A5045675715- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Chemical Synthesis and Analysis
- Diet, Metabolism, and Disease
- Monoclonal and Polyclonal Antibodies Research
- Microfluidic and Capillary Electrophoresis Applications
- Nanopore and Nanochannel Transport Studies
- RNA and protein synthesis mechanisms
- Nanoparticle-Based Drug Delivery
- Peptidase Inhibition and Analysis
- Galectins and Cancer Biology
- Proteoglycans and glycosaminoglycans research
- Cancer Research and Treatments
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Advanced Drug Delivery Systems
- Advanced Proteomics Techniques and Applications
- Genomics and Phylogenetic Studies
- Pancreatic function and diabetes
- Catalysis for Biomass Conversion
- Advanced Biosensing Techniques and Applications
- Adenosine and Purinergic Signaling
- Studies on Chitinases and Chitosanases
- Histone Deacetylase Inhibitors Research
- Enzyme Production and Characterization
University of Chinese Academy of Sciences
2022-2025
Shanghai Institute of Materia Medica
2020-2025
Chinese Academy of Sciences
2020-2025
Nanjing University of Chinese Medicine
2022-2025
National Center for Drug Screening
2023
Georgia State University
2013-2020
AID Atlanta
2016
Tianjin Economic-Technological Development Area
2011
Nankai University
2011
Ministry of Education of the People's Republic of China
2011
The ability of nanoparticles to target tumors and enable site-specific drug release provides a unique system for the delivery effective therapy with reduced toxic side effects. In this study, we used mesoporous silica (MSN) fabricate targeted that is responsive hyaluronidase (HAase). Following engraftment desthiobiotin onto surface MSN, streptavidin complex was generated, which functionalized biotin-modified hyaluronic acid (HA) controlled at cancer cells expressing HAase. Various...
Metabolic reprogramming of cancer cells is essential for tumorigenesis in which pyruvate kinase M2 (PKM2), the low activity isoform kinase, plays a critical role. Herein, we describe identification nature-product-derived micheliolide (MCL) that selectively activates PKM2 through covalent binding at residue cysteine424 (C424), not contained PKM1. This interaction promotes more tetramer formation, inhibits lysine433 (K433) acetylation, and influences translocation into nucleus. In addition,...
Human milk oligosaccharides (HMOs) are a family of diverse unconjugated glycans that exist in human as one the major components. Characterization, quantification, and biofunctional studies HMOs remain great challenge due to their diversity complexity. The accessibility homogeneous HMO library is essential solve these issues which have beset academia for several decades. In this study, an efficient chemoenzymatic strategy, namely core synthesis/enzymatic extension (CSEE), rapid production was...
Abstract As the most abundant organic substances in nature, carbohydrates are essential for life. Understanding how regulate proteins physiological and pathological processes presents opportunities to address crucial biological problems develop new therapeutics. However, diversity complexity of pose a challenge experimentally identifying sites where bind act on proteins. Here, we introduce deep learning model, DeepGlycanSite, capable accurately predicting carbohydrate-binding given protein...
Disialosyl globopentaosylceramide (DSGb5) is a tumor-associated complex glycosphingolipid. However, the accessibility of structurally well-defined DSGb5 for precise biological functional studies remains challenging. Herein, we describe first total synthesis glycolipid by an efficient chemoenzymatic approach. A Gb5 pentasaccharide-sphingosine was chemically synthesized convergent and stereocontrolled [2 + 3] method using oxazoline disaccharide donor to exclusively form β-anomeric linkage....
The crucial roles that glycans play in biological systems are determined by their structures. However, the analysis of glycan structures still has numerous bottlenecks due to inherent complexities. nanopore technology emerged as a powerful sensor for DNA sequencing and peptide detection. This significant impact on development related research area. Currently, nanopores beginning be applied detection simple glycans, but complex this is challenging. Here, we designed an engineered α-hemolysin...
Abstract For decades, researchers have endeavored to develop a general automated system synthesize oligosaccharides that is comparable the preparation of oligonucleotides and oligopeptides by commercially available machines. Inspired success oligosaccharide synthesis through chemical glycosylation, fully reported for enzymatic glycosylation in aqueous solution. The designed based on use thermosensitive polymer peptide synthesizer. This study represents proof‐of‐concept demonstration can be...
Abnormal expression of sialylated Thomsen–Friedenreich antigen (Neu5Acα2-3Galβ1-3GalNAcα-O-Ser/Thr, sialyl-T) has a strong relationship with various types human cancers and many other diseases. However, the size structural complexity, relatively lower abundance sialyl-T have posed significant challenge to its detection. Therefore, details about role in variety physiological pathological processes are still poorly understood. Here, one-step chemoenzymatic labeling strategy probe is described....
