A5030506735- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Neuroscience and Neural Engineering
- Glycosylation and Glycoproteins Research
- Neuroscience and Neuropharmacology Research
- MicroRNA in disease regulation
- Respiratory viral infections research
- EEG and Brain-Computer Interfaces
- Neuroinflammation and Neurodegeneration Mechanisms
- Muscle activation and electromyography studies
- Molecular Biology Techniques and Applications
- Immune cells in cancer
- Plant tissue culture and regeneration
- Neurological Disease Mechanisms and Treatments
- Extracellular vesicles in disease
- Transgenic Plants and Applications
- Immune Response and Inflammation
- Neuroblastoma Research and Treatments
Nanjing Medical University
2018-2024
Shihezi University
2024
State Key Laboratory of Reproductive Medicine
2024
Abstract Numerous studies have used human pluripotent stem cell-derived cerebral organoids to elucidate the mystery of brain development and model neurological diseases in vitro, but potential for grafted organoid-based therapy vivo remains unknown. Here, we optimized a culturing protocol capable efficiently generating small organoids. After transplantation into mouse medial prefrontal cortex, survived extended projections over 4.5 mm length basal regions within 1 month. The transplanted...
Abstract Human stem cells and derivatives transplantation are widely used to treat nervous system diseases, while the fate determination of transplanted is not well elucidated. To explore cell changes human brain organoids before after transplantation, into prefrontal cortex (PFC) hippocampus (HIP), respectively. Single‐cell sequencing then performed. According time‐series sample comparison, mainly undergo neural development at 2 months post‐transplantation (MPT) glial 4MPT, A different...
Human GABAergic interneurons (GIN) are implicated in normal brain function and numerous mental disorders. However, the generation of functional human GIN subtypes from pluripotent stem cells (hPSCs) has not been established. By expressing LHX6, a transcriptional factor that is critical for development, we induced hPSCs to form GINs, including somatostatin (SST, 29%) parvalbumin (PV, 21%) neurons. Our RNAseq results also confirmed alteration identity with overexpression LHX6. Five months...
GULP1 is an engulfment adaptor protein containing a phosphotyrosine-binding (PTB) domain, and existing studies have shown that it can promote glucose uptake in 3T3-L1 adipocytes. To further explore key metabolically related differential genes downstream of GULP1, this study conducted transcriptome analysis on adipocytes skeletal muscle cells overexpressing GULP1. Subsequently, abnormally expressed were subjected to bioinformatic analysis, real-time fluorescent quantitative PCR (qRT-PCR) was...
Studying tissue composition and function in non-human primates (NHP) is crucial to understand the nature of our own species. Here, we present a large-scale single-cell single-nucleus transcriptomic atlas encompassing over one million cells from 43 tissues adult NHP Macaca fascicularis . This dataset provides vast, carefully annotated, resource study species phylogenetically close humans. As proof principle, have reconstructed cell-cell interaction networks driving Wnt signalling across body,...