A5064607503- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- Environmental DNA in Biodiversity Studies
- Marine and coastal ecosystems
- Cellular Mechanics and Interactions
- Network Security and Intrusion Detection
- Epigenetics and DNA Methylation
- Explainable Artificial Intelligence (XAI)
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Genetic and Kidney Cyst Diseases
- Software-Defined Networks and 5G
- Phytoplasmas and Hemiptera pathogens
- Autophagy in Disease and Therapy
- Microtubule and mitosis dynamics
- Diatoms and Algae Research
- Advanced Malware Detection Techniques
- Neural Networks and Applications
- Global Energy and Sustainability Research
- Cellular transport and secretion
- Parasitic Infections and Diagnostics
- Algal biology and biofuel production
- Big Data and Business Intelligence
- Genetic Mapping and Diversity in Plants and Animals
Institute of Hydrobiology
2016-2025
Chinese Academy of Sciences
2016-2025
Gulf University
2024
Sinotech Genomics (China)
2024
Beibu Gulf University
2024
Dalian Ocean University
2019-2021
Shenzhen Stock Exchange
2021
Guangdong Ocean University
2019-2021
University of Chinese Academy of Sciences
2018-2019
Shandong University of Science and Technology
2019
Abstract The National Genomics Data Center (NGDC), which is a part of the China for Bioinformation (CNCB), provides family database resources to support global academic and industrial communities. With rapid accumulation multi-omics data at an unprecedented pace, CNCB-NGDC continuously expands updates core through big archiving, integrative analysis value-added curation. Importantly, NGDC collaborates closely with major international databases initiatives ensure seamless exchange...
The Tibetan Plateau supplies water to nearly 2 billion people in Asia, but climate change poses threats its aquatic microbial resources. Here, we construct the Microbial Catalog by sequencing 498 metagenomes from six ecosystems (saline lakes, freshwater rivers, hot springs, wetlands and glaciers). Our catalog expands knowledge of regional genomic diversity presenting 32,355 metagenome-assembled genomes that de-replicated into 10,723 representative genome-based species, which 88% were...
Abstract Certain ciliates of the subclass Scuticociliatia (scuticociliates) are facultative parasites fishes in which they cause a suite diseases collectively termed scuticociliatosis. Hitherto, comparatively little was known about genetics and genomics scuticociliates or mechanism In this study, laboratory culture facultatively pathogenic scuticociliate Pseudocohnilembus persalinus established its genome sequenced, giving first marine ciliate. Genome-wide horizontal gene transfer (HGT)...
Deep learning is a subset of machine that models data using artificial neural networks with multiple layers. Each layer in the network processes input data, extracts relevant features, and passes it to next layer. techniques have led significant advancements areas such as image recognition, natural language processing, speech recognition.
Geothermal spring ecosystems, as extreme habitats, exert significant environmental pressure on their microeukaryotic communities. However, existing studies the stability of communities in geothermal ecosystems across different habitats and temperature gradients are still limited. In this study, we used high-throughput 18S rDNA sequencing combination with factor analysis to investigate co-occurrence patterns, assembly mechanisms, responses changes sediment water samples from 36 springs...
ABSTRACT Ciliates represent a diverse assemblage of ancient single‐celled eukaryotes characterized by morphological features. Among certain sessilid peritrich ciliates, an exceptional structure known as the stalk has been documented since pioneering work Antonie van Leeuwenhoek in 17th century. This study conducts comparative genomic analysis three sessile species— Epistylis sp., Vorticella campanula , and Zoothamnium arbuscula —and two free‐swimming species, Tetrahymena thermophila...
A morphospecies is defined as a taxonomic species based wholly on morphology, but often consist of clusters cryptic that can be identified genetically or molecularly. The nature the evolutionary novelty accompanies speciation in an intriguing question. Morphospecies are particularly common among ciliates, group unicellular eukaryotes separates 2 kinds nuclei—the silenced germline nucleus (micronucleus [MIC]) and actively expressed somatic (macronucleus [MAC])—within cytoplasm. Because their...
The giant single-celled eukaryote, Spirostomum, exhibits one of the fastest movements in biological world. This ultrafast contraction is dependent on Ca2+ rather than ATP and therefore differs to actin-myosin system muscle. We obtained high-quality genome Spirostomum minus from which we identified key molecular components its contractile apparatus, including two major binding proteins (Spasmin 1 2) (GSBP1 GSBP2), act as backbone allow for hundreds spasmins. evidence suggests that...
Abstract Protists, a highly diverse group of microscopic eukaryotic organisms distinct from fungi, animals and plants, exert crucial roles within the earth's biosphere. However, genomes only small fraction known protist species have been published made publicly accessible. To address this constraint, Protist 10 000 Genomes Project (P10K) was initiated, implementing specialized pipeline for single-cell genome/transcriptome assembly, decontamination annotation protists. The resultant P10K...
