A5090832501- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Hepatitis C virus research
- Carcinogens and Genotoxicity Assessment
- DNA and Nucleic Acid Chemistry
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Natural Language Processing Techniques
- Cytokine Signaling Pathways and Interactions
- Topic Modeling
- Bacteriophages and microbial interactions
- HIV/AIDS Research and Interventions
- Force Microscopy Techniques and Applications
- Histone Deacetylase Inhibitors Research
- Bacterial Genetics and Biotechnology
- Cancer-related gene regulation
- Hepatitis B Virus Studies
- Mosquito-borne diseases and control
- Melanoma and MAPK Pathways
- Ginseng Biological Effects and Applications
- Trace Elements in Health
Affiliated Hospital of Southwest Medical University
2020-2025
Leibniz Institute of Plant Genetics and Crop Plant Research
2025
Southwest Medical University
2022-2024
First Affiliated Hospital of Guangzhou University of Chinese Medicine
2020-2023
Chinese Research Academy of Environmental Sciences
2023
GlaxoSmithKline (United States)
2011-2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2021-2022
Emory University
2013-2021
Children's Healthcare of Atlanta
2021
Alibaba Group (China)
2021
The base 5-hydroxymethylcytosine (5hmC) was recently identified as an oxidation product of 5-methylcytosine in mammalian DNA. Here, using sensitive and quantitative methods to assess levels 5-hydroxymethyl-2'-deoxycytidine (5hmdC) 5-methyl-2'-deoxycytidine (5mdC) genomic DNA, we investigated whether 5hmC can distinguish normal tissue from tumor tissue. In squamous cell lung cancers, 5hmdC were depleted substantially with up 5-fold reduction compared brain tumors, showed even more drastic...
DNA methylation in mammals is highly dynamic during germ cell and preimplantation development but relatively static the of somatic tissues. 5-hydroxymethylcytosine (5hmC), created by oxidation 5-methylcytosine (5mC) Tet proteins most abundant brain, thought to be an intermediary toward 5mC demethylation. We investigated patterns 5hmC neurogenesis embryonic mouse brain. levels increase neuronal differentiation. In cells, not enriched at enhancers associates preferentially with gene bodies...
DinB, a Y-family DNA polymerase, is conserved among all domains of life; however, its endogenous substrates have not been identified. DinB known to synthesize accurately across number N 2 -dG lesions. Methylglyoxal (MG) common byproduct the ubiquitous glycolysis pathway and induces formation -(1-carboxyethyl)-2′-deoxyguanosine ( -CEdG) as major stable adduct. Here, we found that -CEdG could be detected at frequency one lesion per 10 7 nucleosides in WM-266-4 human melanoma cells, treatment...
ABSTRACT Chikungunya virus (CHIKV) represents a reemerging global threat to human health. Recent outbreaks across Asia, Europe, Africa, and the Caribbean have prompted renewed scientific interest in this mosquito-borne alphavirus. There are currently no vaccines against CHIKV, treatment has been limited nonspecific antiviral agents, with suboptimal outcomes. Herein, we identified β- d - N 4 -hydroxycytidine (NHC) as novel inhibitor of CHIKV. NHC behaves pyrimidine ribonucleoside selectively...
Chrysin (CH), a natural plant flavonoid, has shown variety of beneficial effects. Our present study was conducted to evaluate the therapeutic potential CH three days after spinal cord injury (SCI) in rats and probe underlying neuroprotective mechanisms. SCI induced using modified weight-drop method Wistar rats. Then, they were treated with saline or by doses 30 100 mg/kg for 26 days. Neuronal function assessed Basso Beattle Bresnahan locomotor rating scale (BBB). The water content determined...
Since HIV-associated neurocognitive disorders (HANDs) occur in up to half of HIV-positive individuals, even with combined antiretroviral therapy (cART), adjunctive therapies are needed. Chronic CNS inflammation contributes HAND and HIV encephalitis (HIVE). Baricitinib is a JAK 1/2 inhibitor approved the USA, EU, Japan for rheumatoid arthritis, demonstrating potent inhibition IL-6, D-dimer, CRP, TNF-α, IFN-α/β, other pro-inflammatory cytokines.Our modified murine model was used evaluate...
Abstract Micro/nanoscale 3D bioelectrodes gain increasing interest for electrophysiological recording of electroactive cells. Although printing has shown promise to flexibly fabricate bioelectronics compared with conventional microfabrication, relatively‐low resolution limits the printed bioelectrode high‐quality signal monitoring. Here, a novel multi‐material electrohydrodynamic (EHDP) strategy is proposed sub‐microscale gold pillars in vitro recordings. EHDP employed conductive circuits...
