A5100320073- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Plant Genetic and Mutation Studies
- Peanut Plant Research Studies
- Plant Stress Responses and Tolerance
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Coconut Research and Applications
- Plant Gene Expression Analysis
- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Insect Resistance and Genetics
- Pluripotent Stem Cells Research
- Renal and related cancers
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Imbalanced Data Classification Techniques
- Biocrusts and Microbial Ecology
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- RNA Interference and Gene Delivery
- Financial Distress and Bankruptcy Prediction
- Epigenetics and DNA Methylation
- Insect symbiosis and bacterial influences
Qingdao Agricultural University
2022-2024
Southwest University
2014-2024
University of Michigan
2021-2024
Xinqiao Hospital
2023-2024
Army Medical University
2023-2024
Chinese Academy of Agricultural Sciences
2020-2024
Ministry of Agriculture and Rural Affairs
2021-2024
Southern University of Science and Technology
2019-2024
Oil Crops Research Institute
2020-2024
China Pharmaceutical University
2023
Flowering plants sense various environmental and endogenous signals to trigger the floral transition start reproductive growth cycle. CONSTANS (CO) is a master transcription factor in photoperiod pathway that integrates upstream activates florigen gene FLOWERING LOCUS T (FT). Here, we performed comprehensive structural biochemical analyses study molecular mechanism underlying regulation of FT by CO Arabidopsis thaliana. We show four previously characterized cis-elements promoter proximal...
SMALL AUXIN UP-REGULATED RNAs (SAURs) are recognized as auxin-responsive genes involved in the regulation of abiotic stress adaptive growth. Among growth-limiting factors, water-deficit condition significantly affects plant growth and development. The putative function SAUR family member AtSAUR32 has potential to diminish negative impact drought stress, but exact mode action remain unclear Arabidopsis. In current study, gene was cloned functionally analyzed. localized plasma membrane nucleus...
Abstract Pancreatic β-cell compensation is a major mechanism in delaying T2DM progression. Here we report the abnormal high expression of circGlis3 islets male mice with obesity and serum people obesity. Increasing regulated by Quaking (QKI)-mediated splicing circularization. overexpression enhances insulin secretion inhibits obesity-induced apoptosis vitro vivo. Mechanistically, promotes up-regulating NeuroD1 Creb1 via sponging miR-124-3p decreases interacting pro-apoptotic factor SCOTIN....
Missense mutations in the p53 DNA-binding domain (DBD) contribute to half of new cancer cases annually. Here we present a thermodynamic model that quantifies and links major pathways by which inactivate p53. We find DBD possesses two unusual properties—one highest zinc affinities any eukaryotic protein extreme instability absence zinc—which are predicted poise on cusp folding/unfolding cell, with determinant being available concentration. analyze 20 most common tumorigenic 80% impair...
DNA binding with one finger (Dof) transcription factors play important roles in plant growth and abiotic stress responses. Although genome-wide identification analysis of the DOF factor family has been reported other species, no relevant studies have emerged wheat. The aim this study was to investigate evolutionary functional characteristics associated responses by wheat Dof gene family.Using recently released genome database (IWGSC RefSeq v1.0), we identified 96 members, which were...
The Ethylene-responsive element binding factor-associated Amphiphilic Repression (EAR) motifs, which were initially identified in members of the Arabidopsis ethylene response factor (ERF) family, are transcriptional repression motifs plants and defined by consensus sequence patterns either LxLxL or DLNxxP. EAR motif-containing proteins can function as transcription repressors, thus interacting with co-repressors, such TOPLESS AtSAP18, affecting structure chromatin histone modifications...
Spraying N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU), an exogenous cytokinin (CK) growth regulator, is the conventional method for inducing fruit set during melon (Cucumis melo L.) production; however, mechanism by which CPPU induces unclear. Through histological and morphological observations, size was comparable between CPPU-induced fruits normal pollinated because had higher cell density but smaller compared with fruits. promotes accumulation of gibberellin (GA) auxin decreases level...
