A5103240150- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Algebraic Geometry and Number Theory
- Bacterial biofilms and quorum sensing
- Enterobacteriaceae and Cronobacter Research
- Infections and bacterial resistance
- Genomics and Phylogenetic Studies
- Advanced Algebra and Geometry
- Geometry and complex manifolds
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Bacterial Identification and Susceptibility Testing
- Escherichia coli research studies
- Planarian Biology and Electrostimulation
- Aquaculture disease management and microbiota
- Plant Pathogenic Bacteria Studies
- Bacterial Genetics and Biotechnology
- Analytic Number Theory Research
- Geometric Analysis and Curvature Flows
- Complex Network Analysis Techniques
- Antibiotics Pharmacokinetics and Efficacy
- Salmonella and Campylobacter epidemiology
- Graph theory and applications
- Neuroscience and Neural Engineering
- Antimicrobial Resistance in Staphylococcus
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2016-2025
National Clinical Research Center for Digestive Diseases
2024-2025
First Affiliated Hospital Zhejiang University
2020-2025
Zhejiang University
2016-2025
Westlake University
2019-2025
Xinjiang Institute of Ecology and Geography
2021-2025
Chinese Academy of Sciences
2021-2025
University of Chinese Academy of Sciences
2021-2025
First Affiliated Hospital of Wannan Medical College
2024
Wenzhou Medical University
2020-2023
In this study, we characterized the miR482 family in cotton using existing small RNA datasets and recently released draft genome sequence of Gossypium raimondii, a diploid species whose progenitor is putative contributor Dt (representing D tetraploid) cultivated tetraploid G. hirsutum barbadense. Of three ghr-miR482 members reported hirsutum, ghr-miR482a has no homolog ghr-miR482b ghr-miR482c each single raimondii. Gra-miR482d five homologous loci (gra-miR482d, f-i) raimondii also exists...
Transmissible colistin resistance mediated by the mcr gene has been reported worldwide, but clinical isolates of mcr-negative colistin-resistant Escherichia coli are rarely reported. The aim this study was to evaluate mechanism among mcr-positive and E. performing a molecular epidemiological surveillance. For first time ever, we show nearly same isolation ratio for (47.5 52.5%, respectively), with no demonstrable nosocomial transmission. We provide evidence prevalence IncX4 plasmid its high...
We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues micron-scale (4 × 4 10 μm3) to submicron-scale (0.3 0.3 1 spatial resolution. The resolving ability is improved sub-100 nm (70 70 200 nm3) via expansion. uses sheets achieve higher resolution and better optical sectioning than conventional microscopes. illumination phase modulated adjust the position intensity profile based on desired speed keep aligned....
Background: Klebsiella pneumoniae has been the leading causative pathogen for adult bacterial meningitis in several Asian countries. The clinical and microbiological characteristics of K. mainland China are still unknown. Materials methods: data patients with from January 2011 to July 2017 a tertiary hospital were retrospectively evaluated. isolates tested antibiotic-resistance genes, virulence-associated molecular subtypes. Hypervirulent (hvKP) was defined as presence pLVPK-like virulence...
Potentilla L. and Dasiphora are predominantly perennial herbs, occasionally manifesting as annuals or shrubs, primarily found in the northern temperate zone. However, taxonomic classification within this group remains contentious, particularly regarding genus boundaries species delineation. Therefore, study sequenced analyzed complete plastid genomes of 19 from Dasiphora, comparing them with five previously published sequences. Our objectives included reconstructing phylogenetic...
Blood flow and mechanical forces in the ventricle are implicated cardiac development trabeculation. However, mechanisms of mechanotransduction remain elusive. This is due part to challenges associated with accurately quantifying developing heart. We present a novel computational framework simulate hemodynamics zebrafish embryos by coupling 4-D light sheet imaging stabilized finite element solver, extract time-dependent stimuli data. employ deformable image registration methods segment motion...
Abstract Light Sheet Fluorescence Microscopy (LSFM) enables multi-dimensional and multi-scale imaging via illuminating specimens with a separate thin sheet of laser. It allows rapid plane illumination for reduced photo-damage superior axial resolution contrast. We hereby demonstrate cardiac LSFM (c-LSFM) to assess the functional architecture zebrafish embryos retrospective synchronization algorithm four-dimensional reconstruction (3-D space + time). By combining our approach tissue clearing...
The emergence and spread of the mobile colistin resistance gene (mcr-1) has become a major global public health concern. So far, this been widely detected in food animals, pets, food, humans. However, there is little information on contamination mcr-1-containing bacteria farming soils. In August 2016, survey ESBL-producing Escherichia coli isolated from soils was conducted Shandong Province, China. We observed 12 53 (22.6%) Enterobacteriaceae isolates soil. Six mcr-1-positive E. strains...
