A5048775238- Influenza Virus Research Studies
- Bacteriophages and microbial interactions
- Virology and Viral Diseases
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Enzyme Structure and Function
- Animal Virus Infections Studies
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Glycosylation and Glycoproteins Research
- Herpesvirus Infections and Treatments
- Protein Structure and Dynamics
- interferon and immune responses
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- Immune Response and Inflammation
- Biochemical and Molecular Research
- Animal Disease Management and Epidemiology
- Microbial Metabolic Engineering and Bioproduction
Georgia State University
2015-2025
Wuhan No.1 Hospital
2024-2025
Hainan Provincial Academy of Marine Fisheries and Aquaculture
2023-2025
Shanxi Medical University
2019-2024
Second Hospital of Shanxi Medical University
2019-2024
Southwest Petroleum University
2024
PetroChina Southwest Oil and Gas Field Company (China)
2024
Mae Fah Luang University
2023
Xinjiang Agricultural University
2023
Tongji Hospital
2022
The three-dimensional atomic structure of a single-stranded DNA virus has been determined. Infectious virions canine parvovirus contain 60 protein subunits that are predominantly VP-2. central structural motif VP-2 the same topology (an eight-stranded antiparallel beta barrel) as found in many other icosahedral viruses but represents only about one-third capsid protein. There is 22 angstrom (A) long protrusion on threefold axes, 15 A deep canyon circulating each five cylindrical structures...
WIN 51711 and 52084 are structurally related, antiviral compounds that inhibit the replication of rhino (common cold) viruses related picornaviruses. They prevent pH-mediated uncoating viral RNA. The consist a 3-methylisoxazole group inserts itself into hydrophobic interior VP1 beta-barrel, connecting seven-membered aliphatic chain, 4-oxazolinylphenoxy (OP) covers entrance to an ion channel in floor "canyon." Viral disassembly may be inhibited by preventing collapse pocket or blocking flow...
The structure of Mengo virus, a representative member the cardio picornaviruses, is substantially different from structures rhino- and polioviruses. virus was solved with use human rhinovirus 14 as an 8 Å resolution structural approximation. Phase information then extended to 3 by icosahedral symmetry. This procedure gives promise that many other also can be determined without isomorphous replacement technique. Although organization major capsid proteins VP1, VP2, VP3 essentially same in...
Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic and involved in multiple functions including transcription, replication, assembly. We have determined 2.9 angstrom structure of complex containing 10 molecules N protein 90 bases RNA. The tightly sequestered cavity at interface between two lobes protein. This serves to protect absence polynucleotide synthesis. For be accessed, some conformational change should necessary.
Dendritic mRNA transport and local translation at individual potentiated synapses may represent an elegant way to form synaptic memory. Recently, we characterized Staufen, a double-stranded RNA-binding protein, in rat hippocampal neurons showed its presence large RNA-containing granules, which colocalize with microtubules dendrites. In this paper, transiently transfect human Staufen-green fluorescent protein (GFP) find granules the somatodendritic domain of these cells. Human Stau-GFP show...
Staufen (Stau) is a double-stranded RNA (dsRNA)-binding protein involved in mRNA transport and localization Drosophila.To understand the molecular mechanisms of mammals, we cloned human (hStau) mouse (mStau)staufen cDNAs. In humans, four transcripts arise by differential splicing Stau gene code for two proteins with different N-terminal extremities. vitro, hStau mStau bind dsRNA via each full-length dsRNA-binding domains tubulin region similar to microtubule-binding domain MAP-1B, suggesting...
Vesicular stomatitis virus (VSV) is a bullet-shaped rhabdovirus and model system of negative-strand RNA viruses. Through direct visualization by means cryo-electron microscopy, we show that each virion contains two nested, left-handed helices: an outer helix matrix protein M inner nucleoprotein N RNA. has hub domain with four contact sites link to neighboring subunits, providing rigidity clamping adjacent turns the nucleocapsid. Side-by-side interactions between subunits are critical for...
Mature picornaviral proteins are derived by progressive, posttranslational cleavage of a precursor polyprotein. These cleavages play role in the control virus functions. Although processed termini separated as much 75 A native capsid, fold and arrangement polypeptide chains protomer before proteolysis likely to be similar that found mature virus. The three-dimensional structures rhinovirus Mengo suggest sites within protomeric structurally flexible regions. final proteolytic processing...
The binding to human rhinovirus 14 of a series eight antiviral agents that inhibit picornaviral uncoating after entry into host cells has been characterized crystallographically. All these bind the same hydrophobic pocket within viral protein VP1 beta-barrel structure, although orientation and position each compound was found differ. compounds cause shell be less flexible, thereby inhibiting disassembly. Although potency varies by 120-fold, they all induce conformational changes on virion....
