A5101487886- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Digital Holography and Microscopy
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Optical Coherence Tomography Applications
- Planarian Biology and Electrostimulation
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Advanced Electron Microscopy Techniques and Applications
- Plant and Biological Electrophysiology Studies
- Neurogenesis and neuroplasticity mechanisms
- Photoreceptor and optogenetics research
- Biocrusts and Microbial Ecology
- Advanced Optical Sensing Technologies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Retinal Development and Disorders
- Nanoplatforms for cancer theranostics
- Nerve injury and regeneration
- Advanced Neuroimaging Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Near-Field Optical Microscopy
- Heme Oxygenase-1 and Carbon Monoxide
- Caveolin-1 and cellular processes
- Reproductive Biology and Fertility
Westlake University
2019-2025
Institute for Advanced Study
2024-2025
Zhejiang University
2024
University of California, Los Angeles
2021-2024
Fudan University
2024
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2019-2022
Intelligent Light (United States)
2017-2018
Stony Brook University
2014-2016
Janelia Research Campus
2011-2015
Howard Hughes Medical Institute
2011-2015
Tissue clearing technique enables visualization of opaque organs and tissues in 3-dimensions (3-D) by turning tissue transparent. Current methods are restricted limited types that can be cleared with each individual protocol, which inevitably led to the presence blind-spots within whole body or parts imaging. Hard including bones teeth still most difficult cleared. In addition, loss endogenous fluorescence remains a major concern for solvent-based methods. Here, we developed polyethylene...
Cytotoxic T lymphocytes (CTLs) use polarized secretion to rapidly destroy virally infected and tumor cells. To understand the temporal relationships between key events leading secretion, we used high-resolution 4D imaging. CTLs approached targets with actin-rich projections at edge, creating an initially actin-enriched contact rearward-flowing actin. Within 1 min, cortical actin reduced across synapse, cell receptors (TCRs) clustered centrally form central supramolecular activation cluster...
Recent methods have revealed that cells on planar substrates exert both shear (in-plane) and normal (out-of-plane) tractions against the extracellular matrix (ECM). However, location origin of with respect to adhesive cytoskeletal elements not been elucidated. We developed a high-spatiotemporal-resolution, multidimensional (2.5D) traction force microscopy measure model full 3D nature cellular forces 2D surfaces. show are centered under elongated focal adhesions whereas upward downward...
Apical constriction changes cell shapes, driving critical morphogenetic events, including gastrulation in diverse organisms and neural tube closure vertebrates. is thought to be triggered by contraction of apical actomyosin networks. We found that contractions began before shape both Caenorhabitis elegans Drosophila. In C. elegans, networks were initially dynamic, contracting generating cortical tension without substantial shrinking surfaces. cell-cell contact zones only later moved...
Selective Plane Illumination Microscopy (SPIM) is attractive for its ability to acquire 3D images with high spatial resolution, good optical sectioning capability and imaging speed. However, tradeoffs have be made when a large field of view (FOV) required, results in lower axial resolution or worse capability. Here, we present novel method by SPIM that capable maintain within FOV. Instead trying generate uniformly thick excitation light sheet, the tiles relative small sheet quickly multiple...
Abstract Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet microscopy (TLS-SPIM) with real-time optimization was developed to respond challenge. It improves ability SPIM resolving complex structures and optimizes performance by using a adjustable tiling light sheet creating flexible compromise between spatial temporal resolution. We demonstrate TLS-SPIM cellular subcellular...
Vinculin is filamentous (F)-actin-binding protein enriched in integrin-based adhesions to the extracellular matrix (ECM). Whereas studies 2-dimensional (2D) tissue culture models have suggested that vinculin negatively regulates cell migration by promoting cytoskeleton-ECM coupling strengthen and stabilize adhesions, its role regulating more physiologic, 3-dimensional (3D) environments unclear. To address of 3D migration, we analyzed morphodynamics, ECM remodeling primary murine embryonic...
Optical imaging has served as a primary method to collect information about biosystems across scales-from functionalities of tissues morphological structures cells and even at biomolecular levels. However, adequately characterize complex biosystem, an system with number resolvable points, referred space-bandwidth product (SBP), in excess one billion is typically needed. Since gigapixel-scale far exceeds the capacity current optical imagers, compromises must be made obtain either low spatial...
