A5102821181- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Developmental Biology and Gene Regulation
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Asymmetric Hydrogenation and Catalysis
- RNA modifications and cancer
- Corneal Surgery and Treatments
- Congenital heart defects research
- Organometallic Complex Synthesis and Catalysis
- Neonatal Respiratory Health Research
- Paleontology and Evolutionary Biology
- Cancer-related gene regulation
- Peroxisome Proliferator-Activated Receptors
- Plant Reproductive Biology
- Molecular Biology Techniques and Applications
- Cholesterol and Lipid Metabolism
- Genetic and Environmental Crop Studies
- Insect Resistance and Genetics
- interferon and immune responses
- Histone Deacetylase Inhibitors Research
- Single-cell and spatial transcriptomics
- Drug Transport and Resistance Mechanisms
- Circular RNAs in diseases
- Proteoglycans and glycosaminoglycans research
Stowers Institute for Medical Research
2020-2025
University of Virginia
2018-2021
Monklands Hospital
2016
Cardiff University
2000-2005
Cardiff Metropolitan University
2001-2002
University of Wales
2002
National Renewable Energy Laboratory
2002
University of Colorado Boulder
2002
University of Wales Institute Cardiff
2000
5-Methylcytosine (m5C) is a RNA modification that exists in tRNAs and rRNAs was recently found mRNAs. Although it has been suggested to regulate diverse biological functions, whether m5C influences adult stem cell development remains undetermined. In this study, we show Ypsilon schachtel (YPS), homolog of human Y box binding protein 1 (YBX1), promotes germ line (GSC) maintenance, proliferation, differentiation the Drosophila ovary by preferentially m5C-containing RNAs. YPS genetically...
Post-translational modifications of histone tails play a crucial role in gene regulation. Here, we performed chromatin profiling by quantitative targeted mass spectrometry to assess all possible the core histones. We identified bivalent combination, dually marked H3K9me3/H3K14ac modification liver, that is significantly decreased old hepatocytes. Subsequent sequential ChIP-Seq single nucleosome regions, with reduced number sites and signal livers, confirming results. detected H3K9me3 H3K14ac...
Abstract Neural crest cells (NCC) are a migratory progenitor cell population considered unique to vertebrates. Derived from the neuroepithelium during early embryogenesis, NCC contribute nearly every tissue and organ system throughout body, disruptions in development can result congenital disorders, termed neurocristopathies. Despite decades of research, we have poor understanding cellular mechanisms signals that govern mammalian formation. We discovered nuclear receptor superfamily 6 group...
We present a SNP-based crossover map for Drosophila mauritiana. Using females derived by crossing two different strains of D. mauritiana, we analyzed over on all five major chromosome arms. Analysis 105 male progeny allowed us to identify 327 chromatids bearing single, double, or triple events, representing 398 events. mapped the crossovers along these arms using genome sequence that includes euchromatin-heterochromatin boundary. Confirming previous studies, show overall frequency in...
The Rh(I) and Rh(III) hydrides HRh(dppb)2 [HRh(dppb)2(NCCH3)](BF4)2 (where dppb is 1,2-(bis(diphenylphosphino)benzene) have been prepared, a structural study of has completed. latter complex an octahedral with trans arrangement the hydride acetonitrile ligands. A pKa value 9.4 was measured for this by equilibration [Rh(dppb)2](BF4) 4-bromoanilinium tetrafluoroborate in acetonitrile. reacts H2 presence Pt(dmpp)2, which acts as base, to form [HPt(dmpp)2](BF4) dmpp =...
Summary Increasing evidence suggests that regulation of heterochromatin at the nuclear envelope underlies metabolic disease susceptibility and age‐dependent changes, but mechanism is unknown. Here, we profile lamina‐associated domains ( LAD s) using lamin B1 Ch IP ‐Seq in young old hepatocytes find that, although resides a large fraction both ages, third B1‐associated regions are bound exclusively each age vivo. Regions occupied by solely livers enriched for forkhead motif, Foxa pioneer...
Abstract Retinoic acid (RA) is involved in antero-posterior patterning of the chordate body axis and, jawed vertebrates, has been shown to play a major role at multiple levels gene regulatory network (GRN) regulating hindbrain segmentation. Knowing when and how RA became coupled core GRN important for understanding ancient signaling pathways genes can evolve generate diversity. Hence, we investigated link between segmentation sea lamprey Petromyzon marinus , an jawless vertebrate model...
Type II nuclear hormone receptors, including farnesoid X receptors (FXR), liver (LXR), and peroxisome proliferator-activated (PPAR), which serve as drug targets for metabolic diseases, are permanently positioned in the nucleus thought to be bound DNA regardless of ligand status. However, recent genome-wide location analysis showed that LXRα PPARα binding is largely ligand-dependent. We hypothesized pioneer factor Foxa2 evicts nucleosomes enable ligand-dependent type performed studies test...
Neurocristopathies such as CHARGE syndrome result from aberrant neural crest development. A large proportion of cases are attributed to pathogenic variants in the gene encoding CHD7, chromodomain helicase DNA binding protein 7, which remodels chromatin. While role for CHD7 development is well documented, how this factor specifically up-regulated cells not understood. Here, we use epigenomic profiling chick and human identify a cohort enhancers regulating Chd7 expression other tissues. We...
SUMMARY Phosphatidylcholine (PC) is the major phospholipid of pulmonary surfactant and it hypothesized that PC its subspecies modulate functions alveolar macrophages. The most abundant these dipalmitoylphosphatidylcholine (DPPC). This study was undertaken to determine effect on monocyte function using a human monocytic cell line, MonoMac-6 (MM6). showed preincubation MM6 cells with DPPC at 125 μg/ml for 2 h inhibited oxidative response either zymosan or phorbol-12-myristate-13-acetate (PMA)...
