Abstract The CRISPR/Cas9 system has successfully been used in various organisms for precise targeted gene editing. Although it demonstrated that can induce mutation tomato plants, the stability of heredity later generations and mutant specificity induced by plants have not yet elucidated detail. In this study, two genes, SlPDS SlPIF4 , were testing mutagenesis through an Agrobacterium tumefaciens -mediated transformation method. A high frequency was observed all tested targets T0 transgenic...

Mitogen-activated protein kinase (MAPK) cascades have important functions in plant growth, development, and response to various stresses. The MAPKK MAPKKK gene families tomato never been systematically analyzed. In this study, we performed a genome-wide analysis of the identified 5 genes 89 genes. Phylogenetic analyses showed that all formed four groups (groups A, B, C, D), whereas were classified into three subfamilies, namely, MEKK, RAF, ZIK. Evolutionary whole genome or chromosomal...

SMALL AUXIN UP-REGULATED RNAs (SAURs) are recognized as auxin-responsive genes involved in the regulation of abiotic stress adaptive growth. Among growth-limiting factors, water-deficit condition significantly affects plant growth and development. The putative function SAUR family member AtSAUR32 has potential to diminish negative impact drought stress, but exact mode action remain unclear Arabidopsis. In current study, gene was cloned functionally analyzed. localized plasma membrane nucleus...

Watermelon (Citrullus lanatus L.), which is an economically important cucurbit crop that cultivated worldwide, vulnerable to various adverse environmental conditions. Small heat shock protein 20s (HSP20s) are the most abundant plant HSPs and they play roles in biotic abiotic stress responses. However, have not been systematically investigated watermelon. In this study, we identified 44 watermelon HSP20 genes analyzed their gene structures, conserved domains, phylogenetic relationships,...

The two-component system (TCS), which comprises histidine kinases (HKs), phosphotransfers (HPs), and response regulator proteins (RRs), plays pivotal roles in regulating plant growth, development, responses to biotic abiotic stresses. TCS genes have been comprehensively identified investigated various crops but poorly characterized tomato. In this work, a total of 65 consisting 20 HK(L)s, six HPs, 39 RRs were from tomato genome. classification, gene structures, conserved domains, chromosome...

Mitogen-activated protein kinases (MAPKs) regulate diverse aspects of plant growth. However, their potential role in reproductive development remains elusive. Here, we discovered an unique SlMPK20, a plant-specific group D MAPK, pollen tomato. RNAi-mediated suppression SlMPK20 or its knockout using CRISPR/Cas9 significantly reduced completely abolished viability, respectively, with no effects on maternal fertility. Cell biology and gene expression analyses established that exerts...

Cucumber green mottle mosaic virus (CGMMV) causes substantial global losses in cucurbit crops, especially watermelon. N6-methyladenosine (m6A) methylation RNA is one of the most important post-transcriptional modification mechanisms eukaryotes. It has been shown to have regulatory functions some model plants, but there no research regarding m6A modifications watermelon.We measured level resistant watermelon after CGMMV infection using a colorimetric method. And results found that...

Abstract Plant height is a key agronomic trait regulated by several phytohormones such as gibberellins (GAs) and auxin. However, little known about how cytokinin (CK) participates in this process. Here, we report that SlRR6, type-A response regulator the CK signaling pathway, positively regulates plant tomato. SlRR6 was induced exogenous kinetin GA3, but inhibited indole-3-acetic acid (IAA). Knock out of reduced tomato through shortening internode length, while overexpression caused taller...

SMALL AUXIN UP-REGULATED RNA (SAURs) genes are acknowledged as auxin-responsive that play crucial roles in modulating adaptive growth under abiotic stress conditions. Low temperatures constitute a primary limiting factor significantly impairs the development, growth, and fruit quality of watermelon plants during winter spring seasons. Despite their potential importance, SAURs have not yet been thoroughly investigated or characterized watermelon. In this study, we identified positive...

Valine-glutamine (VQ) motif-containing proteins play important roles in diverse plant developmental processes and signal transduction response to biotic abiotic stresses. However, no systematic investigation has been conducted on VQ genes watermelon. In this study, we identified 31 watermelon genes, which were classified into six subfamilies (I–VI). All of the deduced contained a conserved FxxxVQxL/F/VTG motif. Eleven ClVQs involved segment duplication, was main factor expansion family...

