A5100347575- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Virology and Viral Diseases
- SARS-CoV-2 and COVID-19 Research
- Malaria Research and Control
- COVID-19 Clinical Research Studies
- Insect symbiosis and bacterial influences
- Influenza Virus Research Studies
- Vector-borne infectious diseases
- Animal Virus Infections Studies
- Respiratory viral infections research
- Virus-based gene therapy research
- HIV Research and Treatment
- Poxvirus research and outbreaks
- interferon and immune responses
- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Herpesvirus Infections and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA and protein synthesis mechanisms
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- Plant Virus Research Studies
- Animal Disease Management and Epidemiology
Institute of Microbiology
2015-2024
Academy of Military Medical Sciences
2017-2024
Hunan University of Finance and Economics
2023
Chengdu University of Traditional Chinese Medicine
2023
Wuhan Institute of Virology
2013-2022
Czech Academy of Sciences, Institute of Microbiology
2022
Institute of Microbiology
2014-2022
Universidade de São Paulo
2020
Twelfth Guangzhou City People's Hospital
2018-2019
Guangzhou Medical University
2018-2019
Mutation for microcephaly Zika virus infections in humans have been known since 1947. Microcephaly and neuropathologies associated with only reported recently, most prevalently the Americas. Yuan et al. investigated recent stable mutations genome engineered them into a low-virulence ancestral strain (see Perspective by Screaton Mongkolsapaya). A single amino acid substitution (serine to asparagine, S139N) viral precursor membrane protein exacerbated symptoms pregnant mice. The reverse...
A steric block to SARS-CoV-2 In response infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the immune system makes antibodies, many of which target spike protein, a key player in host cell entry. Antibodies that potently neutralize virus hold promise as therapeutics and could inform vaccine design. Lv et al. report humanized monoclonal antibody protected against mouse model. The cryo–electron microscopy structure, together with biochemical, cellular, virological...
The recent Zika virus (ZIKV) epidemic in Latin America coincided with a marked increase microcephaly newborns. However, the causal link between maternal ZIKV infection and malformation of fetal brain has not been firmly established. Here we show vertical transmission mice effect on development. We found that intraperitoneal (i.p.) injection contemporary strain pregnant led to radial glia cells (RGs) dorsal ventricular zone fetuses, primary neural progenitors responsible for cortex...
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a global health emergency because of its rapid spread and high mortality. molecular mechanism interaction between host viral genomic RNA is yet unclear. We demonstrate herein that SARS-CoV-2 RNA, as well the negative-sense dynamically N6-methyladenosine (m6A)-modified human monkey cells. Combined RIP-seq miCLIP analyses total 8 m6A sites at single-base resolution genome. Especially, epidemic...
The re-emerging mpox (formerly monkeypox) virus (MPXV), a member of Orthopoxvirus genus together with variola (VARV) and vaccinia (VACV), has led to public health emergency international concern since July 2022. Inspired by the unprecedent success coronavirus disease 2019 (COVID-19) mRNA vaccines, development safe effective vaccine against MPXV is high priority. Based on our established lipid nanoparticle (LNP)-encapsulated platform, we rationally constructed prepared panel multicomponent...
Although several different flaviviruses may cause encephalitis, Japanese encephalitis virus is the most significant, being responsible for thousands of deaths each year in Asia. The structural and molecular basis this not fully understood. Here, we report cryo-electron microscopy structure mature at near-atomic resolution, which reveals an unusual "hole" on surface, surrounded by five encephalitic-specific motifs implicated receptor binding. Glu138 E, highly conserved encephalitic...
Animal models are critical to understand disease and develop countermeasures for the ongoing epidemics of Zika virus (ZIKV). Here we report a non-human primate model using 2016 contemporary clinical isolate ZIKV. Upon subcutaneous inoculation, rhesus macaques developed fever viremia, with robust excretion ZIKV RNA in urine, saliva, lacrimal fluid. Necropsy two infected animals revealed that systematic infections involving central nervous system visceral organs were established at acute...
Abstract There is an urgent need for animal models to study SARS-CoV-2 pathogenicity. Here, we generate and characterize a novel mouse-adapted strain, MASCp36, that causes severe respiratory symptoms, mortality. Our model exhibits age- gender-related mortality akin COVID-19. Deep sequencing identified three amino acid substitutions, N501Y, Q493H, K417N, at the receptor binding domain (RBD) of during in vivo passaging. All RBD mutations significantly enhance affinity its endogenous receptor,...
