A5080478288- RNA Research and Splicing
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Endometriosis Research and Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Molecular Biology Techniques and Applications
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Reproductive tract infections research
- Click Chemistry and Applications
- Birth, Development, and Health
- RNA Interference and Gene Delivery
- Pregnancy and preeclampsia studies
- Pregnancy-related medical research
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Biotin and Related Studies
- Tissue Engineering and Regenerative Medicine
- Estrogen and related hormone effects
- Renal and related cancers
- Genetics and Neurodevelopmental Disorders
- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
Nanjing Drum Tower Hospital
2020-2024
Nanjing Medical University
2017-2024
Nanjing University
2022-2023
Institut de Médecine de la Reproduction
2022-2023
Hainan University
2023
Xijing University
2022
Transcription factors of the Sox protein family contain a DNA-binding HMG box and are key regulators progenitor cell fate. Here, we report that expression Sox30 is restricted to meiotic spermatocytes postmeiotic haploids. mutant males sterile due spermiogenic arrest at early round spermatid stage. Specifically, in absence Sox30, proacrosomic vesicles fail form single acrosomal organelle, spermatids step 2-3. Although most progress through meiosis, accumulation diplotene indicates delayed or...
Despite continuous expansion of the RNA binding protein (RBP) world, there is a lack systematic understanding RBPs in mammalian testis, which harbors one most complex tissue transcriptomes. We adapted interactome capture to mouse male germ cells, building an RBP atlas characterized by multiple layers dynamics along spermatogenesis. Trapping RNA-cross-linked peptides showed that glutamic acid-arginine (ER) patch, residue-coevolved polyampholytic element present coiled coils, enhances its host...
Spermatogenesis requires a large number of proteins to be properly expressed at certain stages, during which post-transcriptional regulation plays an important role. RNA-binding (RBPs) are key players in regulation, but only few RBPs have been recognized and preliminary explored their function spermatogenesis present. Here we identified new RBP tubby-like protein 2 (TULP2) found three potential deleterious missense mutations Tulp2 gene dyszoospermia patients. Therefore, the mechanism TULP2...
RNA regulation by RNA-binding proteins (RBPs) involve extremely complicated mechanisms. MOV10 and MOV10L1 are two homologous helicases implicated in distinct intracellular pathways. participates specifically Piwi-interacting (piRNA) biogenesis protects mouse male fertility. In contrast, the functional complexity of remains incompletely understood, its role mammalian germline is unknown. Here, we report a study biological molecular functions helicase germ cells. nucleocytoplasmic protein...
Abstract Follicle development is a complex dynamic process. The follicles are encapsulated in the stroma, and once follicle develops, moves from cortex to medulla finally ovulate process of continuous growth. Many these processes cannot be explained with alone. Through single-cell spatial transcriptome sequencing at key time points follicular mice after birth, we found that ovarian stromal cells not only one main cell groups make up ovary but their population location also closely related...
Gonadal white adipose tissue (gWAT) can regulate gametogenesis via modulation of neuroendocrine signaling. However, the effect gWAT on local microenvironment gonad was largely unknown. Herein, we ruled out that had a function through unilateral lipectomy strategy, in which cutting off epididymal could reduce seminiferous tubule thickness and decrease sperm counts only adjacent testis epididymis affected gonad. Consistent with results males, females, ovary mass similarly decreased by...
This study aims to compare the prevalence of sexually transmitted infections (STIs) with semen quality in men from couples primary and secondary infertility. Semen samples were collected 133 who requested fertility evaluation. Seminal tract infection Ureaplasma spp. (UU), Mycoplasma hominis (MH), genitalium (MG), Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), herpes simplex virus-2 (HSV-2) was assessed by PCR-based diagnostic assays. Among all patients, STIs higher infertility than...
Decidualization is a prerequisite for successful embryo implantation, in which elongated fibroblast-like endometrial stromal cells differentiate into more rounded decidual cells. Accumulating evidence has stressed the important role of defective eutopic endometrium infertility endometriosis patients. However, arginine methylation process physiological decidualization and pathological defects not clear. Here, we observed that expression level PRMT5, main type II PRMT, was decreased patients,...
The role of sexually transmitted infections (STIs) in semen parameters and male infertility is still a controversial area. Previous studies have found bacterial infection minority infertile leukocytospermic males. This study aims to investigate the prevalence STIs from subfertile men with leukocytospermia (LCS) without (non-LCS) their associations sperm quality.Semen samples were collected 195 who asked for fertility evaluation. Infection above 6 pathogens was assessed each sample. Sperm...
Abstract Liver serine/threonine kinase B1 (LKB1) is a tumor suppressor associated with the pathogenesis of Peutz-Jeghers syndrome. Affected males are at increased risk developing Sertoli cell tumors and display defective spermatogenesis. Male mice lacking short isoform ( Lkb1 S ) were sterile exhibited abnormal spermiogenesis. In addition to isoform, long L also expressed in testis; however, requirement for fertility functional difference between isoforms remain unknown. Herein, different...