DNA methylation is an important epigenetic mark associated with plant immunity, but little known about its roles in viral infection of watermelon. We carried out whole-genome bisulfite sequencing watermelon leaves at 0 h (ck), 48 h, and 25 days post-inoculation Cucumber green mottle mosaic virus (CGMMV). The number differentially methylated regions (DMRs) increased during CGMMV 2788 DMR-associated genes (DMGs) were screened among three libraries. Most DMRs DMGs obtained under the CHH...

Abstract Background Auxin signaling has a vital function in the regulation of plant growth and development, both which are known to be mediated by auxin-responsive genes. So far, significant progress been made toward identification characterization auxin-response genes several model plants, while no systematic analysis for these families was reported cucumber ( Cucumis sativus L.), reference species Cucurbitaceae crops. The comprehensive analyses will help design experiments functional...

MicroRNAs (miRNAs), a class of non-coding small RNAs (sRNAs), regulate various biological processes. Although miRNAs have been identified and characterized in several plant species, Asparagus officinalis not reported. As dioecious with homomorphic sex chromosomes, asparagus is regarded as an important model system for studying mechanisms determination. Two independent sRNA libraries from male female plants were sequenced Illumina sequencing, thereby generating 4.13 5.88 million final clean...

Cucumber green mottle mosaic virus (CGMMV) is a member of the genus Tobamovirus, which cause diseases in cucurbits, especially watermelon. In watermelon, symptoms develop on whole plant, including leaves, stems, peduncles, and fruit. To better understand molecular mechanisms watermelon early responses to CGMMV infection, comparative transcriptome analysis 24 h CGMMV-infected mock-inoculated leaves was performed. A total 1641 differently expressed genes (DEGs) were identified, with 886 DEGs...

Cucumber and watermelon, which belong to Cucurbitaceae family, are economically important cultivated crops worldwide. However, these vulnerable various adverse environments. Two-component system (TCS), consisting of histidine kinases (HKs), phosphotransfers (HPs), response regulator proteins (RRs), plays roles in plant developmental processes signalling transduction responses a wide range biotic abiotic stresses. No systematic investigation has been conducted on TCS genes species. Based the...

Abstract Dwarf architecture is an important trait associated with plant yield, lodging resistance and labor cost. Here, we aimed to identify a gene causing dwarfism in watermelon. The ‘w106’ (dwarf) ‘Charleston Gray’ (vine) were used as parents construct F 1 2 progeny. of was mainly caused by longitudinal cell length reduction controlled single recessive gene. Whole-genome sequencing two bulk DNAs population localized this 2.63-Mb region on chromosome 9; further narrowed 541-kb region....

Heat-shock protein 20s (HSP20) were initially shown to play a role during heat shock stress; however, recent data indicated that HSP20 proteins are also involved in abiotic stress plants. Watermelon is known be vulnerable various stressors; have yet investigated and characterized the watermelon. In previous study, we identified negative regulator of salt response from watermelon: ClHSP22.8 , member family. Quantitative real-time PCR (qRT-PCR) promoter::β-glucuronidase ( GUS ) analysis...

Background CDGSH iron-sulfur domain 2 (CISD2), an protein with a [2Fe-2S] cluster, plays pivotal role in the progression of various cancers, including Diffuse Large B-cell Lymphoma (DLBCL). However, mechanisms by which CISD2 regulates occurrence and development DLBCL remain to be fully elucidated. Methods The potential as predictive marker patients treated R-CHOP regimen was investigated through bioinformatics analysis clinical cohort studies. cell lines (SUDHL-4 HBL-1) were employed this...

Increased consumption of vegetables has been recommended worldwide as a part healthy diet; therefore, determining gene function among breeding materials is crucial for vegetable improvement to meet the sustainable development new varieties. However, genetic transformation time-consuming and laborious, which limits exploration various crops. Virus-Induced Gene Silencing (VIGS) can perform large-scale rapid silencing in plants due reduction experimental period its independence from stable...

Mantle cell lymphoma (MCL) is a rare and aggressive form of non-Hodgkin lymphoma. Challenges in its treatment include relapse, drug resistance, short survival period. The Hedgehog/GLI1 (Hh/GLI1) Wnt/β-catenin pathways are crucial cancer proliferation, survival, making them significant targets for anticancer research. This study aimed to assess the effectiveness combining inhibitors both against MCL investigate underlying molecular mechanisms. co-expression key proteins from Hh/GLI1 was...