A5100712420- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Tissue Engineering and Regenerative Medicine
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Data-Driven Disease Surveillance
- Extracellular vesicles in disease
- Renal and related cancers
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- Environmental Impact and Sustainability
- Genetic and phenotypic traits in livestock
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- RNA Research and Splicing
- Radiomics and Machine Learning in Medical Imaging
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Pharmacological Receptor Mechanisms and Effects
- Lipid metabolism and disorders
- Circadian rhythm and melatonin
- Generative Adversarial Networks and Image Synthesis
- Epilepsy research and treatment
- Regional Development and Environment
Xinxiang Medical University
2025
Wenzhou Medical University
2023-2025
Zhejiang Lab
2023-2025
South China Agricultural University
2020-2024
Nanjing Tech University
2022-2024
Kunming University of Science and Technology
2012-2024
Yunnan Yingmao Biotechnology (China)
2022-2024
Macao Polytechnic University
2024
Agricultural Genomics Institute at Shenzhen
2023
Chinese Academy of Agricultural Sciences
2023
The third and fourth weeks of gestation in primates are marked by several developmental milestones, including gastrulation the formation organ primordia. However, our understanding this period is limited due to restricted access vivo embryos. To address gap, we developed an embedded 3D culture system that allows for extended ex utero cynomolgus monkey embryos up 25 days post-fertilization. Morphological, histological, single-cell RNA-sequencing analyses demonstrate cultured largely...
Abstract Single cell approaches have increased our knowledge about the type composition of non-human primate (NHP), but a detailed characterization area-specific regulatory features remains outstanding. We generated single-cell transcriptomic and chromatin accessibility (single-cell ATAC) data 358,237 cells from prefrontal cortex (PFC), primary motor (M1) visual (V1) adult female cynomolgus monkey brain, integrated this dataset with Stereo-seq (spatial enhanced resolution omics-sequencing)...
Given the critical urgency to combat escalating climate crisis and continuous rise in agricultural carbon emissions (ACE) China, accurately forecasting their future trends is crucial. This research employs emission factor method assess ACE throughout mainland China from 1993 2021. To refine our approach, both statistical neural network methodologies were utilized pinpoint key factors influencing ACE. We crafted models incorporating deep learning techniques traditional methods. Notably,...
N1-methyladenosine is a unique type of base methylation in that it blocks Watson-Crick pairing and introduces positive charge. m1A prevalent yeast mammalian mRNA plays functional role. However, little known about the abundance, dynamics, topology this modification plant mRNA. Dot blotting liquid chromatography tandem mass spectrometry analyses revealed dynamic pattern various tissues at different developmental stages petunia (Petunia hybrida), model system for growth development. We...
ABSTRACT Stopping COVID-19 is a priority worldwide. Understanding which cell types are targeted by SARS-CoV-2 virus, whether interspecies differences exist, and how variations in state influence viral entry fundamental for accelerating therapeutic preventative approaches. In this endeavor, we profiled the transcriptome of nine tissues from Macaca fascicularis monkey at single-cell resolution. The distribution facilitators, ACE2 TMRPSS2, different subtypes showed substantial heterogeneity...
Background: Osteosarcoma (OS) is a primary bone tumor associated with locally aggressive growth and early metastatic potential that typically occurs in children adolescents. Chinese traditional medicine Cinnamomum cassia Presl has been shown to have significant tumor-killing effect, which cinnamaldehyde (CA) the main active ingredient. Purpose: To explore anticancer effect of CA on osteosarcoma cells possible molecular mechanism. Methods: Crystal violet assay, MTT assay colony-forming were...
The importance of N6-methyladenine (m6A) in mRNA metabolism, physiology, pathology and other life processes is well recognized. However, the exact role m6A regulators primary Sjögren's syndrome (PSS) remains unclear. In this study, we used bioinformatics machine learning random forest approach to screen eight key from Gene Expression Omnibus GSE7451, GSE40611 GSE84844 datasets. An accurate nomogram model for predicting PSS risk was established based on these regulators. And using consensus...
