A5101708517- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Influenza Virus Research Studies
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Respiratory viral infections research
- HIV, Drug Use, Sexual Risk
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- Immune Response and Inflammation
- Long-Term Effects of COVID-19
- RNA Research and Splicing
- COVID-19 Impact on Reproduction
- vaccines and immunoinformatics approaches
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- COVID-19 diagnosis using AI
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Gynecological conditions and treatments
- Liver Disease Diagnosis and Treatment
Southwest University
2025
Tianjin Centers for Disease Control and Prevention
2024
City University of New York
2024
Renji Hospital
2024
Shanghai Jiao Tong University
2024
Harbin Medical University
2021-2024
Shanxi Medical University
2024
Third Affiliated Hospital of Harbin Medical University
2021-2024
Institute of Microbiology
2014-2023
Wuhan Center for Disease Control and Prevention
2014-2023
Modeling SARS-CoV-2 in mice Among the research tools necessary to develop medical interventions treat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, high on list are informative animal models with which study viral pathogenesis. Gu et al. developed a mouse model strain was infectious and could cause an inflammatory response moderate pneumonia. Adaptation of this appeared be dependent critical amino acid change, Asn 501 Tyr (N501Y), within receptor-binding domain...
BackgroundWuhan was the epicentre of COVID-19 outbreak in China. We aimed to determine seroprevalence and kinetics anti-SARS-CoV-2 antibodies at population level Wuhan inform development vaccination strategies.MethodsIn this longitudinal cross-sectional study, we used a multistage, population-stratified, cluster random sampling method systematically select 100 communities from 13 districts Wuhan. Households were selected each community all family members invited health-care centres...
The pathogenesis of highly pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV) remains poorly understood. In a previous study, we established an hDPP4-transgenic (hDPP4-Tg) mouse model in which MERS-CoV infection causes severe acute failure and high mortality accompanied by elevated secretion cytokines chemokines. Since excessive complement activation is important factor that contributes to lung injury after viral infection, this investigated the role MERS-CoV-induced damage....
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein mediates infection of cells expressing angiotensin-converting enzyme (ACE2). ACE2 is also the viral receptor SARS-CoV (SARS-CoV-1), a related that emerged in 2002–2003. Horseshoe bats (genus Rhinolophus ) are presumed to be original reservoir both viruses, and SARS-like coronavirus, RaTG13, closely SARS-CoV-2, has been identified one horseshoe-bat species. Here we characterize ability S-protein...
SUMMARY The SARS-coronavirus 2 (SARS-CoV-2) spike (S) protein mediates entry of SARS-CoV-2 into cells expressing the angiotensin-converting enzyme (ACE2). S engages ACE2 through its receptor-binding domain (RBD), an independently folded 197-amino acid fragment 1273-amino S-protein protomer. Antibodies to RBD SARS-CoV (SARS-CoV-1), a closely related coronavirus which emerged in 2002-2003, have been shown potently neutralize SARS-CoV-1 S-protein-mediated entry, and presence anti-RBD antibodies...
The acute lung injury (ALI) that occurs after the highly pathogenic avian influenza H5N1 virus infection is associated with an abnormal host innate immune response. Because complement system plays a central role in immunity and because aberrant activation variety of autoimmune inflammatory diseases, we investigated involvement pathogenesis ALI induced by infection. We showed H5N1-infected mice was caused excessive activation, as demonstrated deposition C3, C5b-9, mannose-binding lectin...
Abstract There is an urgent need for animal models to study SARS-CoV-2 pathogenicity. Here, we generate and characterize a novel mouse-adapted strain, MASCp36, that causes severe respiratory symptoms, mortality. Our model exhibits age- gender-related mortality akin COVID-19. Deep sequencing identified three amino acid substitutions, N501Y, Q493H, K417N, at the receptor binding domain (RBD) of during in vivo passaging. All RBD mutations significantly enhance affinity its endogenous receptor,...
Therapeutic development is critical for preventing and treating continual MERS-CoV infections in humans camels. Because of their small size, nanobodies (Nbs) have advantages as antiviral therapeutics (e.g., high expression yield robustness storage transportation) also potential limitations low antigen-binding affinity fast renal clearance). Here, we developed novel Nbs that specifically target the receptor-binding domain (RBD) spike protein. They bind to a conserved site on RBD with...
Mosquito-borne flaviviruses infect both mammals and mosquitoes. RNA interference (RNAi) has been demonstrated as an anti-flavivirus mechanism in mosquitoes; however, whether how induce antagonize RNAi-mediated antiviral immunity remains unknown. We show that the nonstructural protein NS2A of dengue virus-2 (DENV2) act a viral suppressor RNAi (VSR). When NS2A-mediated suppression was disabled, resulting mutant DENV2 induced Dicer-dependent production abundant DENV2-derived siRNAs...