Meiosis is usually described as 4 essential and sequential processes: (1) homolog pairing; (2) synapsis, mediated by the synaptonemal complex; (3) crossing over; (4) segregation. In this canonical model, maturation of crossovers into chiasmata plays a vital role in holding homologs together ensuring their segregation at first meiotic division. However, Lepidoptera (moths butterflies) undergo 3 distinct processes, only one which canonical. males utilize 2 meiosis that produces nucleated...
Abstract In eukaryotes, ATP-dependent chromatin remodelers regulate gene expression in response to nutritional and metabolic stimuli. However, altered transcription of genes may have significant indirect consequences which are currently poorly understood. this study, we use genetic molecular approaches uncover a role for the remodeler Swi-Snf as critical regulator metabolism. We find that snfΔ mutants display cysteine-deficient phenotype, despite growth nutrient-rich media. This correlates...
A new template synthesis of triphosphorus macrocycles has been developed based upon the intramolecular hydrophosphination allylphosphine coordinated to (η5-cyclopentadienyl)iron(II) cation; resulting tri-secondary macrocycle 1,5,9-triphosphacyclododecane is readily alkylated with ethene give (η3-1,5,9-[12]aneP3Et3)Fe(η5 -Cp)+ which in turn liberated as free ligand stereospecifically syn–syn isomer; related reactions phenyl(allyl)phosphine lead directly triphenyl (1,5,9-[12]aneP3Ph3) also...
Primary and secondary phosphine piano-stool complexes of the type [eta(5)-CpFeL3]+ (L = phenylphosphine, 3, (alpha-methyl)vinylphosphine, 4, allylphosphine, 5, (2-methylpropenyl)phosphine, 5b, allyl(phenyl)phosphine, 6) are described. The alkenyl complexes, 5 6, react by intramolecular hydrophosphination to give corresponding [eta(5)-CpFe]+ 1,5,9-triphosphacyclododecane (12-aneP3R3, 2, R H), 9 10 respectively. Alkylation phosphines in is achieved hydrophosphinations with ethene 12-aneP3R3 (R...
Abstract Glaucoma is the leading cause of irreversible blindness with trabecular meshwork (TM) dysfunction resulting in elevated intraocular pressure and retinal ganglion cell (RGC) damage to vision loss. In this study, we discovered that secretome, derived from human TM stem cells, via minimal invasive periocular injection, can reduce pressure, restore homeostasis, protect RGC, RGC function both steroid-induced genetic myocilin mutant mouse models glaucoma. The secretome upregulated...
The ancestral mode of left-right (L-R) patterning involves cilia in the L-R organizer. However, mechanisms regulating non-avian reptiles remains an enigma, since most squamate embryos are undergoing organogenesis at oviposition. In contrast, veiled chameleon (Chamaeleo calyptratus) pre-gastrula oviposition, making them excellent organism for studying evolution. Here we show that lack motile time asymmetry establishment. Thus, loss organizers is a synapomorphy all reptiles. Furthermore,...
Infective endocarditis is an important cause of morbidity and mortality in patients receiving haemodialysis for established renal failure. We carried out a prospective audit developing infective single unit.From 1 January 2000 to 31 December 2013, we collected data on all cases occurring at Monklands Hospital, Airdrie. Twenty-nine developed during our period. Twenty-three (79.3%) the had pre-existing cardiac valve abnormalities such as regurgitation or calcification. Staphylococcus aureus...
Abstract Corneal scarring is a leading cause of blindness in the world. In this study, we explored therapeutic potential corneal stromal stem cell (CSSC)-derived secretome mechanical debridement mouse model scarring. CSSC was able to promote scarless wound healing. The mechanisms include 1) dampening inflammation with reduced CD45+, CD11b+/GR1+, and CD11b+/F4/80+ inflammatory cells wounded corneas; 2) reducing fibrotic extracellular matrix deposition such as collagen IV, 3A1, SPARC, α-SMA;...
Abstract A properly regulated series of developmental and meiotic events must occur to ensure the successful production gametes. In Drosophila melanogaster ovaries, these early include 16-cell cyst, entry, synaptonemal complex (SC) formation, recombination, oocyte specification. order identify additional genes involved in development meiosis, we reanalyzed 3 published single-cell RNA-seq datasets from using vasa (germline) together with c(3)G, cona, corolla as markers. Our analysis generated...
The family Chamaeleonidae comprises 228 species, boasting an extensive geographic spread and array of evolutionary novelties adaptations, but a paucity genetic molecular analyses. Veiled chameleon (
Abstract We present a SNP-based crossover map for Drosophila mauritiana . Using females derived by crossing two different strains of D , we analyzed over on all five major chromosome arms. Analysis 105 male progeny allowed us to identify 327 chromatids bearing single, double, or triple events, representing 398 separate events. mapped these sites along arms using genome sequence that includes the euchromatin-heterochromatin boundary. Confirming previous studies, show overall frequency in is...
ABSTRACT The ancestral mode of left-right (L-R) patterning involves cilia in the L-R organizer. However, mechanisms regulating non-avian reptiles remains an enigma, since most squamate embryos are undergoing organogenesis at oviposition. In contrast, veiled chameleon ( Chamaeleo calyptratus ) pre-gastrula oviposition, making them excellent organism for studying evolution. Here we show that lack motile their organizer, consistent with loss being a synapomorphy all reptiles. Furthermore,...