A5018900557- Zebrafish Biomedical Research Applications
- RNA Research and Splicing
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- interferon and immune responses
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- RNA regulation and disease
- Retinoids in leukemia and cellular processes
- Genetic and Kidney Cyst Diseases
- Congenital gastrointestinal and neural anomalies
- Hearing, Cochlea, Tinnitus, Genetics
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
Huazhong University of Science and Technology
2018-2024
Institute of Hydrobiology
2024
Chinese Academy of Sciences
2024
Prenatal lethality associated with mouse knockout of Mettl16, a recently identified RNA N6-methyladenosine (m
Abstract Dysfunction of splicing factors often result in abnormal cell differentiation and apoptosis, especially neural tissues. Mutations pre-mRNAs processing factor 31 (PRPF31) cause autosomal dominant retinitis pigmentosa, a progressive retinal degeneration disease. The transcriptome-wide events specifically regulated by PRPF31 their biological roles the development maintenance retina are still unclear. Here, we showed that viability progenitor cells (RPCs) severely perturbed prpf31...
Abstract Mutations in TUB-like protein 1 ( TULP1 ) are associated with severe early-onset retinal degeneration humans. However, the pathogenesis remains largely unknown. There two homologous genes of zebrafish, namely tulp1a and tulp1b . Here, we generated single knockout −/− double tulp1- dKO) models zebrafish. Knockout resulted mislocalization UV cone opsins cones specifically, while rod rod-cone degeneration. In dKO was present all types photoreceptors, observed at a very early age,...
Neural retina leucine zipper ( NRL ) is an essential gene for the fate determination and differentiation of precursor cells into rod photoreceptors in mammals. Mutations are associated with autosomal recessive enhanced S-cone syndrome dominant retinitis pigmentosa. However, exact role Nrl regulating development maintenance zebrafish Danio rerio ), a popular animal model used retinal degeneration regeneration studies, has not been fully determined. In this study, we generated nrl knockout via...
Bietti crystalline dystrophy (BCD) is a progressive retinal degenerative disease primarily characterized by numerous crystal-like deposits and degeneration of pigment epithelium (RPE) photoreceptor cells. CYP4V2 (cytochrome P450 family 4 subfamily V member 2) currently the only disease-causing gene for BCD. We aimed to generate zebrafish model explore functional role in development BCD identify potential therapeutic targets future studies.The cyp4v7 cyp4v8 (homologous genes CYP4V2) knockout...
Mutations that occur in RNA-splicing machinery may contribute to hematopoiesis-related diseases. How splicing factor mutations perturb hematopoiesis, especially the differentiation of erythro-myeloid progenitors (EMPs), remains elusive. Dhx38 is a pre-mRNA splicing-related DEAH box RNA helicase, for which physiological functions and mechanisms during hematopoiesis currently remain unclear. Here, we report exerts broad effect on definitive EMPs as well maintenance hematopoietic stem...
DEAH-Box Helicase 38 (DHX38) is a pre-mRNA splicing factor and also disease-causing gene of autosomal recessive retinitis pigmentosa (arRP).The role DHX38 in the development maintenance retina remains largely unknown.In this study, by using dhx38 knockout zebrafish model, we demonstrated that Dhx38 deficiency causes severe differentiation defects apoptosis retinal progenitor cells (RPCs) through disrupted mitosis increased DNA damage.Furthermore, found significant accumulation R-loops...
Zebrafish is an excellent model for exploring the development of inner ear. Its ear has similar functions to that humans, specifically in maintenance hearing and balance. Mafba a component Maf transcription factor family. It participates multiple biological processes, but its role inner-ear remains poorly understood. In this study, we constructed mafba knockout (mafba-/-) zebrafish using CRISPR/Cas9 technology. The mafba-/- mutant displayed severe impairments, such as enlarged otocysts,...
Inherited retinal diseases (IRDs) affect ∼4.5 million people worldwide. Elusive pathogenic variants in over 280 genes are associated with one or more clinical forms of IRDs. It is necessary to understand the complex interaction among cell types and by constructing a regulatory network. In this study, we attempt establish panoramic expression view cooperative work cells manifestations bases underlying Single-cell RNA sequencing (scRNA-seq) data on retinas from 35 retina samples 3 species...
Introduction: As a congenital and genetically related disease, many single nucleotide polymorphisms (SNPs) have been reported to be associated with the risk of HSCR. Our previous research showed that SNP rs2439302 (NRG1) interacted rs2435357 (RET) increase HSCR development. However, underlying molecular mechanism is still not well understood. Methods: were genotyped in 470 cases. The expression NRG1 RET was investigated colon patients. Knockdown homologs performed zebrafish investigate their...
Abstract The neural retina leucine zipper ( NRL ) is an essential gene for the fate determination and differentiation of rod photoreceptors in mammals. Mutations have been associated with autosomal recessive enhanced S-cone syndrome dominant retinitis pigmentosa. However, exact role Nrl regulating development maintenance zebrafish, a popular animal model used retinal degeneration regeneration studies, has not fully determined. In this study, we generated nrl knockout zebrafish by CRISPR-Cas9...