A5101781279- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related gene regulation
- Signaling Pathways in Disease
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Circular RNAs in diseases
- Prostate Cancer Diagnosis and Treatment
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
- Renal cell carcinoma treatment
- Cancer, Hypoxia, and Metabolism
- S100 Proteins and Annexins
- Bladder and Urothelial Cancer Treatments
- Cancer Genomics and Diagnostics
- Barrier Structure and Function Studies
- Mitochondrial Function and Pathology
- Cancer Immunotherapy and Biomarkers
- Inflammasome and immune disorders
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Chromosomal and Genetic Variations
Southern Medical University
2024
Nanfang Hospital
2024
Wuhan University
2002-2024
University of Electronic Science and Technology of China
2022-2024
People's Liberation Army 401 Hospital
2024
Yangtze University
2024
Tianjin Chest Hospital
2018-2024
Tianjin University
2024
Ruijin Hospital
2024
Shanghai Jiao Tong University
2024
Abstract Esophageal cancer is the sixth leading cause of death from and one least studied cancers worldwide. The global microRNA expression profile esophageal has not been reported previously. Here, for first time, we have investigated expressed microRNAs in cryopreserved tissues using advanced microarray techniques. Our analyses identified seven that could distinguish malignant lesions adjacent normal tissues. Some be correlated with different clinicopathologic classifications. High...
Recent studies have suggested that microRNA biomarkers could be useful for stratifying lung cancer subtypes, but signatures varied between different populations. Squamous cell carcinoma (SCC) is one major subtype of urgently needs to aid patient management. Here, we undertook the first comprehensive investigation on in Chinese SCC patients.MicroRNA expression was measured cancerous and noncancerous tissue pairs strictly collected from patients (stages I-III), who had not been treated with...
microRNAs (miRNAs) regulate gene expression at the post-transcriptional level and play important roles in tumor initiation progression. Recently, we examined global miRNA profile of esophageal squamous cell carcinoma (ESCC) demonstrated that miR-92a was highly expressed tissues. In this study, found up-regulation significantly correlated with status lymph node metastasis TNM stage 107 ESCC patients. Moreover, associated poor survival patients might be used as an independent prognostic...
In this study, we investigated whether DNA double-strand breaks (DSBs) contribute to the pathogenesis of chronic obstructive pulmonary disease (COPD). We immunofluorescence-stained lung tissue samples obtained from COPD patients, asymptomatic smokers and nonsmokers for markers DSBs. The numbers DSB foci (phosphorylated histone 2AX (γH2AX), phosphorylated ATM (ataxia telangiectasia mutated) substrate p53-binding protein-1 foci) per cell in alveolar type I II cells endothelial were higher...
Large numbers of maternal RNAs are deposited in oocytes and reserved for later development. Control RNA translation during oocyte maturation has been extensively investigated its regulatory mechanisms well documented. However, translational regulation early oogenesis is largely unexplored. In this study, we generated zebrafish zar1 mutants which result apoptosis fully penetrant male Loss p53 suppresses the restores immature ovaries show upregulation proteins implicated endoplasmic reticulum...
Background Alternative pre-mRNA splicing (AS) plays a central role in generating complex proteomes and influences development disease. However, the regulation etiology of AS human tumorigenesis is not well understood. Methodology/Principal Findings A Basic Local Alignment Search Tool database was constructed for expressed sequence tags (ESTs) from all available databases cancer normal tissues. An insertion or deletion alignment EST/EST used to identify alternatively spliced transcripts. ESTs...
Abstract Bladder cancer (BC) is very common and associated with significant morbidity mortality, though the molecular underpinnings of its origination progression remain poorly understood. In this study, we demonstrate that Prohibitin 1 (PHB) was overexpressed in human BC tissues PHB upregulation poor prognosis. We also found necessary sufficient for cell proliferation. Interestingly, primarily within mitochondria, provide first direct evidence phosphorylation by Akt at Thr258 induces...
Sepsis is a common life-threatening pathology. This study investigated the role of transcription factor sex-determining region Y (SRY)-box 9 (SOX9) in sepsis-induced cardiomyocyte pyroptosis. A murine model sepsis was established, followed by detection cardiac functions and myocardial injury. HL-1 mouse cardiomyocytes were induced lipopolysaccharides (LPS). The levels interleukin (IL)-18, IL-1β, tumor necrosis factor-α (TNF-α), IL-6, malondialdehyde (MDA), superoxide dismutase (SOD) tissues...