Grafting is an important approach to improving tea plant varieties, and grafting can result in changes secondary metabolites. However, the shifting pattern of metabolites between scions, rootstocks, non-grafted leaves unclear. We employed “Yuncha 1” as scion “Zijuan” rootstock with significant differences leaf color traits reveal influences on generation transcriptional activities. By non-targeted metabolomic analysis, we identified that led obvious shift scion, rootstock, leaves....
The auxin response factor (ARF) gene family is involved in plant development and hormone regulation. Although the ARF has been studied some species, its structural features, molecular evolution, expression profiling Brachypodium distachyon L. are still not clear.Genome-wide analysis identified 19 genes B. distachyon. A phylogenetic tree constructed with 182 from seven species revealed three different clades, within a clade exhibited conservation, although certain divergences occurred clades....
High-throughput genetic screens are powerful methods to interrogate gene function on a genome-wide scale and identify genes responsible certain stresses. Here, we developed piggyBac strategy deliver pooled sgRNA libraries stably into cell lines. We used this conduct screen based clustered regularly interspaced short palindromic repeat technology (CRISPR)-Cas9 in Bombyx mori cells. first constructed single guide RNA (sgRNA) library containing 94,000 sgRNAs, which targeted 16,571...
Abstract Background Coat color determines both appearance and nutrient quality of peanut. White seed coat in peanut can enhance the processing efficiency oil. An integrative analysis transcriptomes, metabolomes histocytology was performed on wsc mutant its wild type to investigate regulatory mechanisms underlying pigmentation. Result Metabolomes revealed flavonoids were redirected , while multi-omics analyses seeds testae uncovered WSC influenced biosynthesis testa as well suberin formation,...
Endogenous retroviruses (ERVs) have been reported to participate in pre-implantation development of mammalian embryos. In early human embryogenesis, different ERV sub-families are activated a highly stage-specific manner. How the specificity activation is achieved remains largely unknown. Here, we demonstrate mechanism how LTR7Ys, morula-blastocyst-specific HERVH long terminal repeats, by naive pluripotency transcription network. We find that KLF5 interacts with and rewires NANOG bind...
As one of the most important transcription factor families, GRAS proteins are involved in numerous regulatory processes, especially plant growth and development. However, they have not been systematically analyzed Brachypodium distachyon, a new model grass. In this study, 48 BdGRAS genes were identified. Duplicated account for 41.7% them contribute to expansion gene family. 33, 39, 35 identified by synteny with their orthologs rice, sorghum, maize wheat genome, respectively, indicating close...
Large-scale genetic mutant libraries are powerful approaches to interrogating genotype–phenotype correlations and identifying genes responsible for certain environmental stimuli, both of which the central goal life science study. We produced first large-scale CRISPR-Cas9-induced library in a nonmodel multicellular organism, Bombyx mori . developed piggyBac -delivered binary genome editing strategy, can simultaneously meet requirements mixed microinjection, efficient multipurpose operation,...
Introduction Peanut ( Arachis hypogaea L.), also called groundnut is an important oil and cash crop grown widely in the world. The annual global production of groundnuts has increased to approximately 50 million tons, which provides a rich source vegetable oils proteins for humans. Low temperature (non-freezing) one major factors restricting peanut growth, yield, geographic distribution. Since complexity cold-resistance trait, molecular mechanism cold tolerance related gene networks were...
Abstract DNA methylation in the non-CG context is widespread plant kingdom and abundant mammalian tissues such as brain pluripotent cells. Non-CG Arabidopsis thaliana coordinately regulated by DOMAINS REARRANGED METHYLTRANSFERASE (DRM) CHROMOMETHYLASE (CMT) proteins but has yet to be systematically studied major crops due difficulties obtaining genetic materials. Here, utilizing highly efficient multiplex CRISPR-Cas9 genome-editing system, we created single- multiple-knockout mutants for all...
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Plant growth and development process is indeed the cell meristematic process, fate transition accompanied by more obvious changes in epigenetic modification. Callus with pluripotency, has ability to continue division, ideal for studying plant differentiation. By comparing different modifications between callus seedling, of chromatin state, effects on plants can be revealed, key genes related uncovered, thus know regulation development. In this study, we performed ChIP assays antibodies...