A5103076735- Cellular transport and secretion
- Sperm and Testicular Function
- Cellular Mechanics and Interactions
- Neurofibromatosis and Schwannoma Cases
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Reproductive Biology and Fertility
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hippo pathway signaling and YAP/TAZ
- Urological Disorders and Treatments
- Signaling Pathways in Disease
- Cancer-related gene regulation
- Peroxisome Proliferator-Activated Receptors
- Glycosylation and Glycoproteins Research
- Prenatal Screening and Diagnostics
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Lipid Membrane Structure and Behavior
- Cell Adhesion Molecules Research
- Optical measurement and interference techniques
- Phagocytosis and Immune Regulation
Anhui Medical University
2015-2024
People’s Hospital of Rizhao
2017-2024
Hefei National Center for Physical Sciences at Nanoscale
2006-2019
University of Science and Technology of China
2005-2017
Morehouse School of Medicine
2006-2017
Center for Excellence in Molecular Cell Science
2017
Xiamen University
2006
University of Tehran
2006
Northwest A&F University
2006
Imperial College London
2006
Chromosome segregation in mitosis is orchestrated by dynamic interaction between spindle microtubules and the kinetochore. Septin (SEPT) belongs to a conserved family of polymerizing GTPases localized metaphase during mitosis. Previous study showed that SEPT2 depletion results chromosome mis-segregation correlated with loss centromere-associated protein E (CENP-E) from kinetochores congressing chromosomes (1). However, it has remained elusive as whether CENP-E physically interacts SEPT how...
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in world and metastasis leading cause death associated with HCC. Hypoxia triggers epithelial-mesenchymal transition (EMT) cancer cells, which enhances their character elevates metastatic risk. Supervillin associates tightly membrane cytoskeleton, promoting cell motility, invasiveness, survival. However, roles supervillin HCC remain unclear.Tissue microarray technology was used to immunohistochemically stain for...
Endoplasmic reticulum (ER) stress is involved in ischemic preconditioning that protects various organs from ischemia/reperfusion (I/R) injury. We established an vivo ER model which tunicamycin was injected into rats before hepatic I/R. The I/R injury, demonstrated by serum aminotransferase level and the ultra-structure of liver, alleviated administration tunicamycin, induced rat liver activating inositol-requiring enzyme 1 (IRE1) upregulating 78 kDa glucose-regulated protein (GRP78)....
Ribosomes mediate protein synthesis, which is one of the most energy-demanding activities within cell, and mitochondria are main sources generating energy. How mitochondrial morphology functions adjusted to cope with ribosomal defects, can impair synthesis affect cell viability, poorly understood. Here, we used fission yeast Schizosaccharomyces Pombe as a model organism investigate interplay between ribosome mitochondria. We found that insult, caused by absence Rpl2702, activates signaling...
The ezrin-radixin-moesin proteins provide a regulated linkage between membrane and the cortical cytoskeleton also participate in signal transduction pathways. Ezrin is localized to apical of parietal cells couples protein kinase A activation cascade HCl secretion. Our recent proteomic study revealed complex ezrin-ACAP4-ARF6 essential for volatile remodeling (Fang, Z., Miao, Y., Ding, X., Deng, H., Liu, S., Wang, F., Zhou, R., Watson, C., Fu, Hu, Q., Lillard, J. W., Jr., Powell, M., Chen,...
Glycosphingolipid (GSL) metabolism is involved in various physiological processes, including all major cell signaling pathways, and its dysregulation linked to some diseases. The four-phosphate adaptor protein FAPP2-mediated glucosylceramide (GlcCer) transport for complex GSL synthesis has been studied extensively. However, the molecular machinery of FAPP2 as a GlcCer-transferring remains poorly defined. Here, we identify Golgi-resident protein, acyl-coenzyme A binding domain containing 3...
Quantitative differential phase contrast (qDPC) microscopy can achieve imaging of unlabeled cell samples with high spatial resolution. However, qDPC is easily affected by the noise generated in experiments. In this manuscript, L0-norm regularization was introduced into reconstruction for sparse (L0-qDPC), including quantitative target and samples. After detailed comparison fidelity, contrast, resolution, convergence rate, L0-qDPC method provide a stable without parameter adjustment due to...
ARF6 GTPase is a conserved regulator of membrane trafficking and actin-based cytoskeleton dynamics at the leading edge migrating cells. A key determinant function lifetime GTP-bound active state, which orchestrated by GTPase-activating protein (GAP) GTP-GDP exchanging factor. However, very little known about molecular mechanisms underlying ARF6-mediated cell migration. To systematically analyze proteins that regulate activity during migration, we performed proteomic analysis selectively...
The ERM (ezrin/radixin/moesin) proteins provide a regulated linkage between membrane and the cortical cytoskeleton also participate in signal transduction pathways. Ezrin is localized to apical of parietal cells couples protein kinase A activation cascade HCl secretion gastric cells. Here, we show that integrity ezrin essential for cell first evidence interacts with PALS1, an evolutionarily conserved PDZ SH3 domain-containing protein. Our biochemical study verifies binds PALS1 via its N...