Spermatogenesis defines a highly ordered process of male germ cell differentiation in mammals. In testis, transcription and translation are uncoupled, underlining the importance post-transcriptional regulation gene expression orchestrated by RBPs. To elucidate mechanistic roles an RBP, crosslinking immunoprecipitation (CLIP) methodology can be used to capture its endogenous direct RNA targets define actual interaction sites. The enhanced CLIP (eCLIP) is newly-developed method that offers...
The DNAJ family of molecular chaperones maintains protein homeostasis in mitotic and postmeiotic cells, especially germ cells. Recently, we found that the transcription factor SOX30 initiates Dnajb8 during late meiosis spermiogenesis mouse testes.We used CRISPR/Cas9 system to generate mutant mice analyze phenotype mutants.Although is an evolutionarily conserved gene, it not essential for spermatogenesis male fertility. We provide this phenotypic information, which could prevent duplicative...
Recurrent implantation failure (RIF) is a rather thorny problem in the clinical practice of assisted reproductive technology. Due to complex aetiology RIF, its pathogenesis far from fully understood, and there no effective treatment available. Here, We explored regulatory mechanism four half-domains LIM domain 1 (FHL1), which significantly downregulated endometrium RIF patients, blastocyst-epithelial adhesion. Indeed, FHL1 expression was dramatically increased normal female mid-secretory...
Abstract Background DNAJBs are highly conserved proteins that involved in various biological processes. Although several expressed the testis, function of DNAJB7 spermatogenesis and male fertility remains unclear. Methods To identify role reproduction process, Dnajb7- deficient mice were generated by CRISPR/Cas9-mediated genome editing system. Histological immunofluorescence assays performed to analyze phenotype Dnajb7 mutants. Results is specifically haploid germ cells. knockout fertile do...
Mechanistic target of rapamycin (mTOR) controls cell growth and metabolism in response to environmental metabolic signals. Rapamycin robustly extends the lifespan mammals has clinical relevance organ transplantation cancer therapy but side effects include male infertility. Here, we report that chronic treatment causes spermatogenic arrest adult mice due defects sex body formation meiotic chromosome inactivation (MSCI). Many chromosome-linked genes were up-regulated isolated pachytene...
Protein-nucleic acid interactions play important roles in biological processes such as transcription, recombination, and RNA metabolism. Experimental methods to study protein-nucleic require the use of fluorescent tags, radioactive isotopes, or other labels detect analyze specific target molecules. Biotin, a non-radioactive nucleic label, is commonly used electrophoretic mobility shift assays (EMSA) but has not been regularly employed monitor protein activity during processes. This protocol...
Abstract Although hundreds of knockout mice show infertility as a major phenotype, the causative genic mutations male in humans remain rather limited. Here, we report identification missense mutation (D136G) X-linked TAF7L gene potential cause oligozoospermia men. The human aspartate (D136) is evolutionally conserved across species, and its change to glycine (G) predicted be detrimental. Genetic complementation experiments budding yeast demonstrate that or analogous asparagine (N) residue...
Protein-nucleic acid interactions play important roles in biological processes such as transcription, recombination, and RNA metabolism. Experimental methods to study protein-nucleic require the use of fluorescent tags, radioactive isotopes, or other labels detect analyze specific target molecules. Biotin, a non-radioactive nucleic label, is commonly used electrophoretic mobility shift assays (EMSA) but has not been regularly employed monitor protein activity during processes. This protocol...
Raised-floor data centers usually suffer from the local hotspots resulted uneven cool air delivery. These not only degrade server performance, but also threat equipment reliability. The commonly used industrial practice of increasing Computer Room Air Conditioner (CRAC) blower speed for removing is energy inefficient and may lead to overcooling some servers. In this paper, we explore potential active tiles in center cooling management. particular, deploy a prototype tile production conduct...
Spermatogenesis defines a highly ordered process of male germ cell differentiation in mammals. In testis, transcription and translation are uncoupled, underlining the importance post-transcriptional regulation gene expression orchestrated by RBPs. To elucidate mechanistic roles an RBP, crosslinking immunoprecipitation (CLIP) methodology can be used to capture its endogenous direct RNA targets define actual interaction sites. The enhanced CLIP (eCLIP) is newly-developed method that offers...
In the task of assisted coherence distillation via set operations X, where X is either local incoherent and classical communication (LICC), quantum-incoherent (LQICC), separable (SI), or quantum (SQI), two parties, namely Alice Bob, share many copies a bipartite joint state. The aim process to generate maximal possible on subsystem Bob. this paper, we investigate some special mixed states, states with vanished basis-dependent discord Werner states. We show that all four sets are equivalent...