Ciliates are a large group of ubiquitous and highly diverse single-celled eukaryotes that play an essential role in the functioning microbial food webs. However, their genomic diversity is far from clear due to need develop cultivation methods for most species, so research based on wild organisms almost invariably contain contaminants. Here we establish integrated Genome Decontamination Pipeline (iGDP) combines homology search, telomere reads-assisted clustering approaches filter...
Ciliates contain two kinds of nuclei: the germline micronucleus (MIC) and somatic macronucleus (MAC) in a single cell. The MAC usually have fragmented chromosomes. These chromosomes, capped with telomeres at both ends, could be gene size to several megabases length among different ciliate species. So far, no telomere-to-telomere assembly entire genome species has been finished. Development third generation sequencing technologies allows generate reads up that possibly span an chromosome....
Abstract Two populations of Epistylis chlorelligerum Shen 1980, a colonial limnetic peritrich ciliate, were collected from different locations in China: E. 1 West Lake, Hangzhou; 2 East Wuhan. The morphology, infraciliature and SSU rRNA gene sequence the two investigated based on living protargol‐stained specimens. Although both are consistent with previous descriptions specimens this species, some differences morphology vivo observed. had identical sequences. A second chrysemydis Bishop...
Abstract Resting cyst formation is a remarkable survival strategy used by ciliates in response to the adverse environmental conditions. However, mechanisms underlying encystment are poorly understood. Here, genetic basis of Colpoda aspera was examined through RNA sequencing identify transcriptome‐wide changes gene expression between vegetative and stages. After de novo assembly, 49,543 transcripts were identified. Gene annotation pathway mapping analysis revealed marked biosynthesis, energy...
Backup datacenters provide massive data storage and access services, their failure may result in huge economic losses. So location selection requires low damage risk high evacuation capability simultaneously. But previous works on backup datacenter placement have not jointly considered these two factors from the viewpoint of traffic engineering might unnecessary loss case disaster. In this paper, with global view network resources software defined scenarios, we propose a new...
Ciliates are fundamental components of microzooplankton, with important ecological roles. However, ciliate communities particularly difficult to monitor using conventional morphological approaches. New molecular tools, such as DNA metabarcoding, can facilitate the study these communities. This used high-throughput sequencing examine diversity and seasonal dynamics in four estuarine ecosystems South China Sea from June 2019 March 2020. The amplification V4 region 18S rDNA ciliate-specific...
Ciliated microeukaryotes (ciliates) are distinguished by their cilia, which rich in tubulin. We developed a method for tubulin staining ciliate cells that involves using live-cell tubulin-staining dyes instead of antibodies thereby streamlining the process, is effective across diverse lineages. Moreover, our allows integration with immunofluorescence when needed. The potential applications this technique extend to cell biology and morphological ecological studies.
Artificial Intelligence (AI) has permeated numerous aspects of our daily lives, from predictive text on smartphones to complex decision-making systems in healthcare and finance. While AI shown remarkable accuracy efficiency, it is often criticized for being a 'black box,' particularly when comes models like deep learning large language (LLMs). This where Explainable (XAI) into play.Explainable aims make decisions transparent, understandable, interpretable. The lack interpretability raised...
Hot springs are some of the most special environments on Earth. Many prokaryotic and eukaryotic microbes have been found to live in this environment. The Himalayan geothermal belt (HGB) has numerous hot spread across area. Comprehensive research using molecular techniques investigate microorganisms is still lacking; investigating composition diversity such as protists spring ecosystems will not only provide critical information adaptations extreme conditions, but could also give valuable...
In this paper, we present transcriptome data for Balantidium ctenopharyngodoni Chen, 1955 collected from the hindgut of grass carp (Ctenopharyngodon idella). We evaluated sequence quality and de novo assembled a preliminary transcriptome, including 43.3 megabits 119,141 transcripts. Then obtained final 17.7 35,560 transcripts, by removing contaminative redundant sequences. Phylogenomic analysis based on supermatrix with 132 genes comprising 53,873 amino acid residues phylogenetic SSU rDNA 27...
A limnetic peritrichous ciliate, Epistylis plicatilis Ehrenberg, 1831, was collected from a freshwater ditch beside Moshan Hill, Wuhan, China. Its morphology, infraciliature, and morphogenesis were investigated based on specimens examined in vivo, following staining with protargol by scanning electron microscopy. The characteristics of the Wuhan population E. are as follows: 1) colonial, each colony typically comprising 30–50 individuals, dichotomously branched, noncontractile stalk; 2)...