A5056670181- Malaria Research and Control
- Mosquito-borne diseases and control
- Complement system in diseases
- Drug Transport and Resistance Mechanisms
- HIV Research and Treatment
- Invertebrate Immune Response Mechanisms
- vaccines and immunoinformatics approaches
- Trypanosoma species research and implications
- Aquaculture disease management and microbiota
- Computational Drug Discovery Methods
- Research on Leishmaniasis Studies
- Monoclonal and Polyclonal Antibodies Research
- Vector-borne infectious diseases
- Hemoglobinopathies and Related Disorders
- Parasites and Host Interactions
- Erythrocyte Function and Pathophysiology
- Insect Resistance and Genetics
- Lipid Membrane Structure and Behavior
- Vibrio bacteria research studies
- Hepatitis B Virus Studies
- Hepatitis Viruses Studies and Epidemiology
- Venomous Animal Envenomation and Studies
- Insect symbiosis and bacterial influences
- Toxoplasma gondii Research Studies
- Blood groups and transfusion
National Institute of Allergy and Infectious Diseases
2015-2024
National Institutes of Health
2012-2022
Hofstra University
2021-2022
Vector (United States)
2013-2018
New York University
2014-2015
VA NY Harbor Healthcare System
2012-2015
Vector Oncology (United States)
2004-2014
NYU Langone Health
2011
Virginia Commonwealth University
2009
University of Bamako
2001-2007
Duffy-blood-group-negative human erythrocytes, FyFy, are resistant to invasion in vitro by Plasmodium knowlesi. The FyFy genotype is found predominantly African and American blacks, who the only groups completely infection P. vivax. To determine if vivax resistance factor, we performed blood typing on 11 black six white volunteers had been exposed bites of vivax-infected mosquitoes. Only five blacks were erythrocytic infection; remaining all whites Duffy-positive determinants (Fya or Fyb...
The gene for the circumsporozoite (CS) protein of Plasmodium falciparum has been cloned and its nucleotide sequence determined. encodes a 412 amino acids as deduced from sequence. contains 41 tandem repeats tetrapeptide, 37 which are Asn-Ala-Asn-Pro four Asn-Val-Asp-Pro. Monoclonal antibodies against CS were inhibited binding to by synthetic peptides repeat simian malaria parasite, knowlesi, have two regions homology, one is present on either side repeat. One region 12 13 identical acids....
Duffy blood group negative human erythrocytes ( FyFy ) are resistant to infection by Plasmodium knowlesi , a simian malaria that infects positive erythrocytes. The P. resistance factor, erythrocytes, occurs in high frequency West Africa, where the people vivax malaria. This suggests determinants Fy or b may be erythrocyte receptors for .
Plasmodium vivax and P. falciparum are the major causes of human malaria, except in sub-Saharan Africa where people lack Duffy blood group antigen, erythrocyte receptor for . negative erythrocytes resistant to invasion by related monkey knowlesi Several lines evidence present study indicate that antigen is chemokines interleukin-8 (IL-8) melanoma growth stimulatory activity (MGSA). First, IL-8 binds minimally erythrocytes. Second, a monoclonal antibody blocked binding other positive Third,...
A 175-kilodalton erythrocyte binding protein, EBA-175, of the parasite Plasmodium falciparum mediates invasion erythrocytes. The receptor for EBA-175 is dependent on sialic acid. domain that binds erythrocytes was identified as region II with use truncated portions expressed COS cells. Region II, which contains a cysteine-rich motif, and native bind specifically to glycophorin A, but not B, membrane. Erythrocyte recognition requires both acid peptide backbone A. identification ligand domains...
The anopheline mosquito is the target in most malaria control programs, primarily through use of residual insecticides. A was studied that refractory to species a genetically controlled mechanism. strain Anopheles gambiae, which selected for complete refractoriness simian parasite Plasmodium cynomolgi, also has varying degrees other examined, including human parasites P. falciparum, ovale, and vivax this principal African vector. Furthermore, extends subhuman primate malarias, rodent...
