A5051902372- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Sperm and Testicular Function
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- Cancer-related molecular mechanisms research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Renal and related cancers
- Brucella: diagnosis, epidemiology, treatment
- Cancer Genomics and Diagnostics
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- Lung Cancer Diagnosis and Treatment
- DNA and Nucleic Acid Chemistry
- Nerve injury and regeneration
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
Nanjing Medical University
2013-2024
Nanjing Forestry University
2023
Institute of Bast Fiber Crops
2023
Sichuan University
2015-2023
West China Hospital of Sichuan University
2015-2023
Liaoning Cancer Hospital & Institute
2018-2023
Hunan Agricultural University
2022
Wuxi No.2 People's Hospital
2018-2022
Hebei University of Science and Technology
2022
Nantong University
2016-2020
Life-long production of spermatozoa depends on spermatogonial stem cells. Spermatogonial cells exist among the most primitive population germ - undifferentiated spermatogonia. Transplantation experiments have demonstrated functional heterogeneity Although spermatogonia can be topographically divided into As (single), Apr (paired), and Aal (aligned) spermatogonia, subdivision this cell using cytological markers would greatly facilitate characterization their functions.In present study, we...
Piwi-interacting RNAs (piRNAs) are essential for silencing of transposable elements in the germline, but their biogenesis is poorly understood. Here we demonstrate that MOV10L1, a germ cell–specific putative RNA helicase, associated with Piwi proteins. Genetic disruption MOV10L1 helicase domain mice renders both MILI and MIWI2 devoid piRNAs. Absence functional piRNA pathway Mov10l1 mutant testes causes loss DNA methylation subsequent derepression retrotransposons cells. The males sterile...
Piwi–piRNA (Piwi-interacting RNA) ribonucleoproteins (piRNPs) enforce retrotransposon silencing, a function critical for preserving the genome integrity of germ cells. The molecular functions most factors that have been genetically implicated in primary piRNA biogenesis are still elusive. Here we show MOV10L1 exhibits 5′-to-3′ directional RNA-unwinding activity vitro and point mutation abolishes this causes failure vivo. We demonstrate selectively binds precursor transcripts is essential...
Piwi-interacting RNAs are a diverse class of small non-coding implicated in the silencing transposable elements and safeguarding genome integrity. In mammals, male germ cells express two genetically developmentally distinct populations piRNAs at pre-pachytene pachytene stages meiosis, respectively. Pre-pachytene mostly derived from retrotransposons required for their silencing. contrast, originate ∼3,000 genomic clusters, biogenesis function remain enigmatic. Here, we report that conditional...
The ability to precisely edit individual bases of bacterial genomes would accelerate the investigation function genes. Here we utilized a nickase Cas9-cytidine deaminase fusion protein direct conversion cytosine thymine within prokaryotic cells, resulting in high mutagenesis frequencies Escherichia coli and Brucella melitensis. Our study suggests that CRISPR/Cas9-guided base-editing is viable alternative approach generate mutant strains.
Mammalian spermatogenesis shows prominent chromatin and transcriptomic switches in germ cells, but it is unclear how such dynamics are controlled. Here we identify RNA helicase DDX43 as an essential regulator of the remodeling process during spermiogenesis. Testis-specific Ddx43 knockout mice show male infertility with defective histone-to-protamine replacement post-meiotic condensation defects. The loss its ATP hydrolysis activity by a missense mutation replicates phenotype global mice....
Significance TATA-binding protein (TBP)-associated factor 7l ( Taf7l ), Taf4b , and TBP-related 2 Trf2 ) were found to be three important atypical testis-specific core promoter recognition factors. However, whether there was any functional cross-talk between them at which stages of spermatogenesis remained uncharacterized. Here we report that − /Y becomes sterile rather than merely subfertile after backcrossing. Importantly, cooperates with but not late direct the transcription key...
Dental pulp stem cells (DPSCs) were widely used as seed in the field of tissue engineering and regenerative medicine, including spinal cord injury (SCI) repair other neuronal degenerative diseases, due to their easy isolation, multiple differentiation potential, low immunogenicity rates rejection during transplantation. Various studies have shown that bFGF can enhance peripheral nerve regeneration after injury, phospho-ERK (p-ERK) activation a major mediator may be involved this process....