Metabolic reprogramming of cancer cells is essential for tumorigenesis in which pyruvate kinase M2 (PKM2), the low activity isoform kinase, plays a critical role. Herein, we describe identification nature-product-derived micheliolide (MCL) that selectively activates PKM2 through covalent binding at residue cysteine424 (C424), not contained PKM1. This interaction promotes more tetramer formation, inhibits lysine433 (K433) acetylation, and influences translocation into nucleus. In addition,...
Human cytomegalovirus (HCMV) enters host by glycoprotein B (gB)-mediated membrane fusion upon receptor-binding to gH/gL-related complexes, causing devastating diseases such as birth defects. Although an X-ray crystal structure of the recombinant gB ectodomain at postfusion conformation is available, structures prefusion and its complex with gH/gL on viral envelope remain elusive. Here, we demonstrate utility cryo electron tomography (cryoET) energy filtering cutting-edge technologies Volta...
Evidence suggests that increased microRNA-155 (miR-155) expression in immune cells enhances antitumor responses. However, given the reported association of miR-155 with tumorigenesis various cancers, a debate is provoked on whether oncogenic or tumor suppressive. We aimed to interrogate impact expression, particularly cancer cell-derived miR-155, immunity breast cancer. performed bioinformatic analysis human databases, murine experiments, and specimen examination. revealed higher levels...
Genome-based analysis is crucial in monitoring antibiotic-resistant bacteria (ARB)and antibiotic-resistance genes (ARGs). Short-read sequencing typically used to obtain incomplete draft genomes, while long-read can genomes of multidrug resistance (MDR) plasmids and track the transmission plasmid-borne antimicrobial bacteria. However, suffers from low-accuracy base calling, short-read often required improve genome accuracy. This increases costs turnaround time.In this study, a novel ONT...
Abstract Background Intracerebral hemorrhage (ICH) is a common stroke type with high morbidity and mortality. There are mainly three surgical methods for treating ICH. Unfortunately, thus far, no specific method has been proven to be the most effective. We carried out this study investigate whether minimally invasive surgeries endoscopic surgery or stereotactic aspiration (frameless navigated aspiration) will improve functional outcomes in patients supratentorial ICH compared small-bone flap...
ABSTRACT Human Staufen (hStau), a double-stranded RNA (dsRNA)-binding protein that is involved in mRNA transport, incorporated human immunodeficiency virus type 1 (HIV-1) and other retroviruses, including HIV-2 Moloney murine leukemia virus. Sucrose Optiprep gradient analyses reveal cosedimentation of hStau with purified HIV-1, while subtilisin assays demonstrate it internalized. incorporation HIV-1 selective, dependent on an intact functional dsRNA-binding domain, quantitatively correlates...
ABSTRACT Influenza A viruses possess two glycoprotein spikes on the virion surface: hemagglutinin (HA), which binds to oligosaccharides containing terminal sialic acid, and neuraminidase (NA), removes acid from oligosaccharides. Hence, interplay between these receptor-binding receptor-destroying functions assumes major importance in viral replication. In contrast well-characterized role of HA host range restriction influenza viruses, there is only limited information NA substrate specificity...
The negative-strand RNA viruses (NSRVs) are unique because their nucleocapsid, not the naked RNA, is active template for transcription and replication. viral polymerase of nonsegmented NSRVs contains a large catalytic subunit (L) nonenzymatic cofactor, phosphoprotein (P). Insight into how P delivers complex to nucleocapsid has long been pursued by reverse genetics biochemical approaches. Here, we present X-ray crystal structure C-terminal domain vesicular stomatitis virus, prototypic NSRV,...
Inherently unstable mRNAs contain AU-rich elements (AREs) in the 3′ untranslated regions. Expression of ARE-containing type I interferon transcripts is robustly induced upon viral infection and rapidly shut off thereafter. Their transient accumulation partly mediated through posttranscriptional regulation. Here we show that mouse embryonic fibroblasts derived from knockout mice deficient KH-type splicing regulatory protein (KSRP), an RNA-binding required for ARE-mediated mRNA decay, produce...
All orthobunyaviruses possess three genome segments of single-stranded negative sense RNA that are encapsidated with the virus-encoded nucleocapsid (N) protein to form a ribonucleoprotein (RNP) complex, which is uncharacterized at high resolution. We report crystal structure both Bunyamwera virus (BUNV) N–RNA complex and unbound Schmallenberg (SBV) N protein, resolutions 3.20 2.75 Å, respectively. Both proteins crystallized as ring-like tetramers exhibit degree structural similarity despite...
Significance Respiratory syncytial virus (RSV) causes major disease in pediatric and elderly patients, urging the development of efficacious therapeutics. This study establishes a recombinant RSV reporter strain for drug discovery identifies an entry inhibitor class targeting viral fusion (F) protein. Biochemical, structural, functional characterization spotlights two microdomains governing conformational stability prefusion F. Mutations these domains cause broad cross-resistance against...