Triosephosphate isomerase 1 (TPI1), as a key glycolytic enzyme, is upregulated in multiple cancers. However, expression profile and regulatory mechanism of TPI1 breast cancer (BRCA) remain mysterious.Western blotting immunohistochemistry (IHC) assays were used to investigate the BRCA specimens cell lines. correlation with clinicopathological characteristics prognosis 362 patients was analyzed using tissue microarray. Overexpression knockdown function experiments cells mice models performed...
The Middle East Respiratory Syndrome Coronavirus (MERS-CoV) causes severe acute respiratory failure and considerable extrapumonary organ dysfuction with substantial high mortality. For the limited number of autopsy reports, small animal models are urgently needed to study mechanisms MERS-CoV infection pathogenesis disease evaluate efficacy therapeutics against infection. In this study, we developed a transgenic mouse model globally expressing codon-optimized human dipeptidyl peptidase 4...
Middle East respiratory syndrome (MERS) is an emerging infectious disease caused by MERS coronavirus (MERS-CoV). The continuous increase of cases has posed a serious threat to public health worldwide, calling for development safe and effective vaccines. We have previously shown that recombinant protein containing residues 377–588 MERS-CoV receptor-binding domain (RBD) fused with human Fc (S377-588-Fc) induced highly potent anti-MERS-CoV neutralizing antibodies in the presence MF59 adjuvant....
This study demonstrated that aberrant complement activation plays an important role in pathogenesis of acute lung injury induced by influenza A(H7N9) virus infection and anti-C5a antibody treatment might be effective new strategy for viral pneumonia. Background. Patients infected with present (ALI) is due to severe pneumonia systemic inflammation. It often fatal because there are few options. Complement has been implicated the virus-induced injury; therefore, we investigated effect targeted...
Middle East respiratory syndrome coronavirus (MERS-CoV) is a highly pathogenic virus with crude mortality rate of ~35%. Previously, we established human DPP4 transgenic (hDPP4-Tg) mouse model in which studied complement overactivation-induced immunopathogenesis. Here, to better understand the pathogenesis MERS-CoV, role pyroptosis THP-1 cells and hDPP4 Tg mice MERS-CoV infection. We found that infection induced over-activation macrophages. The hDPP4-Tg infected overexpressed caspase-1 spleen...
The CRISPR-Cas9 system has been applied to DNA editing with precision in eukaryotic and prokaryotic systems, but it is unable edit RNA directly. A recently developed CRISPR-Cas13a shown be capable of effectively knocking down expression mammalian plant cells. In this study, we employ the achieve reprogrammable inactivation dengue virus Quantitative reverse transcription PCR (qRT-PCR), fluorescence-activated cell sorting (FACS), plaque assays showed that CRISPR (crRNA) targeting NS3 region...
Altered circulating microRNA (miRNA) profiles have been noted in patients with microbial infections. We compared host serum miRNA levels hand-foot-and-mouth disease (HFMD) caused by enterovirus 71 (EV71) and coxsackievirus 16 (CVA16) as well other infections healthy individuals. Among 664 different miRNAs analyzed using a array, 102 were up-regulated 26 down-regulated sera of enteroviral Expression ten candidate further evaluated quantitative real-time PCR assays. A receiver operating...
Middle East respiratory syndrome coronavirus (MERS-CoV) is continuously spreading and causing severe fatal acute disease in humans. Prophylactic therapeutic strategies are therefore urgently needed to control MERS-CoV infection. Here, we generated a humanized monoclonal antibody (mAb), designated hMS-1, which targeted the receptor-binding domain (RBD) with high affinity. hMS-1 significantly blocked RBD binding its viral receptor, human dipeptidyl peptidase 4 (hDPP4), potently neutralized...
The clinical manifestations and factors associated with the severity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections outside Wuhan are not clearly understood.All laboratory-confirmed cases SARS-Cov-2 infection who were hospitalized monitored in Guangzhou Eighth People's Hospital recruited from January 20 to February 10.A total 275 patients included this study. median patient age was 49 years, 63.6% had exposure Wuhan. virus incubation period 6 days. Fever (70.5%)...
The SARS-coronavirus 2 (SARS-CoV-2) spike (S) protein mediates entry of SARS-CoV-2 into cells expressing the angiotensin-converting enzyme (ACE2). S engages ACE2 through its receptor-binding domain (RBD), an independently folded 197-amino acid fragment 1273-amino S-protein protomer. Antibodies to RBD SARS-CoV (SARS-CoV-1), a closely related coronavirus which emerged in 2002-2003, have been shown potently neutralize SARS-CoV-1 S-protein-mediated entry, and presence anti-RBD antibodies...