A5086790890- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Advanced Nanomaterials in Catalysis
- Advanced Memory and Neural Computing
- Pancreatic function and diabetes
- Shape Memory Alloy Transformations
- Cancer Cells and Metastasis
- Metal-Organic Frameworks: Synthesis and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Transition Metal Oxide Nanomaterials
- Graphene and Nanomaterials Applications
- Nanocluster Synthesis and Applications
- Nanoplatforms for cancer theranostics
- Diabetes and associated disorders
- Congenital heart defects research
- Hippo pathway signaling and YAP/TAZ
Peking University
2013-2025
State Key Laboratory of Natural and Biomimetic Drugs
2017-2024
Center for Life Sciences
2013-2023
University of California, Berkeley
2013
Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation technically challenging strategies such as nuclear transfer used reprogramming limit their clinical applications. Here, we show that pluripotent generated mouse at a frequency up to 0.2% using combination of seven small-molecule compounds. The chemically resemble embryonic terms gene expression...
Abstract The capacity of 3D organoids to mimic physiological tissue organization and functionality has provided an invaluable tool model development disease in vitro. However, conventional organoid cultures primarily represent the homeostasis self-organizing stem cells their derivatives. Here, we established a novel intestinal culture system composed 8 components, mainly including VPA, EPZ6438, LDN193189, R-Spondin 1 conditioned medium, which mimics gut epithelium regeneration that produces...
Miniaturization of the steam engine to microscale is hampered by severe technical challenges. Microscale mechanical motion typically actuated with other mechanisms ranging from electrostatic interaction, thermal expansion, and piezoelectricity more exotic types including shape memory, electrochemical reaction, responsivity polymers. These offer either large-amplitude or high-speed actuation, but not both. In this work we demonstrate working principle a solid (μSE) based on phase transition...
Bimetallic coordination polymers (CPs) have two different metal ions as connecting nodes in their polymer structure. The synthesis methods of bimetallic CPs are mainly categorized into the one-pot method and post-synthesis modifications according to various needs. Compared with monometallic CPs, synergistic effects excellent properties, such higher gas adsorption rate, more efficient catalytic stronger luminescent stable loading platforms, which been widely applied fields adsorption,...
There is a widely accepted material characterization paradigm in the success of synthesis luminescent metal nanoclusters (NCs) aqueous phase: new emission, reduction, and ultrasmall particles (size < 3 nm). Herein, we falsified well-known fluorescent histidine (His)-directed Au NCs model metastable His–Au(I) complexes with emissive His oxidation products has been established. The redox reaction Au(III) yields oligomers blue-green fluorescence reducible Au(I) self-assemblies, which can form...
Human somatic cells can be reprogrammed to pluripotent stem by small molecules through an intermediate stage with a regeneration signature, but how this state is induced remains largely unknown. Here, integrated single-cell analysis of transcriptome, we demonstrate that the pathway human chemical reprogramming distinct from transcription-factor-mediated reprogramming. Time-course construction chromatin landscapes unveils hierarchical histone modification remodeling underlying program, which...
Abstract Pluripotent stem cells can be induced from somatic by the cellular intrinsic factors, including oocyte components or transcription factors 1-4 . Previously, we have demonstrated that mouse pluripotent small molecule-based external stimulation, providing a fundamentally different approach for inducing pluripotency 5-8 Here human molecules cells. This protocol describes detailed procedures induction of chemically (hCiPSCs) In this method, freshly isolated embryonic fibroblasts (HEFs),...