Glioblastoma (GBM) is the deadliest type of brain tumor, and glioma stem cells (GSCs) contribute to tumor recurrence therapeutic resistance. Thus, an oncolytic virus targeting GSCs may be useful for improving GBM treatment. Because Zika (ZIKV) has tropism infecting GSCs, we investigated safety efficacy a live attenuated ZIKV vaccine candidate (ZIKV-LAV) treatment human in GSC-derived orthotopic model. Intracerebral injection ZIKV-LAV into mice caused no neurological symptoms or behavioral...
Abstract The global spread of Zika virus (ZIKV) and its unexpected association with congenital defects necessitates the rapid development a safe effective vaccine. Here we report characterization recombinant chimeric ZIKV vaccine candidate (termed ChinZIKV) that expresses prM-E proteins using licensed Japanese encephalitis live-attenuated SA14-14-2 as genetic backbone. ChinZIKV retains replication activity stability in vitro, while exhibiting an attenuation phenotype multiple animal models....
Abstract SARS-CoV-2 infection causes a wide spectrum of clinical manifestations in humans, and olfactory dysfunction is one the most predictive common symptoms COVID-19 patients. However, underlying mechanism by which leads to disorders remains elusive. Herein, we demonstrate that intranasal inoculation with induces robust viral replication epithelium (OE), not bulb (OB), resulting transient humanized ACE2 (hACE2) mice. The sustentacular cells Bowman’s gland OE were identified as major...
The global COVID-19 epidemic has spread rapidly around the world and caused death of more than 5 million people. It is urgent to develop effective strategies treat patients. Here, we revealed that SARS-CoV-2 infection resulted in dysregulation genes associated with NAD+ metabolism, immune response, cell mice, similar We therefore investigated effect treatment its intermediate (NMN) found pneumonia phenotypes, including excessive inflammatory infiltration, hemolysis, embolization...
Viruses that replicate in the cytoplasm cannot access host nuclear capping machinery. These viruses have evolved viral methyltransferase(s) to methylate N-7 and 2'-O cap of their RNA; alternatively, they "snatch" mRNA form 5' end RNA. The function methylation RNA is mimic cellular evade innate immune restriction. A cytoplasmic virus defective replicative, but its lacks recognized eliminated by response. Such a mutant could be rationally designed as live attenuated vaccine. Here, we use...
Japanese encephalitis (JE) remains the leading cause of viral in Asia-Pacific region, and live vaccine SA14-14-2 is currently recommended by WHO widely used Asian countries with a good safety efficacy profile. In this study, we demonstrated that failed to produce NS1’, larger NS1-related protein, compared its parental strain SA14 various cells. Sequence analysis secondary structure prediction identified single silent mutation G66A NS2A-coding region destabilized conserved pseudoknot...
Viral replicase recruitment and long-range RNA interactions are essential for virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue (DENV) Zika (ZIKV). Here, we revealed a highly conserved, conformation-tunable cis-acting element named 5'-UAR-flanking stem (UFS) in flavivirus genomic 5' terminus. We demonstrated that UFS was critical efficient NS5 viral synthesis different flaviviruses. Interestingly,...
cis-Acting elements in the viral genome RNA (vRNA) are essential for translation, replication, and/or encapsidation of viruses. In this study, a novel conserved cis-acting element was identified capsid-coding region mosquito-borne flavivirus. The downstream 5' cyclization sequence (5'CS) pseudoknot (DCS-PK) has three-stem structure, as demonstrated by structure prediction and biochemical analysis. Using dengue virus model, we show that DCS-PK enhances vRNA replication its function depends on...
Mutations and transient conformational movements of the receptor binding domain (RBD) that make neutralizing epitopes momentarily unavailable present immune escape routes for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To mitigate viral escape, we developed a cocktail antibodies (NAbs) targeting located on different domains spike (S) protein. Screening library monoclonal generated from peripheral blood mononuclear cells COVID-19 convalescent patients yielded potent NAbs,...
The high infection rate and rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) make it a world-wide pandemic. Individuals infected by the virus exhibited different degrees symptoms, most convalescent individuals have been shown to develop both cellular humoral immune responses. However, virus-specific adaptive responses in patients during phase not thoroughly studied. Here, we found that group COVID-19 with distress (ARDS) hospitalization, them mounted...
Glioblastoma (GBM) is the deadliest primary brain tumor and generally resistant to immunotherapy because of severe dysfunction T cells. Novel treatment options are critically needed overcome resistance GBM. Here we demonstrate that Zika virus (ZIKV) improves efficacy anti-PD ligand 1 (PD-L1) in We found ZIKV induces a strong pro-inflammatory response increases CD4+ CD8+ cell intratumoral infiltration activation GBM mouse models. mice bearing tumors inhibits growth prolongs survival. These...