A5056028884- Enzyme Structure and Function
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Animal Virus Infections Studies
- Computational Drug Discovery Methods
- Tuberculosis Research and Epidemiology
- Protein Structure and Dynamics
- ATP Synthase and ATPases Research
- Pharmacogenetics and Drug Metabolism
- Bacteriophages and microbial interactions
- interferon and immune responses
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Signaling Pathways in Disease
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Drug Transport and Resistance Mechanisms
- Heat shock proteins research
- Mitochondrial Function and Pathology
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Viral Infections and Vectors
Tsinghua University
2016-2025
ShanghaiTech University
2017-2025
Nankai University
2016-2025
Shenzhen Third People’s Hospital
2023-2025
Guangzhou Experimental Station
2022-2025
Institute of Biophysics
2015-2024
Chinese Academy of Sciences
2015-2024
State Key Laboratory of Medicinal Chemical Biology
2012-2024
Fudan University
2024
Tianjin University
2020-2024
The COVID-19 RNA-synthesizing machine Many in the scientific community have mobilized to understand virus that is causing global coronavirus disease 2019 (COVID-19) pandemic. Gao et al. focused on a complex plays key role replication and transcription cycle of virus. They used cryo–electron microscopy determine 2.9-angstrom-resolution structure RNA-dependent RNA polymerase nsp12, which catalyzes synthesis viral RNA, with two cofactors, nsp7 nsp8. nsp12 target for nucleotide analog antiviral...
SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is the etiological agent responsible for global COVID-19 (coronavirus disease 2019) outbreak. The main protease of SARS-CoV-2, Mpro, a key enzyme that plays pivotal role in mediating viral replication and transcription. We designed synthesized two lead compounds (11a 11b) targeting Mpro Both exhibited excellent inhibitory activity potent anti-SARS-CoV-2 infection activity. x-ray crystal structures complex with 11a or 11b, both...
A newly identified severe acute respiratory syndrome coronavirus (SARS-CoV), is the etiological agent responsible for outbreak of SARS. The SARS-CoV main protease, which a 33.8-kDa protease (also called 3C-like protease), plays pivotal role in mediating viral replication and transcription functions through extensive proteolytic processing two replicase polyproteins, pp1a (486 kDa) pp1ab (790 kDa). Here, we report crystal structures at different pH values complex with specific inhibitor....
The genus Coronavirus contains about 25 species of coronaviruses (CoVs), which are important pathogens causing highly prevalent diseases and often severe or fatal in humans animals. No licensed specific drugs available to prevent their infection. Different host receptors for cellular entry, poorly conserved structural proteins (antigens), the high mutation recombination rates CoVs pose a significant problem development wide-spectrum anti-CoV vaccines. CoV main proteases (M(pro)s), key...
Significance Proofreading exonucleases contributing to replication fidelity in DNA viruses and cellular organisms are well known; however, proofreading RNA was unknown until recently. Coronavirus nonstructural protein 14 (nsp14) has been shown function as a exoribonuclease. Additionally, nsp14 shows (guanine-N7) methyl transferase activity for viral mRNA capping. Both roles important transcription. Here, we report the structures of severe acute respiratory syndrome-coronavirus complex with...
ABSTRACT Coronaviruses (CoVs) can infect humans and multiple species of animals, causing a wide spectrum diseases. The coronavirus main protease (M pro ), which plays pivotal role in viral gene expression replication through the proteolytic processing replicase polyproteins, is an attractive target for anti-CoV drug design. In this study, crystal structures infectious bronchitis virus (IBV) M severe acute respiratory syndrome CoV (SARS-CoV) mutant (H41A), complex with N-terminal autocleavage...
A steric block to SARS-CoV-2 In response infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the immune system makes antibodies, many of which target spike protein, a key player in host cell entry. Antibodies that potently neutralize virus hold promise as therapeutics and could inform vaccine design. Lv et al. report humanized monoclonal antibody protected against mouse model. The cryo–electron microscopy structure, together with biochemical, cellular, virological...
Unveiling African swine fever virus (ASFV) is highly contagious and often lethal. With no vaccine or effective treatment, infections require large-scale culling of pigs. Wang et al. apply cutting-edge cryo–electron microscopy techniques to determine the structure this very large DNA virus. An 8.8-angstrom-resolution reconstruction shows five layers virus, fourth capsid layer could be reconstructed at 4.8-angstrom resolution. The reveals epitopes in major protein that distinguish ASFV from...
Noroviruses are one of the major causes nonbacterial gastroenteritis epidemics in humans. Recent studies on norovirus receptors show that different noroviruses recognize human histo-blood group antigens (HBGAs), and eight receptor binding patterns have been identified. The P domain capsids is directly involved this recognition. To determine precise locations modes HBGA carbohydrates viral capsids, a recombinant protein GII-4 strain norovirus, VA387, was cocrystallized with synthetic type A...
Abstract To date, an effective therapeutic treatment that confers strong attenuation toward coronaviruses (CoVs) remains elusive. Of all the potential drug targets, helicase of CoVs is considered to be one most important. Here, we first present structure full-length Nsp13 SARS-CoV (SARS-Nsp13) and investigate structural coordination its five domains how these contribute translocation unwinding activity. A model proposed for Upf1-like members according three different conditions in solution...
<i>In vivo</i> induction of non-neuronal cells into neurons by transcription factors offers potential therapeutic approaches for neural regeneration. Although generation induced neuronal (iN) <i>in vitro</i> and has been reported, whether iN can be fully integrated existing circuits remains unclear. Here we show that expression achaete-scute complex homolog-like 1 (<i>Ascl1</i>) alone is sufficient to convert dorsal midbrain astrocytes mice functional vivo</i>. Specific <i>Ascl1</i> in...