Studies of rare ketoses have been hampered by a lack efficient preparation methods. A convenient, efficient, and cost-effective platform for the facile synthesis is described. This method enables difficult-to-access ketopentoses ketohexoses from common inexpensive starting materials with high yield purity without need tedious isomer separation step.
Sialic acids are typically linked α(2-3) or α(2-6) to the galactose that located at non-reducing terminal end of glycans, playing important but distinct roles in a variety biological and pathological processes. However, details about their respective still largely unknown due lack an effective analytical technique. Herein, two-step chemoenzymatic approach for rapid sensitive detection N-acetylneuraminic acid-α(2-3)-galactose glycans is described.
Sugar nucleotides are essential glycosylation donors in the carbohydrate metabolism. Naturally, most sugar derived from a limited number of common by de novo biosynthetic pathways, undergoing single or multiple reactions such as dehydration, epimerization, isomerization, oxidation, reduction, amination, and acetylation reactions. However, it is widely believed that complex bioconversions not practical for synthetic use due to high preparation cost great difficulties product isolation....
Glycan is a crucial class of biological macromolecules with important functions. Functional groups determine the chemical properties glycans, which further affect their activities. However, structural complexity glycans has set technical hurdle for direct identification. Nanopores have emerged as highly sensitive biosensors that are capable detecting and characterizing various analytes. Here, we identified functional on designed α-hemolysin nanopore containing arginine mutations (M113R),...
Widely distributed in nature, sulfated glycan epitopes play important roles diverse pathophysiological processes. However, due to their structural complexity, the preparation of with structurally defined sulfation patterns is challenging, which significantly hampers detailed elucidation biological functions at molecular level. Here, we introduce a strategy for site-specific chemical epitopes, leveraging enzymatic sialylation and desialylation processes precisely control regio-specificity...
O-linked β-N-acetyl-glucosamine (O-GlcNAc) is an essential and ubiquitous post-translational modification present in nucleic cytoplasmic proteins of multicellular eukaryotes. The metabolic chemical probes such as GlcNAc or GalNAc analogues bearing ketone azide handles, conjunction with bioorthogonal reactions, provide a powerful approach for detecting identifying this modification. However, these either enter multiple glycosylation pathways have low labeling efficiency. Therefore, selective...
O-linked N-acetylglucosamine (O-GlcNAcylation) is a ubiquitous post-translational modification of proteins that essential for cell function. Perturbation O-GlcNAcylation leads to altered cell-cycle progression and DNA damage response. However, the underlying mechanisms are poorly understood. Here, we develop highly sensitive one-step enzymatic strategy capture profiling O-GlcNAcylated in cells. Using this strategy, discover flap endonuclease 1 (FEN1), an enzyme synthesis, novel substrate...
Core fucosylation and O-GlcNAcylation are the two most famous protein glycosylation modifications that regulate diverse physiological pathological processes in living organisms. Here, a "two birds one stone" strategy has been described for site-specific analysis of core O-GlcNAcylation. Taking advantage mutant endoglycosidases (EndoF3-D165A EndoCC-N180H), which efficiently specifically recognize fucose O-GlcNAc, glycopeptides can be labeled using biantennary N-glycan probe bearing azido...
Core fucosylation, a special type of N-linked glycosylation, is important in tumor proliferation, invasion, metastatic potential, and therapy resistance. However, the core-fucosylated glycoproteome has not been extensively profiled due to low abundance poor ionization efficiency glycosylated peptides. Here, "one-step" strategy described for protein core-fucosylation characterization biological samples. Core-fucosylated peptides can be selectively labeled with probe, which linked...
Neisseria meningitidis is a leading pathogen of epidemic bacterial meningitis and fulminant sepsis worldwide. Twelve different N. serogroups have been identified to date based on antigenic differences in the capsular polysaccharide. However, more than 90% human cases are result infection with just five serogroups, A, B, C, W135, Y. Efficient methods detection genogrouping isolates needed, therefore, order monitor prevalent as means disease control prevention. The gene complex regions...
Roles of internal epitopes and modification terminal in the glycan-binding events have been studied.
An efficient and convenient platform for the facile synthesis of phosphorylated ketoses is described. All eight ketopentoses were produced using this starting from two common inexpensive aldoses (d-xylose l-arabinose) in more than 84% isolated yield (gram scale). In method, reversible conversions (isomerization or epimerization) accurately controlled toward formation desired ketose phosphates by targeted phosphorylation reactions catalyzed substrate-specific kinases. The byproducts...
In recent years, the development of enzymatic bioorthogonal labeling strategy has offered exciting possibilities in probing structure-defined glycan epitopes. This takes advantage relaxed donor specificity and strict acceptor glycosyltransferases to label target epitopes with reactive groups carried by unnatural nucleotide sugars vitro. The subsequent covalent conjugation chemical reactions either fluorescent or affinity tags allows further visualization, quantification, enrichment However,...