Abstract Progression of hepatocellular carcinoma involves multiple genetic and epigenetic alterations that promote cancer invasion metastasis. Our recent study revealed hyperphosphorylation ezrin promotes intrahepatic metastasis in vivo cell migration vitro. Celastrol is a natural product from traditional Chinese medicine which has been used treating liver cancer. However, the mechanism action underlying celastrol treatment was less clear. Here we show ROCK2 novel target inhibition...
Identification of hub genes and key pathways gastric cancer was recognized to be essential elucidate the tumorigenesis GC.This study aimed identify differentially expressed (DEGs) in GC via bioinformatics methods their related involved pathological process GC.Gene expression profile datasets acquired by microarray chips or RNA-seq were downloaded from GEO dataset TCGA, 298 identified.The Gene Ontology (GO) Kyoto Genomic Encyclopedia (KEGG) DEGs then analyzed DAVID database potential...
Human oocyte maturation arrest represents one of the severe conditions for female patients with primary infertility. However, genetic factors underlying this human disease remain largely unknown. The spindle assembly checkpoint (SAC) is an intricate surveillance mechanism that ensures accurate segregation chromosomes throughout cell cycles. Once kinetochores are correctly attached to bipolar spindles and SAC satisfied, MAD2L1BP, best known as p31comet, binds mitosis deficient 2 (MAD2)...
The cold shock domain (CSD) is an evolutionarily conserved nucleic acid binding that exhibits activity to RNA, ssDNA, and dsDNA. Mammalian CRHSP-24 contains CSD, but its structure-functional relationship has remained elusive. Here we report the crystal structure of human characterization molecular trafficking between stress granules processing bodies in response oxidative stress. determined by single-wavelength anomalous dispersion α-helix a compact β-barrel formed five curved anti-parallel...
Abstract The cytoskeleton plays a critical role in regulating mitochondria distribution. Similar to axonal mitochondria, the fission yeast are distributed by microtubule cytoskeleton, but this is regulated motor-independent mechanism depending on associated protein mmb1p as absence of causes aggregation. In study, using series chimeric proteins control subcellular localization and motility we show that molecule containing binding domain outer membrane tom22p can restore normal interconnected...
Abstract Ezrin, a membrane–cytoskeleton linker protein, plays an essential role in cell polarity establishment, migration, and division. Recent studies show that ezrin phosphorylation regulates breast cancer metastasis by promoting survivor promotes intrahepatic via migration. However, it was less characterized whether there are additional post-translational modifications and/or crosstalks on underlying context-dependent migration invasion. Here we is acetylated p300/CBP-associated factor...
Ezrin–radixin–moesin protein family provides a regulated link between the cortical actin cytoskeleton and plasma membrane. Phosphorylation of ezrin has been functionally linked to membrane dynamics plasticity. Our recent study demonstrated that phosphorylation conserved T567 residue alters physiology gastric parietal cells. However, molecular mechanism phosphorylation‐induced activation remained elusive. Here we use atomic force microscopy (AFM) probe phosphorylation‐mediated in single...
ArfGAP With Coiled-Coil, Ankyrin Repeat And PH Domains 4 (ACAP4) is an ADP-ribosylation factor 6 (ARF6) GTPase-activating protein essential for EGF-elicited cell migration. However, how ACAP4 regulates membrane dynamics and curvature in response to EGF stimulation unknown. Here, we show that phosphorylation of the N-terminal region ACAP4, named Bin, Amphiphysin, RSV161/167 (BAR) domain, at Tyr34 necessary remodeling. Domain structure analysis demonstrates BAR domain curvature. cells causes...
ARF6 GTPase is an important regulator of membrane trafficking and actin-based cytoskeleton dynamics active at the leading edge migrating cells. The integrin family heterodimeric transmembrane proteins serve as major receptors for extracellular matrix proteins, which play essential roles in cell adhesion migration. Our recent proteomic analyses effectors have identified a novel GTPase-activating protein, ACAP4, EGF-induced However, molecular mechanisms underlying ACAP4-mediated migration...
Digestion in the stomach depends on acidification of lumen. Histamine-elicited acid secretion is triggered by activation PKA cascade, which ultimately results insertion gastric H,K-ATPases into apical plasma membranes parietal cells. Our recent study revealed functional role PKA–MST4–ezrin signaling axis histamine-elicited secretion. However, it remains uncharacterized how operates Here we show that MST4 phosphorylates ACAP4, an ARF6 GTPase-activating protein, at Thr545. Histamine...
Although extensive development has been made in the treatment of inflammatory bowel disease (IBD), adverse effects and incomplete efficacy currently used medications are continuous challenge. Accumulated reports on benefits chitosan oligosaccharides intestinal disorders make chitotriose (COS) a breakthrough new IBD drugs. This study aimed to investigate biosafety, pharmacological mechanisms COS experimental compare with commercial 5-Aminosalicylic acid (5-ASA). In this study, effectively...