A5053665396- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Chemical Synthesis and Analysis
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Amino Acid Enzymes and Metabolism
- Antimicrobial Peptides and Activities
- Genomics and Chromatin Dynamics
- Liver physiology and pathology
- Drug Transport and Resistance Mechanisms
- Pancreatic function and diabetes
- DNA Repair Mechanisms
- Protein Hydrolysis and Bioactive Peptides
- Click Chemistry and Applications
- Pesticide Residue Analysis and Safety
- Protein Interaction Studies and Fluorescence Analysis
- Pharmacological Effects and Toxicity Studies
- Analytical Chemistry and Chromatography
- Cancer-related Molecular Pathways
- HIV/AIDS drug development and treatment
- RNA modifications and cancer
- Analytical chemistry methods development
- Ubiquitin and proteasome pathways
Henan University of Technology
2015-2024
Shanghai Institute of Materia Medica
2024
Chinese Academy of Sciences
2024
University of Chinese Academy of Sciences
2024
Nanjing University of Chinese Medicine
2024
University of California, San Diego
2017
Peking University
2005-2012
Center for Life Sciences
2012
Henan University
2010
Henan University of Engineering
2009
The derivation of hepatic progenitor cells from human embryonic stem (hES) is value both in the study early liver organogenesis and creation an unlimited source donor for hepatocyte transplantation therapy. Here, we report first time generation derived hES cells. Hepatic endoderm were generated by activating FGF BMP pathways then purified fluorescence activated cell sorting using a newly identified surface marker, N-cadherin. After co-culture with STO feeder cells, these yielded colonies,...
Zinc finger proteins are associated with hereditary diseases and cancers. To obtain an adequate amount of zinc for studying their properties, structure, functions, many protein expression systems used. ZNF191(243-368) is a can be fused His-tag to generate fusion such as His6-ZNF191(243-368) ZNF191(243-368)-His8. The purification using Ni-NTA resin overcome the difficulty separation caused by inclusion body formation. influences on properties structure were investigated spectrographic...
Transcriptional machinery often operates in the configuration of biomolecular condensates, presenting emerging opportunities to develop therapies targeting transcriptionally addicted malignancies. In this study, we discovered that cyclin-dependent kinase 12 (CDK12), a key regulator transcription elongation, formed dynamic liquid-like droplets within nuclear speckles, process enhanced by high CDK12 expression and inhibitor treatment. Recognizing unique property, rationally designed ZSQ253,...
Abstract Background The usage of synonymous codons shows considerable variation among mammalian genes. How and why this is non-random are fundamental biological questions remain controversial. It also important to explore whether genes that selectively expressed at different developmental stages bear molecular features. Results In two models mouse stem cell differentiation, we established correlations between codon the patterns gene expression. We found optimal exhibited (AT- or GC-ending...
Objectives: To identify whether Nestin-positve cells or Nestin-negative in pancreas enrich potential pancreatic stem/progenitor cells. Methods: We generated transgenic mice carrying enhanced green fluorescent protein (EGFP) under the control of nestin second-intronic enhancer and subsequently divided their embryonic into different subpopulations according to expression EGFP CD24 characterized these by vitro culture. Results: The correlated well with that endogenous Nestin. Only...
Mutations in breast cancer susceptibility gene 2 (BRCA2) can lead to chromosomal instability and result cancer, which is strongly associated with p53 mutations. Here, based on the crystal structure of BRC4 p53, spatial BRC2 (171-192) was simulated, providing structural basis for site-specific mutation BRC2. The BRC analogous peptides were synthesized, interaction between mutant peptide studied using circular diachronic spectroscopy fluorescence spectroscopy. results show that mutations amino...
ZNF191(243-368) is the C-terminal region of ZNF191 which contains a putative DNA-binding domain four Cys 2 His zinc finger motifs. In this study, an expression vector fusion protein with glutathione-S-transferase (GST) was constructed and transformed into Escherichia coli BL21. The GST-ZNF191(243-368) expressed using to investigate protein-DNA binding reaction through affinity selection strategy on basis quality domain. Results showed that can selectively bind sequences react genes contain...
Ferulic acid was used as a common drug for cardia-cerebrovascular disease and leukopenia, but the application of ferulic inhibited by poor absorption stability. The improvement these defects can be realized modifying amino acids, because amido bond increase bioavailability therapeutic effect some drugs based on peptide transporter system mammalian which transport peptidyl drugs. derivatives acid, namely acetyl feruloyl tyrosine valyl tyrosine, were synthesized using Fmoc solid-phase...