A5036397909- Algal biology and biofuel production
- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Photoreceptor and optogenetics research
- Micro and Nano Robotics
- Light effects on plants
- Epigenetics and DNA Methylation
- Marine and coastal plant biology
- Microbial Community Ecology and Physiology
- RNA modifications and cancer
- Mercury impact and mitigation studies
- Environmental Toxicology and Ecotoxicology
- Erythrocyte Function and Pathophysiology
- ATP Synthase and ATPases Research
- Genomics and Phylogenetic Studies
- Biomedical Research and Pathophysiology
- Photodynamic Therapy Research Studies
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Nuclear Structure and Function
- Lipid metabolism and biosynthesis
- Cellular Mechanics and Interactions
- Selenium in Biological Systems
Institute of Hydrobiology
2016-2025
Chinese Academy of Sciences
2016-2025
University of Chinese Academy of Sciences
2021-2024
State Key Laboratory of Freshwater Ecology and Biotechnology
2024
Waters (China)
2023
Shandong Normal University
2023
Institute of Hydrobiology, Biology Centre, Academy of Sciences of the Czech Republic
2020
Yale University
2006-2013
National University of Ireland, Maynooth
2009
University of Freiburg
2002-2006
Abstract The National Genomics Data Center (NGDC), which is a part of the China for Bioinformation (CNCB), provides family database resources to support global academic and industrial communities. With rapid accumulation multi-omics data at an unprecedented pace, CNCB-NGDC continuously expands updates core through big archiving, integrative analysis value-added curation. Importantly, NGDC collaborates closely with major international databases initiatives ensure seamless exchange...
Blue light as an environmental cue plays a pivotal role in controlling the progression of sexual life cycle green alga Chlamydomonas reinhardtii . Phototropin was considered prime candidate for blue-light receptor involved. By using RNA interference method, knockdown strains with reduced phototropin levels were isolated. Those severely this photoreceptor partially impaired three steps cycle: gametogenesis, maintenance mating ability, and germination zygotes. These observations suggest that...
To analyze the function of ciliary polycystic kidney disease 2 (PKD2) and its relationship to intraflagellar transport (IFT), we cloned gene encoding Chlamydomonas reinhardtii PKD2 (CrPKD2), a protein with characteristics family members. Three forms this (210, 120, 90 kD) were detected in whole cells; two smaller are cleavage products 210-kD predominant flagella. In cells expressing CrPKD2–GFP, about 10% flagellar CrPKD2–GFP was observed moving membrane. When IFT blocked, fluorescence...
Summary Phototropin (PHOT) is a photoreceptor involved in variety of blue‐light‐elicited physiological processes including phototropism, chloroplast movement and stomatal opening plants. The work presented here tests whether PHOT expression light‐regulated genes Chlamydomonas reinhardtii . When C. was transferred from the dark to very low‐fluence rate white light, there substantial increase level transcripts encoding glutamate‐1‐semialdehyde aminotransferase (GSAT), phytoene desaturase (PDS)...
The disassembly of cilia and flagella is linked to the cell cycle environmental cues. We have found that ubiquitination flagellar proteins an integral part disassembly. Free ubiquitin ubiquitin-conjugating enzyme CrUbc13 are detected in flagella, several ubiquitinated isolated when exogenous adenosine triphosphatase added, suggesting conjugation system operates flagella. Levels increase during resorption, especially intraflagellar transport (IFT) mutants, products labeled with transported...
Abstract Calcium (Ca 2+ ) and redox signalling play important roles in acclimation processes from archaea to eukaryotic organisms. Herein we characterized a unique protein Chlamydomonas reinhardtii that has the competence integrate Ca - redox-related signalling. This protein, designated as calredoxin (CRX), combines four -binding EF-hands thioredoxin (TRX) domain. A crystal structure of CRX, at 1.6 Å resolution, revealed an unusual calmodulin-fold EF-hands, which is functionally linked via...
Precise gene-editing using CRISPR/Cas9 technology remains a long-standing challenge, especially for genes with low expression and no selectable phenotypes in Chlamydomonas reinhardtii, classic model photosynthesis cilia research. Here, we developed multi-type precise genetic manipulation method which DNA break was generated by Cas9 nuclease the repair mediated homologous template. The efficacy of this demonstrated several types gene editing, including inactivation two low-expression (CrTET1...
In migratory fibroblasts, the front-rear polarity required for cell migration is defined by an anterior centrosome relative to nucleus. To achieve this polarity, actin cables drive nucleus backward coupling nuclear membrane proteins nesprin-2G and SUN2. Aging disrupts increasing protein levels of SUN1, a SUN2 homolog. Here, we investigated molecular mechanisms behind disruption found that dominant negative effect SUN1 progerin, aging-related lamin A variant, direct SUN1-lamin interaction....
