A5088259187- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- 3D Printing in Biomedical Research
- Neurological Disorders and Treatments
- CAR-T cell therapy research
- SARS-CoV-2 and COVID-19 Research
- Renal and related cancers
- Liver physiology and pathology
- Animal Virus Infections Studies
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Biomedical Ethics and Regulation
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Reproductive Biology and Fertility
- Virus-based gene therapy research
- HIV Research and Treatment
- Natural Antidiabetic Agents Studies
- Animal Genetics and Reproduction
- Congenital heart defects research
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Hematopoietic Stem Cell Transplantation
Peking University
2016-2025
Center for Life Sciences
2016-2025
Beijing National Laboratory for Molecular Sciences
2024-2025
Sichuan University
2025
State Key Laboratory of Biotherapy
2025
Qingdao University
2024
Beijing HuiLongGuan Hospital
2024
Shanghai University of Traditional Chinese Medicine
2024
State Key Laboratory of Natural and Biomimetic Drugs
2015-2023
Shenyang Jianzhu University
2022
Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation technically challenging strategies such as nuclear transfer used reprogramming limit their clinical applications. Here, we show that pluripotent generated mouse at a frequency up to 0.2% using combination of seven small-molecule compounds. The chemically resemble embryonic terms gene expression...
The differentiation capacity of human embryonic stem cells (hESCs) holds great promise for therapeutic applications. We report a novel three-stage method to efficiently direct the into hepatic in serum-free medium. Human ESCs were first differentiated definitive endoderm by 3 days Activin A treatment. Next, presence fibroblast growth factor-4 and bone morphogenetic protein-2 culture medium 5 induced efficient from cells. Approximately 70% expressed marker albumin. After 10 further vitro...
The biological phenotype of primary human immunodeficiency virus type 1 (HIV-1) isolates varies according to the severity HIV infection. Here we show that two previously described groups rapid/high, syncytium-inducing (SI) and slow/low, non-syncytium-inducing (NSI) are distinguished by their ability utilize different chemokine receptors for entry into target cells. Recent studies have identified C-X-C receptor CXCR4 (also named fusin or Lestr) C-C CCR5 as principal cofactors...
Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here screening Chinese herbal medicine-based, novel small molecules that bind avidly with surface spike protein SARS-CoV and thus can interfere entry virus to its host cells. achieved this by using two-step method consisting frontal affinity chromatography-mass spectrometry coupled viral infection assay based on human immunodeficiency (HIV)-luc/SARS pseudotyped...
Bats may host emerging viruses, including coronaviruses (CoV). We conducted an evaluation of CoV in rhinolophid and vespertilionid bat species common Europe. Rhinolophids carried severe acute respiratory syndrome (SARS)-related at high frequencies concentrations (26% animals are positive; up to 2.4×10(8) copies per gram feces), as well two Alphacoronavirus clades, one novel related the HKU2 clade. All three clades present Miniopterus bats China (HKU7, HKU8, 1A related) were also European...
The safety of CRISPR (clustered regularly interspaced short palindromic repeats)-based genome editing in the context human gene therapy is largely unknown. CCR5 a reasonable but not absolutely protective target for cure immunodeficiency virus type 1 (HIV-1) infection, because CCR5-null blood cells are resistant to HIV-1 entry. We transplanted CRISPR-edited CCR5-ablated hematopoietic stem and progenitor (HSPCs) into patient with infection acute lymphoblastic leukemia. leukemia was complete...
Abstract Cellular senescence, a persistent state of cell cycle arrest, accumulates in aged organisms, contributes to tissue dysfunction, and drives age-related phenotypes. The clearance senescent cells is expected decrease chronic, low-grade inflammation improve repair capacity, thus attenuating the decline physical function organisms. However, selective effective different types has proven challenging. Herein, we developed prodrug strategy design new compound based on increased activity...