Abstract The giant grouper ( Epinephelus lanceolatus ) is the largest coral reef teleost, with a native range that spans temperate and tropical waters in Pacific Indian Oceans. It cultured artificially used as breeding species aquaculture due to its rapid growth rate. Here we report genome assembled at chromosome scale from sequences generated using Illumina high‐throughput chromatin conformation capture (Hi‐C) technology. assembly comprised 1.086 Gb, 98.4% of scaffold anchored into 24...
The emergence and spread of carbapenem-resistant hypervirulent Klebsiella pneumoniae sequence type 11 (ST11-CR-HvKP) in China are a great concern the public health community. However, underlying mechanism that enables its wide dissemination remains unclear. Here, we investigated prevalence carriage carbapenemase-producing Enterobacteriaceae (CPE) among inpatients with diarrhea teaching hospital over 1 year to identify ST11-CR-HvKP reservoirs understand genetic background plasmid profiles...
Abstract Moso bamboo ( Phyllostachys edulis ) is an economically and ecologically important nontimber forestry species. Further development of this species as a sustainable resource has been hindered by lack population genome information. Here, we report moso genomic variation atlas 5.45 million single-nucleotide polymorphisms (SNPs) from whole-genome resequencing 427 individuals covering 15 representative geographic areas. We uncover low genetic diversity, high genotype heterozygosity,...
Abstract The leopard coral grouper, Plectropomus leopardus , belonging to the family Epinephelinae, is a carnivorous reef fish widely distributed in tropical and subtropical waters of Indo‐Pacific. Due its appealing body appearance delicious taste, P. has become popular commercial for aquaculture many countries. However, lack genomic molecular resources hindered study biology breeding programmes. Here we report de novo sequencing assembly genome using combination 10 × Genomics,...
Tigecycline is one of the last-resort antibiotics to treat severe infections. Recently, tigecycline resistance has sporadically emerged with an increasing trend, and Tet(X) family represents a new mechanism tigecycline. In this study, novel chromosome-encoded gene, tet(X14), was identified in tigecycline-resistant colistin-resistant Empedobacter stercoris strain ES183 recovered from pig fecal sample China. Tet(X14) shows 67.14-96.39% sequence identity other variants [Tet(X) Tet(X13)]....
The emergence of carbapenem-resistant Enterobacteriaceae strains has posed a severe threat to public health in recent years. mobile elements carrying the New Delhi metallo-β-lactqtamase (NDM) gene have been regarded as major mechanism leading rapid increase isolated from clinics and animals.We describe an NDM-5-producing Escherichia coli strain, ECCRA-119 (sequence type 156 [ST156]), poultry farm Zhejiang, China. is multidrug-resistant (MDR) isolate that exhibited resistance 27 antimicrobial...
We previously described the discovery of two Escherichia coli isolates (EC1002 and EC2474) co-harbouring mcr-1 bla NDM-1 genes, which were recovered from bloodstream infection in China. More importantly, these antibiotic resistance genes located on different plasmids signaling potential spread pandrug-resistant bacteria. Here, complete genome sequences both determined using Pacbio RS II Illumina HiSeq2000 systems. The EC1002 consists a 5,177,501 base pair chromosome four circular plasmids,...
To investigate the prevalence and molecular characteristics of ESBL-producing Escherichia coli (ESBL-EC) in faecal samples from dairy cows China.In total, 651 were collected distributed among 10 provinces China. Potential ESBL-EC isolates cultured on selective medium. The clonal relatedness was assessed using MLST. WGS conducted 3 mcr-positive 14 additional randomly selected isolates. Southern blot, S1-PFGE conjugation performed for mcr-1-carrying genetic environment pMCR-JLF4 plasmid also...
To examine the characteristics and spatiotemporal changes in phenotypes genotypes of extended-spectrum β-lactamases (ESBLs) Escherichia coli strains isolated from bloodstream infections (BSIs) across China between 2014 2021. 983 ESBL-positive E. were collected BSIs 66 hospitals different geographic regions to The phenotypic confirmation ESBL was performed through double-disc diffusion method. genetic type determined using polymerase chain reaction (PCR) followed by DNA sequencing. Between...
Abstract Artemisia L. is one of the most diverse genera in Asteraceae, widely used agriculture and medicine, with a giant range complicated taxa. The task establishing phylogeny difficulties owing to highly similar morphological characters. East Asia, biodiversity hotspot major usage area for Artemisia, has received limited attention. Here, we collected 71 species (two subspecies, 94 samples) its allies from Asia neighbouring regions, combined public databases, ensuring representation all...