The transition from meiotic spermatocytes to postmeiotic haploid germ cells constitutes an essential step in spermatogenesis. epigenomic regulatory mechanisms underlying this remain unclear. Here, we find a prominent transcriptomic switch the late early round spermatids during meiotic-to-postmeiotic transition, which is associated with robust histone acetylation changes across genome. Among deacetylases (HDACs) and acetyltransferases, that HDAC3 selectively expressed stages. Three...
Transcription factors of the Sox protein family contain a DNA-binding HMG box and are key regulators progenitor cell fate. Here, we report that expression Sox30 is restricted to meiotic spermatocytes postmeiotic haploids. mutant males sterile due spermiogenic arrest at early round spermatid stage. Specifically, in absence Sox30, proacrosomic vesicles fail form single acrosomal organelle, spermatids step 2-3. Although most progress through meiosis, accumulation diplotene indicates delayed or...
Dental pulp stem cells (DPSCs), as one type of mesenchymal (MSCs), have the capability self-renewal and multipotency to differentiate into several cell lineages, including osteogenesis, odontoblasts, chondrogenesis, neurogenesis, adipogenesis. It has found that tumor necrosis factor-α (TNF-α) can promote osteogenic differentiation human DPSCs in our previous studies. Other experimentation revealed signal transducer activator transcription 3 (STAT3) underwent a rapid activation both...
The purpose of this experiment was to study the effects fenugreek seed extract (FSE) on growth performance, intestinal morphology, immunity and cecal micro-organisms in yellow-feathered broilers. A total 240 one-day-old male broilers were selected randomly assigned four treatments with 6 replicates per group ten replicate. Started from third day, birds fed basal diet (CON group) or supplemented 30 mg/kg Zinc bacitracin (ZB group), 50 (D-FSE 100 (H-FSE FSE, respectively. lasted for 56 d....
Despite continuous expansion of the RNA binding protein (RBP) world, there is a lack systematic understanding RBPs in mammalian testis, which harbors one most complex tissue transcriptomes. We adapted interactome capture to mouse male germ cells, building an RBP atlas characterized by multiple layers dynamics along spermatogenesis. Trapping RNA-cross-linked peptides showed that glutamic acid-arginine (ER) patch, residue-coevolved polyampholytic element present coiled coils, enhances its host...
MicroRNA (miRNA) is small non-coding RNA with approximate 22 nt in length.Recent studies indicate that miRNAs play significant roles pathogen-host interactions.Brucella organisms are Gram-negative facultative intracellular bacteria cause Brucellosis.Brucella strains infect macrophages and establish chronic infection by altering host life activities including apoptosis autophagy.Here, we report a comprehensive analysis of miRNA expression profiles mock-and Brucella-infected RAW264.7 cells...
With the tremendous increase of publicly available single-cell RNA-sequencing (scRNA-seq) datasets, bioinformatics methods based on gene co-expression network are becoming efficient tools for analyzing scRNA-seq data, improving cell type prediction accuracy and in turn facilitating biological discovery. However, current mainly overall correlation overlook that exists only a subset cells, thus fail to discover certain rare types sensitive batch effect. Here, we developed independent component...
RNA regulation by RNA-binding proteins (RBPs) involve extremely complicated mechanisms. MOV10 and MOV10L1 are two homologous helicases implicated in distinct intracellular pathways. participates specifically Piwi-interacting (piRNA) biogenesis protects mouse male fertility. In contrast, the functional complexity of remains incompletely understood, its role mammalian germline is unknown. Here, we report a study biological molecular functions helicase germ cells. nucleocytoplasmic protein...
Neuropilin-1 (NRP1) is one of the members neuropilin family. It can combine with disparate ligands involved in regulating cell proliferation, apoptosis, and differentiation. The binding NRP1 to Sema3A stimulates osteoblast differentiation through classical Wnt/β-catenin pathway. However, functions dental pulp stem cells (DPSCs) are not clear. aim our study was investigate how controlled odontoblast DPSCs clarified underlying mechanisms. expression increased time-dependent manner along...
MOV10L1 and its paralog MOV10 are evolutionally conserved RNA helicases involved in distinct regulatory pathways. The testis-specific is essential for spermatogenesis PIWI-interacting RNAs biogenesis, whereas ubiquitous multifunctional. Although both proteins have been implied to correlate with G-quadruplex (RG4) vivo, their capabilities binding resolving RG4 respective biological significance remain unclear. Herein, we comprehensively characterize compare the activities of these two on...