A5068567131- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- Neurobiology and Insect Physiology Research
- Cancer Treatment and Pharmacology
- Microtubule and mitosis dynamics
- Health and Medical Research Impacts
- Silk-based biomaterials and applications
- Estrogen and related hormone effects
- Cell Adhesion Molecules Research
- Mitochondrial Function and Pathology
- Nuclear Structure and Function
- Diversity and Career in Medicine
- 14-3-3 protein interactions
- Genetics, Bioinformatics, and Biomedical Research
- Healthcare and Venom Research
- Force Microscopy Techniques and Applications
- Histone Deacetylase Inhibitors Research
- Cancer-related cognitive impairment studies
- Innovative Teaching Methods
- Biomedical and Engineering Education
- Advanced Fluorescence Microscopy Techniques
- Steroid Chemistry and Biochemistry
- Engineering Education and Pedagogy
- Innovations in Medical Education
- Biochemical effects in animals
Mayo Clinic
2017-2024
University of Iowa
2012-2019
Carver Bible College
2019
Mayo Clinic in Arizona
2017-2018
WinnMed
2017
University of Bristol
2012
Fascin is an F-actin-bundling protein shown to stabilize filopodia and regulate adhesion dynamics in migrating cells, its expression correlated with poor prognosis increased metastatic potential a number of cancers. Here, we identified the nuclear envelope nesprin-2 as binding partner for fascin range cell types vitro vivo. Nesprin-2 interacts through direct, F-actin-independent interaction, this distinct separable from role within at periphery. Moreover, disrupting interaction between...
Although prostaglandins (PGs)—lipid signals produced downstream of cyclooxygenase (COX) enzymes—regulate actin cytoskeletal dynamics, their mechanisms action are unknown. We previously established Drosophila oogenesis, in particular nurse cell dumping, as a new model to determine how PGs regulate remodeling. PGs, and thus the COX-like enzyme Pxt, required for both parallel filament bundle formation cortical strengthening dumping. Here we provide first link between Fascin (Drosophila Singed,...
Drosophila oogenesis provides a developmental system with which to study nuclear actin. During Stages 5-9, actin levels are high in the oocyte and exhibit variation within nurse cells. Cofilin Profilin, regulate import export of actin, also localize nuclei. Expression GFP-tagged Actin results rod formation. These findings indicate that must be tightly regulated during oogenesis. One factor mediating this regulation is Fascin. Overexpression Fascin enhances GFP-Actin formation, colocalizes...
Chemotherapy-induced peripheral neuropathy (CIPN) is a prevalent side effect of widely used platinum-based anticancer agents. There are few predictable risk factors with which to identify susceptible patients. Effective preventive measures or treatments not available. Here, we have model CIPN in Drosophila melanogaster genetic changes that confer resistance cisplatin-induced neuronal damage but the rapidly dividing cells ovary. The strain attP40, as background for creation RNAi lines,...
Fascin, a highly conserved actin-bundling protein, localizes and functions at new cellular sites in both Drosophila multiple mammalian cell types. During follicle development, addition to being cytoplasmic, Fascin is the nuclei of germline-derived nurse cells during stages 10B–12 (S10B–12) nuclear periphery stage 13 (S13). This localization specific as other actin-binding proteins, Villin Profilin, do not exhibit same subcellular distribution. In addition, fascin1 nucleus observed Thus...
Staff from the Mayo Clinic in US and Karolinska Institute Sweden describe a joint transatlantic course intended to broaden horizons of next generation researchers field regenerative medicine.
Prostaglandins (PGs) are lipid signaling molecules with numerous physiologic functions, including pain/inflammation, fertility, and cancer. PGs produced downstream of cyclooxygenase (COX) enzymes, the targets non-steroidal anti-inflammatory drugs (NSAIDs). In systems, regulate actin cytoskeletal remodeling, however, their mechanisms action remain largely unknown. To address this deficiency, we undertook a pharmaco-genetic interaction screen during late-stage Drosophila oogenesis. oogenesis...
Drosophila melanogaster has recently been developed as a simple, in vivo, genetic model of chemotherapy-induced peripheral neuropathy. Flies treated with the chemotherapy agent cisplatin display both neurodegenerative phenotype and cell death rapidly dividing follicles, mimicking specific responses seen humans. Cisplatin induces climbing deficiencies loss fertility dose dependent manner. sensitivity to types is affected by background. We show that mutation or RNAi-based knockdown genes known...
Analysis of mitochondrial function has broad applicability in many research specialties. Neurodegenerative disorders such as chemotherapy-induced peripheral neuropathy (CIPN) often exhibit damaged mitochondria or reduced respiratory capacity. Isolation intact for protein analysis respiration measurements been previously reported numerous model organisms. Here, we describe an adaptation previous protocols to isolate functional from Drosophila melanogaster use a CIPN. Whole are ground...
Over 40 years of controversy surround the nuclear localization actin. Now, it is well understood that actin to nucleus highly regulated, suggesting has critical functions. Studies have demonstrated roles in regulating transcription, chromatin remodeling, organization, and DNA damage repair. However, much remains unknown about actin, including its structure functions during development. Here we present our novel finding Drosophila oogenesis, or follicle development, a model for studying...
Abstract Prostaglandins (PGs) are lipid signaling molecules with numerous physiologic functions, including pain/inflammation, fertility, and cancer. PGs produced downstream of cyclooxygenase (COX) enzymes, the targets non-steroidal anti-inflammatory drugs (NSAIDs). In systems, regulate actin cytoskeletal remodeling, however, their mechanisms action remain largely unknown. To address this deficiency, we undertook a pharmaco-genetic interaction screen during late-stage Drosophila oogenesis....