Cilia and flagella associated protein 410 (CFAP410) is a localized at the basal body of cilia/flagella plays essential roles in ciliogenesis. Multiple single amino acid mutations CFAP410 have been identified patients. However, molecular mechanism for how cause these disorders remains poorly understood due to lack high-resolution structures protein. Our studies demonstrate that adopts bimodular architecture. We previously reported our structural on C-terminal domain (CTD) from various...
In the search for a Chlamydomonas reinhardtii photoreceptor that may mediate blue-light-induced responses we identified gene encodes protein with structure typical of members phototropin family, i.e. two LOV domains function in flavin mononucleotide binding and ser/thr kinase domain. The amino acid sequences these are closely related to those higher plant phototropins. This single-copy (Phot) calculated molecular mass 81.4 kDa which is distinctly smaller than homologous proteins plants...
The unicellular green alga, Chlamydomonas reinhardtii, is a classic model for studying flagella and biofuel. However, precise gene editing, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) CRISPR-associated protein (Cas9) system, not widely used in this organism. Screening of random insertional mutant libraries by polymerase chain reaction provides an alternate strategy to obtain null mutants individual gene. But building, screening, maintaining library was...
Cilia are microtubule-based organelles and perform motile, sensing signaling functions. The assembly maintenance of cilia depend on intraflagellar transport (IFT). Besides ciliary localization, most IFT proteins accumulate at basal bodies. However, little is known about the molecular mechanism body targeting proteins. We first identified possible body-targeting sequence in IFT46 by expressing truncation constructs an ift46-1 mutant. C-terminal between residues 246-321, termed BBTS3, was...
Cilia/flagella are structurally conserved and dynamic organelles; their assembly disassembly coordinated with the cell cycle differentiation. Several post-translational modifications, including acetylation, methylation, phosphorylation ubiquitylation, participate in ciliary disassembly. However, detailed mechanism role of ubiquitylation unclear. This study identified 20 proteins that were ubiquitylated shortening flagella Chlamydomonas α-Tubulin was most abundant protein it labeled K63...
Abstract Hexavalent chromium [Cr(Ⅵ)] is a highly toxic contaminant in aquatic systems, and microalgae represent promising bioremediators of metal‐containing wastewater. However, the metal‐binding capacity algal cells limited. Therefore, we improved cellular Cr(Ⅵ) biosorption Chlamydomonas reinhardtii by overexpressing sulfate transporter gene SULTR2 . was predominantly located cytoplasm cell, few proteins mobilized to cell membrane as Cr under stress conditions. Intracellular accumulation...
Summary The unicellular green algae C hlamydomonas reinhardtii is a classic model for the study of flagella/cilia and photosynthesis, it has recently been exploited producing biopharmaceuticals biofuel. Due to low frequency homologous recombination, reverse genetic manipulation in relies mainly on mi RNA ‐ si ‐based knockdown methods. However, difficulty constructing artificial vectors, laborious screening transformants, undesired epigenetic silencing exogenous constructs limit their...
Abstract DMC1 is a recombinase that essential for meiotic synapsis. Experiments in extensive species of eukaryotes have indicated the independent role repairing double strand breaks (DSBs) produced during meiosis I. Mutation dmc1 mice and human often leads to obstacles spermatogenesis male sterility. Here, we report on disruption medaka ( Oryzias latipes ). Synapsis was disturbed mutant testis nuclei, as observed other organisms. Unexpectedly, could produce few sperm and, although most these...
Abstract Lysosome-related organelles (LROs) are a class of heterogeneous conserved in eukaryotes that primarily play role storage and secretion. An important function LROs is to mediate metal homeostasis. Chlamydomonas reinhardtii model organism for studying ion metabolism; however, structural functional analyses C. insufficient. Here, we optimized method purifying these from 2 populations cells: stationary phase or overloaded with iron. The morphology, elemental content, lysosomal...
Blue light controls the sexual life cycle of Chlamydomonas, mediated by phototropin, a UV-A/blue-light receptor that plays prominent role in multiple photoresponses. By using fractionation experiments and immunolocalization studies, this blue-light receptor, addition to its known localization cell bodies, also was detected flagella. Within flagella, it completely associated with axonemes, striking contrast situation higher plants Chlamydomonas body where phototropin observed plasma membrane....
Hermansky-Pudlak syndrome (HPS) is a human autosomal recessive disorder that characterized by oculocutaneous albinism and deficiency of the platelet storage pool resulting from defective biogenesis lysosome-related organelles (LROs). To date, 10 HPS genes have been identified, three which belong to octamer complex BLOC-1 (biogenesis 1). One subunit complex, BLOS1, also participates in BLOC-1-related (BORC). Due lethality at early embryo stage BLOS1 knockout mice, function above two complexes...