Background Anxiety disorders have emerged as one of the most prevalent mental health problems and concerns. However, previous research has paid limited attention to measuring public anxiety from a broader perspective. Furthermore, while we know many factors that influence disorders, still an incomplete understanding how these affect anxiety. We aimed quantify perspective Internet searches, analyze its spatiotemporal changing characteristics influencing factors. Methods This study collected...
A novel coronavirus (COVID-19) caused pneumonia broke out at the end of 2019 in Wuhan, China. Many cases were subsequently reported other cities, which has aroused strong reverberations on Internet and social media around world.The aim this study was to investigate reaction global users outbreak COVID-19 by evaluating possibility using monitoring as an instrument handling communicable diseases responding public health emergencies.The disease-related data retrieved from China's National...
It was previously reported that WD repeat domain 5 (Wdr5), a core member of the mammalian Trithorax complex, is key regulator for maintenance embryonic stem (ES) cell pluripotency and somatic reprogramming. However, it remains unclear whether other factors are also involved in this process. Here, we show CTCF an upstream Wdr5: physically associates with Wdr5 further transcriptionally controls its expression by directly targeting gene promoter. As downstream effector, overexpression can...
Domain-domain interactions are a critical type of the mechanisms mediating protein-protein (PPIs). For given protein domain, its ability to combine with distinct domains is usually referred as promiscuity or versatility. Interestingly, previous study has reported that domain's may reflect interact other in human proteins. In this work, promiscuous were first identified from yeast genome. Then, we sought determine what roles might play PPI network. Mapping onto proteins network revealed that,...
Mesenchymal stem cells (MSCs) are one of the most promising adult for clinical application in a cell therapy. The development large-scale cryopreservation techniques, such as vitrification, MSCs is prerequisite therapies. Dimethyl sulfoxide (DMSO) and ethylene glycol (EG) two types cryoprotectants widely used vitrification. However, effects DMSO EG on biological characteristics transcriptome profiles after remain unknown. In present study, viability, immunophenotype surface markers,...
The muscarinic acetylcholine receptor (mAChR) antagonist, scopolamine, has been shown to have a rapid antidepressant effect. And it is believed that GABAergic interneurons play crucial role in this action. Therefore, characterizing the modulation effects of mAChR on for understanding mechanisms underlying scopolamine's effects. In study, we examined effect activation excitatory synaptic transmissions two major subtypes interneurons, somatostatin (SST)- and parvalbumin (PV)-expressing...
Henan Province is located in central China and rich domestic pig populations; Huainan (HN) pigs are one of three indigenous breeds with great performance, including early maturation, strong disease resistance high meat quality. Yunan (YN) black a typical, newly cultivated breed, synthesized between HN American Duroc, subjected to selection for important traits, such as fast growth excellent However, the genomic differences, signatures loci associated economic traits YN still not well...
Macaca fascicularis (M. fascicularis) is a primate model organism that played important role in studying human health. It vital to better understand the similarity and differences of gene regulation between M. human. Current comparative study two species are limited by low quality annotation lack regulatory element data on genome. In this study, we improved with 57 expression from multiple tissues and, more importantly, manual curation procedure. The new enabled us map identify location...
This study aims to assess the potential greenhouse gas (GHG) emissions of delivering 1 kWh from planned offshore wind farm sites grid in Guangdong Province, China. In contrast most previous studies, we avoided underestimating GHG per by approximately 49% adopting a spatialized life-cycle inventory (LCI)-improved stock-driven model under medium scenario combination. We also developed callable LCI spatial differences cells Guangdong. The modeling results indicate that, combination, will range...
Adversarial learning helps generative models translate MRI from source to target sequence when lacking paired samples. However, implementing synthesis with adversarial in clinical settings is challenging due training instability and mode collapse. To address this issue, we leverage intermediate sequences estimate the common latent space among multi-sequence MRI, enabling reconstruction of distinct space. We propose a model that compresses discrete representations each Gaussian distribution...