A5101746088- Chromosomal and Genetic Variations
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Polysaccharides and Plant Cell Walls
- Renal and related cancers
- Ginseng Biological Effects and Applications
- Cystic Fibrosis Research Advances
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Reproductive Biology and Fertility
- Medicinal Plant Pharmacodynamics Research
- Sperm and Testicular Function
- Cardiac electrophysiology and arrhythmias
- Intracerebral and Subarachnoid Hemorrhage Research
- Phytoestrogen effects and research
- Immunodeficiency and Autoimmune Disorders
- Peptidase Inhibition and Analysis
- Cardiac Arrhythmias and Treatments
- Genetics and Neurodevelopmental Disorders
- Traditional Chinese Medicine Studies
- Effects and risks of endocrine disrupting chemicals
- Viral Infections and Immunology Research
- Protist diversity and phylogeny
Daping Hospital
2025
Army Medical University
2025
Institute of Zoology
2020-2024
Chinese Academy of Sciences
2020-2024
Southwest Medical University
2024
Guangzhou Medical University
2022-2023
University of Chinese Academy of Sciences
2020-2023
University of California, Los Angeles
2023
Peking University
2023
State Key Laboratory of Stem Cell and Reproductive Biology
2023
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causal pathogen of ongoing global pandemic disease 2019 (COVID-19). Loss smell and taste are symptoms COVID-19, may be related to cilia dysfunction. Here, we found that SARS-CoV-2 ORF10 increases overall E3 ligase activity CUL2ZYG11B complex by interacting with ZYG11B. Enhanced causes increased ubiquitination subsequent proteasome-mediated degradation an intraflagellar transport (IFT) B protein, IFT46, thereby impairing both...
Primary ovarian insufficiency (POI), also known as premature failure, is an defect in humans characterized by the depletion of follicles before age 40. However, mechanisms underlying POI remain largely unknown. Here, we show that knockout Epg5 (ectopic P-granules autophagy protein 5 homolog (C. elegans)) results subfertility female mice, which exhibit a POI-like phenotype. Single-cell RNA sequencing analysis revealed affected differentiation granulosa cells (GCs). Further investigation...
Cardiac fibroblasts (CF) express adenosine (ADO) receptors, and pharmacological evidence suggests the possible involvement of A2 (A2a A2b) receptor (A2aR A2bR) subtypes in inhibiting cell functions involved fibrosis. The main objective this study was to define contributions A2a and/or A2b receptors modulating ADO-induced decreases CF functions. For purpose, were either treated pharmacologically or had A2aR A2bR levels modified through use recombinant adenovirus siRNA. assessment mRNA...
Lipid droplet is a dynamic organelle that undergoes periods of biogenesis and degradation under environmental stimuli. The excessive accumulation lipid droplets the major characteristic non-alcoholic fatty liver disease (NAFLD). Moderate aerobic exercise powerful intervention protecting against progress NAFLD. However, its impact on dynamics remains ambiguous. Mice were fed with 15 weeks high-fat diet in order to induce Meanwhile, mice performed treadmill exercise. Our results showed regular...
The landscape of extrachromosomal circular DNA (eccDNA) during mammalian spermatogenesis, as well the biogenesis mechanism, remains to be explored. Here, we revealed widespread eccDNA formation in human sperms and mouse spermatogenesis. We noted that germline eccDNAs are derived from oligonucleosomal fragmentation cells likely undergoing cell death, providing a potential new way for quality assessment sperms. Interestingly, small-sized associated with euchromatin, while large-sized ones...
Meiotic recombination is initiated by Spo11-dependent programmed DNA double-strand breaks (DSBs) that are preferentially concentrated within genomic regions called hotspots; however, the factor(s) specify positions of meiotic DSB hotspots remain unclear. Here, we examined frequency and distribution R-loops, a type functional chromatin structure comprising single-stranded DNA:RNA hybrid, during budding yeast meiosis found R-loops were changed dramatically throughout meiosis. We detected...
The body of tremella were decocted with water, and hydrolyzed 0.1 mol/L hydrochloric acid for different times, giving polysaccharides six molecular mass values. structures all the had non-reducing terminals β-D-pyranglucuronide, backbone was composed (1→3)-linked β-D-manno-pyranoside, side chain (1→6)-linked β-D-xylopyranoside attached to C2 mannopyranoside. Immunomodulatory effect studies indicated that increased counts leukocytes in peripheral blood which significantly lowered by...
Infertility affects 10%-20% of the population in most countries, with approximately half those cases resulting from male infertility. Although millions infertile men are able to have children assistance reproductive technology, individuals non-obstructive azoospermia (NOA) syndrome unable do so because they lack functional sperm. Therefore, some other strategies for NOA still urgently needed. Our current study uses an NOA-like mouse model optimize microinjection and a subsequent...
Abstract Objectives Cell reprogramming has significant impacts on their potential application in regenerative medicine. Chromatin remodelling plays a very important role cell reprogramming, but its underlying mechanism remains poorly understood. Materials and methods RNA‐seq, quantitative RT‐PCR western blot analysis were applied to study the of RNF20 H2B ubiquitination during mouse somatic reprogramming. structure recruitment transcription factors analysed by ChIP‐seq, micrococcal nuclease...
Abstract The landscape of extrachromosomal circular DNA (eccDNA) during mammalian spermatogenesis, as well the biogenesis mechanism remains to be explored. Here, we revealed widespread eccDNA formation in human sperms and mouse spermatogenesis. We noted that germline eccDNAs are derived from oligonucleosomal fragmentation cells likely undergoing cell death, providing a potential new way for quality assessment sperms. Interestingly, small-sized associated with euchromatin, while large-sized...
Abstract Meiosis increases genetic diversity, yet the genome complement needs to be stable ensure offspring viability. Both small ubiquitin‐like modifier (SUMO) and ubiquitin have been reported participate in meiotic regulation, functions of SUMO‐ubiquitination crosstalk meiosis remain unclear. Here, it is that a SUMO‐targeted ligase, Slx8p, promotes accurate chromosome segregation during meiosis, since deletion SLX8 leads increased aneuploidy due defect synaptonemal complex (SC) component...
Background As a group of membrane-anchored proteins, the proteins containing disintegrin and metalloprotease domain (ADAMs) control many biological processes, especially for male fertility. Mouse Adam21 was previously found to be specifically expressed in somatic cells germ testes, but its functional role during spermatogenesis reproductive processes is still unknown. Methods -null mice were created using CRISPR/Cas9 system. Quantitative real-time PCR used analyzing gene expression....
Background Infertility is recognized as a common and worrisome problem of human reproduction worldwide. Based on previous studies, male factors account for about half all infertility cases. Exposure to environmental toxicants an important contributor infertility. Bisphenol A (BPA) the most prominent toxic contaminant worldwide affecting reproductive system. BPA can impair function Golgi apparatus which in spermatogenesis. GGA1 known Golgi-localized, gamma adaptin ear-containing, ARF-binding...
The landscape of extrachromosomal circular DNA (eccDNA) during mammalian spermatogenesis, as well the biogenesis mechanism remains to be explored. Here, we revealed widespread eccDNA formation in human sperms and mouse spermatogenesis. We noted that germline eccDNAs are derived from oligonucleosomal fragmentation cells likely undergoing cell death. Interestingly, small-sized associated with euchromatin, while large-sized ones preferentially generated heterochromatin. By comparing sperm...