A5029242864- Methane Hydrates and Related Phenomena
- Geophysics and Gravity Measurements
- Ionosphere and magnetosphere dynamics
- Seismic Waves and Analysis
- Earthquake Detection and Analysis
- Seismology and Earthquake Studies
- Algal biology and biofuel production
- Trace Elements in Health
- Photosynthetic Processes and Mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Neurological diseases and metabolism
- Hemoglobin structure and function
- Prion Diseases and Protein Misfolding
- Physiological and biochemical adaptations
- Escherichia coli research studies
- Plant Stress Responses and Tolerance
- Amino Acid Enzymes and Metabolism
- Porphyrin Metabolism and Disorders
- Biomedical Research and Pathophysiology
- Neonatal Health and Biochemistry
- Aquatic Ecosystems and Phytoplankton Dynamics
- Erythrocyte Function and Pathophysiology
- Geochemistry and Elemental Analysis
- Bacterial Genetics and Biotechnology
University of California, Los Angeles
2021-2024
University of California, Berkeley
2021-2023
QB3
2021-2023
Lawrence Livermore National Laboratory
2021-2023
Oregon Health & Science University
2016-2017
Environmental Health
2017
United States Department of Agriculture
2013-2016
Western Regional Research Center
2013-2016
Agricultural Research Service
2016
Significance Transition metals are of crucial importance for primary productivity; their scarcity limits crop yield in agriculture and carbon sequestration on a global scale. Copper (Cu), iron (Fe), manganese (Mn) among the most important trace elements that enable redox chemistry oxygenic photosynthesis. The single-celled, eukaryotic green alga Chlamydomonas reinhardtii is choice experimental system studying metal homeostasis context phototrophy, offering all advantages classical microbial...
Abstract Lysosome-related organelles (LROs) are a class of heterogeneous conserved in eukaryotes that primarily play role storage and secretion. An important function LROs is to mediate metal homeostasis. Chlamydomonas reinhardtii model organism for studying ion metabolism; however, structural functional analyses C. insufficient. Here, we optimized method purifying these from 2 populations cells: stationary phase or overloaded with iron. The morphology, elemental content, lysosomal...
SUMMARY Even subtle modifications in growth conditions elicit acclimation responses affecting the molecular and elemental makeup of organisms, both laboratory natural habitats. We systematically explored effect temperature, pH, nutrient availability, culture density, access to CO 2 O laboratory‐grown algal cultures on rate, ionome, ability accumulate Fe. found cells Fe alkaline conditions, even more so when excess is present, coinciding with a reduced rate. Using combination Fe‐specific...
We employed a sensitive mass spectrometry-based method to deconstruct, confirm, and quantitate the prions present in elk naturally infected with chronic wasting disease sheep scrapie. used this approach study oxidation of methionine at position 216 (Met216), because (MetSO216) has been implicated prion formation. Three polymorphisms (Ile218, Val218, Thr218) recombinant protein were prepared. Our analysis showed novel result that proportion MetSO216 was highly dependent upon amino acid...
A heme-dependent conformational rearrangement of the C-terminal domain heme binding protein (PhuS) is required for interaction with iron-regulated oxygenase (HemO). Herein, we further investigate underlying mechanism this and its implications transfer via site-directed mutagenesis, resonance Raman (RR), hydrogen-deuterium exchange MS (HDX-MS) methods, molecular dynamics (MD). HDX-MS revealed that apo-PhuS α6/α7/α8-helices are largely unstructured, whereas H212R variant showed an increase in...
Shiga-like toxins (verotoxins) are a class of AB5 holotoxins that primarily responsible for the virulence associated with toxin producing Escherichia coli (STEC) infections. The composed pentamer identical subunits (B subunit) delivering catalytic subunit (A to host cell and facilitating endocytosis into cell. B not toxicity. We developed multiple reaction monitoring method based on analyzing conserved peptides, derived from tryptic digestion subunits. Stable-isotope-labeled analogues were...
Scrapie is a prion (PrPSc) disease of sheep. The incubation period sheep scrapie strongly influenced by polymorphisms at positions 136, 154, and 171 sheep's normal cellular protein (PrPC). Chymotrypsin was used to digest recombinant PrP identify set characteristic peptides [M132LGSXMSRPL141 (X = A or V), Y153XENMY158 (X,= H R), Y166RPVDXY172 H, K, Q, R)] that could be detect quantitate PrPC PrPSc. These were develop multiple reaction monitoring method (MRM) the amounts particular...