A5100324106- Hepatitis Viruses Studies and Epidemiology
- Liver Disease and Transplantation
- Viral gastroenteritis research and epidemiology
- Autophagy in Disease and Therapy
- Hepatitis C virus research
- Macrophage Migration Inhibitory Factor
- Cancer-related molecular mechanisms research
- NF-κB Signaling Pathways
- Employment and Welfare Studies
- Global Health Workforce Issues
- Viral Infections and Immunology Research
- Veterinary medicine and infectious diseases
- Hepatitis B Virus Studies
- Reproductive System and Pregnancy
- Diabetes and associated disorders
- Liver Diseases and Immunity
- Pancreatic function and diabetes
- Virology and Viral Diseases
- Cell death mechanisms and regulation
- Mesenchymal stem cell research
- Hepatocellular Carcinoma Treatment and Prognosis
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolism, Diabetes, and Cancer
- Cardiac, Anesthesia and Surgical Outcomes
- Cytomegalovirus and herpesvirus research
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2024
Institute of Medical Biology
2014-2024
Shandong Provincial Hospital
2020-2021
Shandong First Medical University
2020-2021
Shandong University
2020
Kunming University of Science and Technology
2011-2016
Institut de Recherche Vaccinale
2014
Hepatitis E virus (HEV) represents the main cause of acute hepatitis worldwide. HEV infection in immunocompromised patients involves a high risk for development chronic hepatitis. Because is recognized as zoonotic pathogen, it currently believed that swine primary reservoir. However, this not sufficient to justify strikingly seroprevalence both developing and Western countries. Thus, study aimed identify new sources bear transmission humans. We collected fecal, blood, milk samples cows...
Hepatitis E virus (HEV) is a major cause of acute hepatitis worldwide, primarily transmitted by fecal-oral route. Zoonotic transmission HEV from HEV-infected pigs (pork) or cows (milk) to human non-human primate has been confirmed, but the risk in goat still rarely assessed. In present study, stool, blood, tissues, and milk were collected for infection investigation Dali City Yunnan Province China, where raw mutton are traditionally consumed. Surprisingly, high prevalence was found....
Summary Hepatitis E virus ( HEV ) infection causes subclinical diseases, leading to high mortality (>25%) in pregnant women. replication is aggressively escalated women, especially the third trimester of pregnancy. Oestrogen plays an important role However, pathogenesis women or immunosuppressive (such as HIV ‐infected organ‐transplanted women) remains unclear. We investigated oestradiol a cell culture system. had significantly higher levels compared with uninfected individuals. was...
Worldwide, the Hepatitis E virus (HEV) is main pathogen of acute viral hepatitis, with an extremely high mortality in pregnant women. However, pathogenesis HEV infection women remains largely unknown. We established HEV-infected mice animal model to explore adverse pregnancy outcomes infection. Mice were infected their early, middle and late stages pregnancy. RNA was detected tissues (liver, spleen, kidney, colon, uterus placenta) mice. antigens also these Miscarriages (7/8, 87.5%) occurred...
Hepatitis E virus (HEV) infection causes high mortality in pregnant women. However, the pathogenic mechanisms of HEV women remain unknown. In this study, roles pregnancy serum were investigated using an efficient cell culture system. was exacerbated by supplementing with serum, especially theat third trimester pregnancy. Oestrogen receptors (ER-α and ER-β) activated cells supplemented significantly inhibited during infection. Type I IFN, IFN-β, showed delayed upregulation HEV-infected...
Rhesus macaque is an important animal model in biomedical research, especially human disease, developmental, translational, and pre-clinical research. Blood physiological biochemical parameters are markers for physiology, pathology, toxicology However, these have not been systematically reported Chinese rhesus macaques. To characterize the reference parameters, this study collected 1805 macaques living Southwestern China. A total of 24 blood indexes 27 were determined. Sex age found to...
Abstract Hepatitis E virus (HEV) is the major pathogen of viral hepatitis. HEV causes high mortality in pregnant women. Its infection during pregnancy usually leads to fulminant hepatic failure, spontaneous abortions, premature delivery, or stillbirth. Vertical transmission has been reported, but pathogenesis remains largely elusive. Pregnant rhesus macaques were infected with explore genotype 4 pregnancy. Active infections established shedding viruses feces and blood, elevated liver...