Understanding the role of heterotic genes in contributing to heterosis is essential for advancing hybrid breeding. We analyzed plant height (PH), ear (EH), and transcriptomic data from a maize population. GWAS identified that dominance effects QTL play significant traits mid-parent heterosis. By integrating GWAS, expression (eGWAS) analysis, module eGWAS, six candidate underlying were prioritized, including one spanned bZIP29 gene. In population, exhibited additive both heterosis, with its...
G[8−5m]T, a guanine−thymine intrastrand cross-link lesion where the C8 of guanine is covalently bonded to neighboring 3'-thymine through its methyl carbon, was previously shown form in an aqueous solution duplex DNA upon exposure γ- or X-rays and calf thymus treated with Fenton reagents. Here, we employed LC−MS/MS demonstrated for first time that this could be induced dose-dependent manner human Hela-S3 cells γ-rays. We further carried out vitro replication studies on substrate containing...
To counteract the deleterious effects of DNA damage, cells are equipped with specialized polymerases to bypass lesions. Previous biochemical studies revealed that DinB family polymerases, including Escherichia coli polymerase IV and human κ, efficiently incorporate correct nucleotide opposite some N2-modified 2′-deoxyguanosine derivatives. Herein, we used shuttle vector technology demonstrated deficiency in Polk or Poli mouse embryonic fibroblast (MEF) resulted elevated frequencies G→T G→A...
ABSTRACT The integrase inhibitors elvitegravir (EVG) and dolutegravir (DTG) rapidly decrease the plasma HIV-1 viral load, a key factor in prevention of maternal-to-fetal transmission HIV-1. No data have been reported on concentrations these drugs cord blood, maternal peripheral blood mononuclear cells (PBMCs), or placental tissue pregnant women. We present vivo pharmacokinetic antiretrovirals (ARV) within placentae from HIV-1-infected Maternal were obtained women receiving EVG, cobicistat,...
Reactive oxygen species can induce the formation of not only single-nucleobase lesions, which have been extensively studied, but also tandem lesions. Herein, we report a high frequency type lesion, where two commonly observed oxidatively induced that is, thymidine glycol (Tg) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), are vicinal to each other in calf thymus DNA upon exposure Cu(II)/ascorbate along with H2O2 or γ-rays. We further explored how lesions perturb efficiency fidelity...
Environmental chemicals and radiation have often been implicated in producing alterations of the epigenome thus potentially contributing to cancer other diseases. Ionizing radiation, released during accidents at nuclear power plants or after atomic bomb explosions, is a serious health threat for exposed human population. This type high-energy causes DNA damage including single- double-strand breaks induces chromosomal rearrangements mutations, but it not known if ionizing directly changes...
There are many reports that dietary supplementation with plant polysaccharides in pigs might promote their growth, but little is known about the maternal effect of ginseng (GPS) on piglets' growth by to pregnant and lactating sows. In current study, effects GPS immunity sows piglets were investigated. Results showed no significant difference among four groups total number piglets, live weak birth weight indicating GPS-treatment has adverse reproduction. Furthermore, weaning GPS-treated was...
Abstract Background Proteinase-activated-receptor-2 (PAR 2 ) is a seven transmembrane receptor that can activate two separate signaling arms: one through Gαq and Ca 2+ mobilization, second recruitment of β-arrestin scaffolds. In some cases downstream targets the Gαq/Ca arm are directly inhibited by β-arrestins, while in other pathways synergistic; thus β-arrestins act as molecular switches capable modifying signal generated receptor. Results Here we demonstrate PAR adenosine...
Diversity-generating retroelements (DGRs) create unparalleled levels of protein sequence variation through mutagenic retrohoming. Sequence information is transferred from an invariant template region (TR), RNA intermediate, to a protein-coding variable region. Selective infidelity at adenines during transfer hallmark DGRs disparate bacteria, archaea, and microbial viruses. We recapitulated selective in vitro for the prototypical Bordetella bacteriophage DGR. A complex DGR reverse...
Chronic liver inflammation precedes the majority of hepatocellular carcinomas (HCC). Here, we explore connection between chronic and DNA methylation in at late precancerous stages HCC development Mdr2(-/-) (Mdr2/Abcb4-knockout) mice, a model inflammation-mediated HCC. Using methylated immunoprecipitation followed by hybridization with "CpG islands" (CGIs) microarrays, found specific CGIs 76 genes which were hypermethylated compared to age-matched healthy controls. The observed...