A5084941521- Cellular Mechanics and Interactions
- Nuclear Structure and Function
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Skin and Cellular Biology Research
- Axon Guidance and Neuronal Signaling
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Neurogenesis and neuroplasticity mechanisms
- Advanced Fluorescence Microscopy Techniques
- Cellular transport and secretion
- Congenital limb and hand anomalies
Columbia University
2015-2025
Columbia College
2023
Royal College of Physicians
2023
Fascin is an F-actin-bundling protein shown to stabilize filopodia and regulate adhesion dynamics in migrating cells, its expression correlated with poor prognosis increased metastatic potential a number of cancers. Here, we identified the nuclear envelope nesprin-2 as binding partner for fascin range cell types vitro vivo. Nesprin-2 interacts through direct, F-actin-independent interaction, this distinct separable from role within at periphery. Moreover, disrupting interaction between...
In migratory fibroblasts, the front-rear polarity required for cell migration is defined by an anterior centrosome relative to nucleus. To achieve this polarity, actin cables drive nucleus backward coupling nuclear membrane proteins nesprin-2G and SUN2. Aging disrupts increasing protein levels of SUN1, a SUN2 homolog. Here, we investigated molecular mechanisms behind disruption found that dominant negative effect SUN1 progerin, aging-related lamin A variant, direct SUN1-lamin interaction....
B-cell receptor (BCR) engagement with surface-tethered antigens leads to the formation of an immune synapse, which facilitates antigen uptake for presentation T-lymphocytes. Antigen internalization and processing rely on early dynein-dependent transport BCR-antigen microclusters synapse center, as well later polarization microtubule-organizing center (MTOC). MTOC repositioning allows release proteases delivery MHC class II molecules at synapse. Whether how these events are coordinated have...
ABSTRACT RhoA plays a crucial role in neuronal polarization, where its action restraining axon outgrowth has been thoroughly studied. We now report that not only an inhibitory but also stimulatory effect on development depending when and exerts the downstream effectors involved. In cultured hippocampal neurons, FRET imaging revealed activity selectively localized growth cones of undifferentiated neurites, whereas developing axons it displayed biphasic pattern, being low nascent high...
Abstract The recycling of integrin endocytosed during focal adhesion (FA) disassembly is critical for cell migration and contributes to the polarized formation new FAs toward leading edge. How this occurs unclear. Here, we sought identify kinesin motor protein(s) that involved in back plasma membrane. We show kinesin-2 heterodimer, KIF3AC Rab11 adaptor protein RCP are required FA reformation after mouse human fibroblasts. In absence KIF3AC, does not return surface found endocytic...
Abstract RhoA plays a crucial role in neuronal polarization, where its action restraining axon outgrowth has been thoroughly studied. We now report that not only inhibitory but also stimulatory effect on development depending when and exerts the downstream effectors involved. In cultured hippocampal neurons, FRET imaging revealed activity selectively localizes growth cones of undifferentiated neurites, while developing axons it displays biphasic pattern, being low nascent high elongating...
Abstract The ability of a cell to regulate its mechanical properties is central function. Emerging evidence suggests that interactions between the nucleus and cytoskeleton influence mechanics through poorly understood mechanisms. Here we show A- B-type nuclear lamin isoforms distinctively modulate both cellular volume selectively stabilize linker nucleoskeleton (LINC) complexes couple cytoskeletal actin vimentin. We reveal, further, loss each four-known in mouse embryonic fibroblasts...