Lung cancer remains the most common cause of death for malignancy in both men and women. Current therapies NSCLC patients are inefficient due to lack diagnostic therapeutic markers. The phospho-Ser/Thr-Pro specific prolyl-isomerase Pin1 is overexpressed many different cancers, including NSCLC, may possibly be used as a target therapy. We identified 79 cases with follow-up survival investigated clinical relevance expression patients. To validate oncogenic potential lung cells, we Glc82...
Our previous study found that miR-652-3p is markedly upregulated in the serum of patients with NSCLC and suggesting a potential biomarker for early diagnosis NSCLC. In this study, we detected expression tumor tissues cell lines investigated effect on proliferation metastasis cells. results showed was significantly 50 NSCLC, it higher positive lymph node metastasis, advanced TNM stage poor prognosis. Using functional analyses by overexpressing or suppressing cells, demonstrated promoted...
Colorectal cancer is one of the most common malignancies worldwide. Oxaliplatin first-line chemotherapeutic agent for treatment advanced colorectal cancer. However, acquired resistance to oxaliplatin limits its therapeutic efficacy, and underlying mechanism remains largely unclear. In this study, we compared expression a panel microRNAs (miRNAs) between oxaliplatin-sensitive -resistant HCT-116 cells. We found that miR-454-3p was significantly up-regulated in oxaliplatin-resistant cells...
Abstract Newly diagnosed patients with high-risk acute graft-versus-host disease (aGVHD) often experience poor clinical outcomes and low complete remission rates. Ruxolitinib corticosteroids showed promising efficacy in improving response failure free survival our phase I study. This study (ClinicalTrials.gov: NCT04061876) sought to evaluate the safety effectiveness of combining ruxolitinib (RUX, 5 mg/day) (1 mg/kg/day methylprednisolone, RUX/steroids combined group) versus using...
Abstract This study aimed to identify new pyroptosis-associated tumor antigens for use in mRNA vaccines and the screening of sensitive LUAD populations suitable vaccination. The association between immune infiltrating cell abundance potential was investigated visualized using analysis modules gene expression, clinical outcomes, somatic copy number variation. In addition, pyroptosis-related genes (PRGs) were clustered, relative pyroptosis subtypes (PSs) identified, prognostic value PSs...
Cancer-associated fibroblasts (CAFs) are one of the most predominant cellular subpopulations in tumor stroma and play an integral role cancer occurrence progression. However, prognostic CAFs breast remains poorly understood.
The studies of targeted gene delivery nanocarriers have gained increasing attention during the past decades. In this study, mannan modified DNA loaded bioadhesive PLGA nanoparticles (MAN-DNA-NPs) were investigated for to Kupffer cells (KCs). Bioadhesive prepared and subsequently bound with pEGFP . Following coupling mannan-based PE-grafted ligands (MAN-PE) DNA-NPs, MAN-DNA-NPs delivered intravenously rats. transfection efficiency was determined from isolated KCs flow cytometry applied...
Context: Non-viral gene delivery could deliver drugs/genes through cellular membranes and nuclear by some modification of materials.Objective: This study develops a kind vector to target the cells receptor-mediated pathways. Nuclear localization signal (NLS) was also used increase uptake genetic materials.Materials methods: A lipid containing dexamethasone (Dexa) synthesized as material preparation solid nanoparticles (SLNs) folate (Fa)-conjugated PEG-PE (Fa-PEG-PE) ligands were modify SLNs....
Alternative pre-messenger RNA (mRNA) splicing is a key molecular event that allows for protein diversity and plays important roles in development disease. pre-mRNA regulations during spermatogenesis alternative etiology testicular tumorigenesis are yet to be characterized. By genome-wide analysis, here we describe features distinguish distinctive patterns of among human testis, cancer mouse testis. Through computationally subtractive detected 80 testis-specific transcript candidates 175 262...
Purpose . Biodegradable polymeric nanoparticles have been used frequently as gene delivery vehicles. The aim of this study is to modify bioadhesive PLGA with novel synthetic mannan‐PEG‐PE (MN‐PEG‐PE) obtain active targeted system. Methods Mannan‐PEG‐PE ligands were synthesized and modified onto the NPs/pEGFP complexes. modification rate was optimized, characteristics vehicle evaluated. Then, vectors intravenous delivered rats, in vivo targeting behavior MN‐PEG‐PE nanoparticles/pEGFP...