In recent malaria sporozoite vaccine trials in humans and mice, antibodies to the coat protein have given only modest protection against challenge. contrast, irradiated sporozoites can protect mice massive infections. Evidence suggests that immunity these is mediated by T cells. To identify mechanism of immunity, we used monoclonal specific for either CD4 or CD8 molecule selectively deplete sporozoite-immunized T-cell subsets. Though vivo depletion CD4+ cells did not reduce CD8+ abolished...
Malaria erythrocyte binding proteins use the Duffy blood group antigen (Plasmodium vivax and Plasmodium knowlesi) sialic acid falciparum) on surface as receptors. We had previously cloned one P. gene, falciparum part of three knowlesi genes encoding these described homology between genes. have completed cloning sequencing identified introns in that correct published deduced amino sequences. All similar structures, with or two exons signal sequence domain, an exon transmembrane cytoplasmic...
We have identified strain-specific antigens with Camp and St. Lucia strains of P. falciparum Mr approximately 285,000 260,000, respectively. These were metabolically labeled radioactive amino acids, indicating that they parasite origin rather than altered host components. proteins had the properties a molecule exposed on surface infected erythrocytes (IE). First, are accessible to lactoperoxidase-catalyzed radioiodination IE. Second, radioiodinated cleaved by low concentrations trypsin (0.1...
An electro-optical system was developed to record microscope images with high resolution at low light intensities. The used study the invasion of erythrocytes by malaria merozoites. Invasion consists attachment anterior end parasite erythrocyte, deformation and entry erythrocyte membrane invagination.
Background Pfs25 and Pvs25, surface proteins of mosquito stage the malaria parasites P. falciparum vivax, respectively, are leading candidates for vaccines preventing transmission by mosquitoes. This single blinded, dose escalating, controlled Phase 1 study assessed safety immunogenicity recombinant Pvs25 formulated with Montanide ISA 51, a water-in-oil emulsion. Methodology/Principal Findings The trial was conducted at Johns Hopkins Center Immunization Research, Washington DC, USA, between...
Plasmodium vivax and the related monkey malaria, P. knowlesi, require interaction with Duffy blood group antigen, a receptor for family of chemokines that includes interleukin 8, to invade human erythrocytes. One three knowlesi proteins serve as erythrocyte binding ligands in such interactions share sequence homology. Expression different regions protein COS7 cells identified cysteine-rich domain bound group-positive but not group-negative The homologous also erythrocytes, had specificities....
Abs are central to malaria immunity, which is only acquired after years of exposure Plasmodium falciparum ( Pf ). Despite the enormous worldwide burden malaria, targets protective and basis their inefficient acquisition unknown. Addressing these knowledge gaps could accelerate vaccine development. To this end, we developed a protein microarray containing ∼23% 5,400-protein proteome used array probe plasma from 220 individuals between ages 2–10 18–25 in Mali before 6-month season. Episodes...
We have previously demonstrated that invasion of erythrocytes (RBCs) by malaria merozoites follows a sequence: recognition and attachment in an apical orientation associated with widespread deformation the RBC, junction formation, movement around merozoite brings into invaginated RBC membrane, sealing membrane. In present paper, we describe method for blocking at early stage sequence. Cytochalasin-treated attach specifically to host RBCs, most frequently region contains specialized...
Erythrocytes infected with the late stages of human malarial parasite Plasmodium falciparum became attached to a subpopulation cultured endothelial cells by knoblike protrusions on surface erythrocytes. Infected erythrocytes did not bind fibroblasts; uninfected either or fibroblasts. The results suggest specific receptor-ligand interaction between and component, components, in knobs
The commitment of Plasmodium merozoites to invade red blood cells (RBCs) is marked by the formation a junction between merozoite and RBC coordinated induction parasitophorous vacuole. Despite its importance, molecular events underlying parasite’s invasion are not well understood. Here we show that interaction two parasite proteins, RON2 AMA1, known be critical for invasion, essential trigger formation. Using antibodies (Abs) bind near hydrophobic pocket AMA1 mutated in pocket, identified...