A5088428348- Biofuel production and bioconversion
- Sugarcane Cultivation and Processing
- Plant Disease Resistance and Genetics
- Genetics and Plant Breeding
- Genetic diversity and population structure
- Fungal and yeast genetics research
- Plant and animal studies
- Plant pathogens and resistance mechanisms
- Plant nutrient uptake and metabolism
- Plant Taxonomy and Phylogenetics
- Agricultural pest management studies
- Wheat and Barley Genetics and Pathology
- Soybean genetics and cultivation
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Plant Diversity and Evolution
- Genetic Mapping and Diversity in Plants and Animals
- Microbial Metabolic Engineering and Bioproduction
- Horticultural and Viticultural Research
- Fish Ecology and Management Studies
- Genetic and Environmental Crop Studies
- Natural Products and Biological Research
- Fern and Epiphyte Biology
Universidade Estadual de Campinas (UNICAMP)
2014-2024
Hospital de Clínicas da Unicamp
2011
Many plant species of great economic value (e.g., potato, wheat, cotton and sugarcane) are polyploids. Despite the essential roles autopolyploid plants in human activities, our genetic understanding these is still poor. Recent progress instrumentation biochemical manipulation has led to accumulation an incredible amount genomic data. In this study, we demonstrate for first time a successful analysis highly polyploid genome (sugarcane) by quantitative single-nucleotide polymorphism (SNP)...
Abstract This article documents the addition of 220 microsatellite marker loci to Molecular Ecology Resources Database. Loci were developed for following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco,...
The protein kinase (PK) superfamily is one of the largest superfamilies in plants and core regulator cellular signaling. Despite this substantial importance, kinomes sugarcane sorghum have not been profiled. Here, we identified profiled complete polyploid Saccharum spontaneum (Ssp) Sorghum bicolor (Sbi), a close diploid relative. Sbi kinome was composed 1,210 PKs; for Ssp, 2,919 PKs when disregarding duplications allelic copies, these were related to 1,345 representative gene models. Ssp...
Whole genome duplication has played an important role in plant evolution and diversification. Sugarcane is crop with a complex hybrid polyploid genome, for which the process of adaptation to polyploidy still poorly understood. In order improve our knowledge about sugarcane homo/homeologous gene expression balance, we sequenced analyzed 27 BACs (Bacterial Artificial Chromosome) R570 cultivar, containing putative single-copy genes LFY (seven haplotypes), PHYC (four TOR haplotypes). Comparative...
Sugarcane (Saccharum spp.) is highly polyploid and aneuploid. Modern cultivars are derived from hybridization between S. officinarum spontaneum. This combination results in a genome exhibiting variable ploidy among different loci, huge size (approximately 10 Gb) high content of repetitive regions. An approach using genomic, transcriptomic genetic mapping can improve our knowledge the behavior genetics sugarcane. The hypothetical HP600 Centromere Protein C (CENP-C) genes sugarcane were used...
Trichoderma harzianum IOC-3844 secretes high levels of cellulolytic-active enzymes and is therefore a promising strain for use in biotechnological applications second-generation bioethanol production. However, the T. biomass degradation mechanism has not been well explored at genetic level. The present work investigates six genomic regions (~150 kbp each) this fungus that are enriched with genes related to conversion. A BAC library consisting 5,760 clones was constructed, an average insert...
Sugarcane exhibits a complex genome mainly due to its aneuploid nature and high ploidy level, sequencing of poses great challenge. Closely related species with well-assembled annotated genomes can be used help assemble genomes. Here, stable quantitative trait locus (QTL) sugar accumulation in sorghum was successfully transferred the sugarcane genome. Gene sequences this QTL were identified silico from transcriptome data, molecular markers based on these developed select bacterial artificial...
The objective of this work was to evaluate a set microsatellite markers for varietal identification and characterization the most widespread potato cultivars in Brazil. DNA from 14 genotyped using alleles were scored silver-stained polyacrylamide gel. Twenty-four evaluated, only one locus monomorphic. Based on band patterns, two microsatellites that able identify differentiate all examined obtained.
Orphan genes (OGs) are protein-coding that restricted to particular clades or species and lack homology with from other organisms, making their biological functions difficult predict. OGs can rapidly originate become functional; consequently, they may support rapid adaptation environmental changes. Extensive spread of mobile elements whole-genome duplication occurred in the Saccharum group, which have contributed origin diversification sugarcane genome. Here, we identified characterized...
The objective of this work was to develop new microsatellite markers in common bean. Ninety nine microsatelitte loci were developed from a enriched library for (CT)8 and (GT)8 motifs, CAL-143 line. majority sequences (51%) related cellular metabolism. remaining associated transcription functions. Only 17.2% the presented some level similarity with other plant species genes.
The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in food, fodder, soil conservation, crop-livestock integrated systems, reclaiming degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate genetic diversity 77 genotypes selected from germplasm collections at Embrapa Cattle-Southeast and, evaluate their transferability Phaseolus vulgaris Vigna unguiculata species. number alleles per locus ranged 2...
The objectives of this work were to adapt the fluorescent labeling polymerase chain reaction (PCR) technique using M13 universal primer for genotyping purposes, and present a new set microsatellite markers common bean (Phaseolus vulgaris L.).A large population (380 lines) was used genotyping.PCR method showed be very efficient multiplex analysis, providing lower costs saving time, thus increasing quality analysis.A 50 microsatellites developed from an enriched library derived cultivar...
With 1 figure and table Abstract The Guineagrass ( Panicum maximum Jacq.) is one of the most important tropical forage grasses, but genetic knowledge tools regarding this species are still limited. Therefore, 20 novel polymorphic microsatellite markers were developed, validated, employed in estimating relationships among 25 P. genotypes selected from a Brazilian germplasm collection. In addition, they tested for cross‐species amplification four other grass species. number alleles observed...
Abstract Background Unveiling fungal genome structure and function reveals the potential biotechnological use of fungi. Trichoderma harzianum is a powerful CAZyme-producing fungus. We studied genomic regions in T. IOC3844 containing CAZyme genes, transcription factors transporters. Results used bioinformatics tools to mine for genomics, transcriptomics, exoproteomics data coexpression networks. The DNA was sequenced by PacBio SMRT technology multiomics analysis integration. In total, 1676...
Multiple genes in sugarcane control sucrose accumulation and the biosynthesis of cell wall components; however, it is unclear how these are expressed its apical culms. To better understand this process, we sequenced mRNA from +1 stem internodes collected four genotypes with different concentrations soluble solids. Culms were at time points, ranging six to 12-month-old plants. Here show differentially related metabolism biosynthesis, including encoding invertases, synthase cellulose synthase....
In the Clusia criuva Cambess. species complex, two subspecies C. subsp. parviflora Vesque and can only be distinguished on basis of stamen/staminode morphology geographical occurrence. Despite being recently restructured, taxonomic relationships in this complex remain unclear. Therefore, to illuminate evolutionary mechanisms involved diversification these lineages we investigated their population structure, phylogeographical niche distribution patterns using plastid nuclear microsatellites...