire. However, ultra-high-throughput single-cell have not been described. We introduce FIPRESCI, combinatorial indexing RNA-Seq, enabling massive sample multiplexing increasing throughput droplet microfluidics system by over tenfold. demonstrate FIPRESCI enables generation approximately 100,000 transcriptomes from E10.5 whole...
Purpose The controversial nature of the association between α-Klotho and mortality risk in general population warrants further investigation. This study aims to examine correlation circulating levels all-cause Methods A sample size 13,748 individuals from NAHNES 2005-2016 cycles was included this study. effect different (divided into quartiles) on survival assessed using Kaplan-Meier (KM) curves. Cox proportional hazards models were used analyze linear relationship log mortality. Restricted...