Hepatitis E virus (HEV) persists in the male genital tract that associates with infertility. However, presence of HEV female is unreported. Vaginal secretions, cervical smears, and cervix uteri were collected to explore tract. RNA and/or antigens detected vaginal women. The infectivity excreted into secretions was further validated vitro. In addition, replicates identified HEV-infected animal models by injection or mucosal infection imitate sexual transmission. Serious damage inflammatory...
Hepatitis E virus (HEV) is a major cause of hepatitis in developing countries and poses threat to public health worldwide. Tree shrew (Tupaia belangeri chinensis) useful animal model studies on viruses, such as B C viruses. However, the use this for HEV research yet be developed. shrews were intravenously (IV) injected with swine genotype 4 or infected by contact-exposure IV tree shrews. RT-nPCR was performed detect RNA feces, tissues, blood. capsid protein different tissues detected Western...
Following abdominal surgery, patients usually experience a transient episode of impaired gastrointestinal motility. This study aimed to determine whether single preoperative dose dexamethasone can promote the recovery function in following elective surgery.In this single-center, two-arm, parallel, randomized controlled trial, we studied 126 (aged 18-80 years) who underwent open or laparoscopic bowel surgery for malignant benign pathology. At induction anesthesia, treatment group (n = 64)...
: Background: Hepatitis E virus (HEV) infection is a significant public health concern and has been identified as zoonotic infection.Objectives: Since no reports have characterized the epidemiological genotypicfeatures of HEV infections in Macaca mulatta (rhesus macaques) from Yunnan, China, where swine are endemic, we aimed to investigate these characteristics.Materials Methods: Seroepidemiological molecular characterization both pigs Yunnan province China were conducted using enzyme-linked...
Hepatitis E virus (HEV) infection has become a global concern with high mortality rates among pregnant women, especially those in their third trimester of pregnancy. Estrogen plays an important role mediating the body, regulating physiological and pathological processes. is activated by binding to estrogen receptors (ERs) mediates rapid signaling events pathways that involve transmembrane ERs. Our previous study had confirmed levels during pregnancy are associated HEV titers. However,...
Hepatitis E virus (HEV) is the main pathogen of hepatitis worldwide. However, its infection biology and pathogenesis remain largely unknown. Suitable small animal models are required to advance study HEV infection. Although an efficient model genotype 1 (gt1) gt3 has been established in human liver chimeric mice, infectivity gt4 mice not comprehensively characterized. In this study, immunocompromised BALB/c nude, immunocompetent BALB/c, C57BL/6 were inoculated with either or (19 strains,...
To evaluate whether uterine injury caused by hepatitis E virus (HEV) infection is responsible for adverse pregnancy outcomes. HEV-infected female BALB/c mice were coupled with healthy male at 0, 7, 14, 21, and 91 dpi to explore the HEV infection. Mice euthanized after 10 days of copulation, uteruses collected RNA antigen detection histopathological analysis. Inflammatory responses; apoptosis; estrogen receptor ɑ (ER-ɑ), endomethal antibody (ERAb), cytokeratin-7 (CK7), vimentin (VIM),...
To build an ideal animal model for studying the mechanism of occurrence, developing and treating diabetes become a more important issue, facing with fact that big threat to human health has been worsen. First, we used normal control diets or high-fat/high-sucrose feed adult rhesus monkeys macaques induced by in group type 2 mellitus (T2DM) developed hyperglycemia, hyperinsulinemia at 6 months accordance precious researches reported minipigs, rats mice could develop hyperinsulinemia,...
Hepatitis E virus (HEV) is a leading cause of hepatitis worldwide. However, its infection biology and pathogenesis remain largely elusive. Furthermore, no proven medication available for treating E. Robust experimental models are urgently required to advance the research HEV infection. Because lacking sophisticated small animal model, this study aimed establish mouse model infection.We constructed full-length swine cDNA clone genotype 4 (named as pGEM-HEV) by reverse genetics approach. And...