A5025911801- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Neurobiology and Insect Physiology Research
- Genetic diversity and population structure
- Skin and Cellular Biology Research
- RNA modifications and cancer
- Force Microscopy Techniques and Applications
- Estrogen and related hormone effects
- Hippo pathway signaling and YAP/TAZ
- Genetic Mapping and Diversity in Plants and Animals
- Microtubule and mitosis dynamics
- Genetic and phenotypic traits in livestock
- Identification and Quantification in Food
- Bat Biology and Ecology Studies
- Invertebrate Immune Response Mechanisms
- Coronary Artery Anomalies
- 3D Printing in Biomedical Research
- Cancer-related molecular mechanisms research
- Amphibian and Reptile Biology
- Genomics and Chromatin Dynamics
- Circular RNAs in diseases
- Cardiac Valve Diseases and Treatments
- Congenital heart defects research
- Cardiomyopathy and Myosin Studies
Stowers Institute for Medical Research
2022-2025
University of Iowa
2017-2021
Carver Bible College
2019
Purdue University West Lafayette
2014-2017
The goal of captive breeding programmes is often to maintain genetic diversity until re-introductions can occur. However, due in part changes that occur populations, approximately one-third fail. We evaluated populations using microsatellites and mtDNA. analysed six white-footed mice were propagated for 20 generations two replicates three protocols: random mating (RAN), minimizing mean kinship (MK) selection docility (DOC). found MK resulted the slowest loss microsatellite compared RAN DOC....
Abstract Neural crest cells (NCC) are a migratory progenitor cell population considered unique to vertebrates. Derived from the neuroepithelium during early embryogenesis, NCC contribute nearly every tissue and organ system throughout body, disruptions in development can result congenital disorders, termed neurocristopathies. Despite decades of research, we have poor understanding cellular mechanisms signals that govern mammalian formation. We discovered nuclear receptor superfamily 6 group...
A key regulator of collective cell migrations, which drive development and cancer metastasis, is substrate stiffness. Increased stiffness promotes migration controlled by Myosin. Using Drosophila border as a model migration, we identify, for the first time, that actin bundling protein Fascin limits Myosin activity in vivo. Loss results in: increased activated on cells their substrate, nurse cells; decreased dynamics; measured atomic force microscopy. Reducing restores on-time fascin mutant...
Transcription of ribosomal RNA (rRNA) by Polymerase (Pol) I in the nucleolus is necessary for ribosome biogenesis, which intimately tied to cell growth and proliferation. Perturbation biogenesis results tissue specific disorders termed ribosomopathies association with alterations nucleolar structure. However, how rRNA transcription regulate structure during normal development pathogenesis disease remains poorly understood. Here we show that homozygous null mutations Pol subunits required...
The inability to effectively stimulate cardiomyocyte proliferation remains a principle barrier regeneration in the adult human heart. A tightly regulated, acute inflammatory response mediated by range of cell types is required initiate regenerative processes. Prostaglandin E
The actin bundling protein Fascin is essential for developmental cell migrations and promotes cancer metastasis. In addition to actin, has several actin-independent roles; how these other functions contribute migration remains unclear. Border during Drosophila oogenesis provides an excellent model study Fascin's various roles invasive, collective migration.On-time border Stage 9 requires (Drosophila Singed). not only within the migrating cells, but also nurse substrate this migration....
Prostaglandins (PGs) are lipid signaling molecules with numerous physiologic functions, including pain/inflammation, fertility, and cancer. PGs produced downstream of cyclooxygenase (COX) enzymes, the targets non-steroidal anti-inflammatory drugs (NSAIDs). In systems, regulate actin cytoskeletal remodeling, however, their mechanisms action remain largely unknown. To address this deficiency, we undertook a pharmaco-genetic interaction screen during late-stage Drosophila oogenesis. oogenesis...