We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues micron-scale (4 × 4 10 μm3) to submicron-scale (0.3 0.3 1 spatial resolution. The resolving ability is improved sub-100 nm (70 70 200 nm3) via expansion. uses sheets achieve higher resolution and better optical sectioning than conventional microscopes. illumination phase modulated adjust the position intensity profile based on desired speed keep aligned....
Light-sheet fluorescence microscopy (LSFM) introduces fast scanning of biological phenomena with deep photon penetration and minimal phototoxicity. This advancement represents a significant shift in 3-D imaging large-scale tissues 4-D (space + time) small live animals. The large data associated LSFM requires efficient acquisition analysis the use artificial intelligence (AI)/machine learning (ML) algorithms. To this end, AI/ML-directed is an emerging area for multi-organ tumor diagnostics....
<title>Abstract</title> Spinal motoneurons control muscle fibers contraction and drive all motor behaviors in vertebrates. Although spinal share the fundamental role of innervating fibers, they exhibit remarkable diversity that reflects their specific identities. Defining morphological changes during postnatal development is critical for elucidating this diversity. However, our understanding three-dimensional (3D) morphology at these stages remains limited, largely due to lack...
The intricate coordination of the neural network in planarian growth and regeneration has remained largely unrevealed, partly due to challenges imaging central nervous system (CNS) three dimensions (3D) with high resolution within a reasonable timeframe. To address this gap systematic CNS planarians, we adopted high-resolution, nanoscale by combining tissue expansion tiling light-sheet microscopy, achieving up 4-fold linear expansion. Using an automatic 3D cell segmentation pipeline,...
The intricate coordination of the neural network in planarian growth and regeneration has remained largely unrevealed, partly due to challenges imaging CNS three dimensions (3D) with high resolution within a reasonable timeframe. To address this gap systematic planarians, we adopted high-resolution, nanoscale by combining tissue expansion tiling light-sheet microscopy, achieving up fourfold linear expansion. Using an automatic 3D cell segmentation pipeline, quantitatively profiled neurons...
Selective plane illumination microscopy (SPIM) allows rapid 3D live fluorescence imaging on biological specimens with high spatial resolution, good optical sectioning capability and minimal photobleaching phototoxic effect. SPIM gains its advantage by confining the excitation light near detection focal plane, performance is determined ability to create a thin, large uniform sheet. Several methods have been developed such an sheet for SPIM. However, each method has own strengths weaknesses,...
We present a novel method used to implement tiling lattice light sheets (LLS) in sheet microscopy (LLSM) on regular LLS microscopes without changing the microscope hardware. A is tiled by applying binary phase maps acquired from off-center cross-sections of corresponding optical SLM microscopes, which thin can be image large specimens while maintaining 3D imaging ability entire field view. investigate via numerical simulations and experiments, demonstrate fluorescent particles embedded...
Descending tracts carry motor signals from the brain to spinal cord. However, few previous studies show full view of long a 3D perspective. In this study, we have followed five less well-known that project midbrain, hindbrain, and cerebellum mouse cord, using tissue clearing method in combination with tiling light sheet microscopy. By tracing axons found several notable features: among collateral "sister" branches occurred only originating cerebellospinal tracts; trigeminal nucleus crossed...
Here, we describe a protocol to construct, calibrate, and operate versatile tiling light sheet microscope for imaging cleared tissues. The uses adjustable sheets achieve higher spatial resolution better optical sectioning ability than conventional microscopes image tissues with the cellular subcellular resolution. It is compatible all tissue clearing methods aligned semiautomatically through phase modulation of illumination light. For complete details on use execution this protocol, please...
We have previously developed a line-scanned dual-axis confocal (LS-DAC) microscope with subcellular resolution suitable for high-frame-rate diagnostic imaging at shallow depths. Due to the loss of confocality along one dimension, contrast (signal-to-background ratio) LS-DAC is deteriorated compared point-scanned DAC microscope. However, by using sCMOS camera detection, short oblique light-sheet imaged each scanned position. Therefore, scanning light sheet in only thin 3D volume imaged. Both...