While prostaglandins (PGs), short-range lipid signals, regulate single cell migration, their roles in collective migration remain unclear. To address this, we use Drosophila border an invasive, that occurs during Stage 9 of oogenesis. Pxt is the cyclooxygenase-like enzyme responsible for PG synthesis. Loss results both delayed and elongated clusters, whereas somatic knockdown causes compacted clusters. These findings suggest PGs act cells nurse cells, substrate on which migrate. As actin...
Genetic diversity (GD) is largely determined by effective population size, which may vary dramatically for species that differ in key aspects of their biology such as vagility. To identify evolutionary patterns associated with animal distributions and movements, we examined relationships among GD (i.e. microsatellite heterozygosity allelic richness), taxonomic class, biome migratory behaviour. Linear regression revealed mammals, reptiles, amphibians fishes had less compared to nonmigratory...
Fascin is an actin bundling protein that regulates dynamics of cytoskeletal structures, such as filopodia and invadopodia, during cell migration. Importantly, known to mediate retinal formation axonal growth development by promoting In addition, recent research has demonstrated plays a significant role in cancer invasion metastasis. However, additional roles Fascin, including regulation microtubules, interaction with mechanotransduction machinery, nuclear localization, have been uncovered...
Abstract Fascin is an actin bundling protein that essential for developmental cell migrations and promotes cancer metastasis. In addition to actin, has several actin-independent roles. Border migration during Drosophila oogenesis provides excellent model study Fascin’s various roles invasive, collective migration. requires Fascin. functions not only within the migrating border cells, but also nurse substrate this Loss of results in increased, shorter mislocalized protrusions Data supports...
Abstract While prostaglandins (PGs), short-range lipid signals, regulate cell migration, their mechanisms of action are poorly understood in collective migration. To address this, we use Drosophila border migration during Stage 9 oogenesis. The cells delaminate from the epithelium, and migrate collectively invasively between nurse cells. Pxt is cyclooxygenase-like enzyme responsible for all PG synthesis. Loss results both a significant delay an increase cluster length compared to wild-type...
Summary Beginning with transcription of ribosomal RNA (rRNA) by Polymerase (Pol) I in the nucleolus, ribosome biogenesis is intimately tied to cell growth and proliferation. Perturbation has been previously shown affect nucleolar structure, yet underlying mechanism unknown. We generated loss-of-function mouse mutants Pol subunits, Polr1a, Polr1b, Polr1c Polr1d , thereby genetically inhibiting rRNA biogenesis. mutant embryos are preimplantation lethal have fewer nucleoli. Using hiPSCs triple...
Prostaglandins (PGs) are lipid signals that play critical roles in physiology, including regulating pain and inflammation, reproduction, cancer. However, the cellular mechanisms of PG action remain poorly understood. PGs synthesized downstream cyclooxygenase (COX) enzymes. In Drosophila, there is one COX‐like enzyme Pxt. Loss synthesis both mammals flies results female sterility. Specifically, loss Pxt causes numerous defects oogenesis, i.e. follicle development. One mechanism whereby...
Abstract Prostaglandins (PGs) are lipid signaling molecules with numerous physiologic functions, including pain/inflammation, fertility, and cancer. PGs produced downstream of cyclooxygenase (COX) enzymes, the targets non-steroidal anti-inflammatory drugs (NSAIDs). In systems, regulate actin cytoskeletal remodeling, however, their mechanisms action remain largely unknown. To address this deficiency, we undertook a pharmaco-genetic interaction screen during late-stage Drosophila oogenesis....
Abstract A key regulator of collective cell migrations, which drive development and cancer metastasis, is substrate stiffness. Increased stiffness promotes migration controlled by Myosin. Using Drosophila border as a model migration, we identify, for the first time, that actin bundling protein Fascin limits Myosin activity in vivo . Loss results in: increased activated on cells their substrate, nurse cells; decreased dynamics; measured atomic force microscopy. Reducing restores on-time...