A5100660185- Epigenetics and DNA Methylation
- Protist diversity and phylogeny
- RNA modifications and cancer
- Microbial Community Ecology and Physiology
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Circular RNAs in diseases
- Anaerobic Digestion and Biogas Production
- Microbial Metabolic Engineering and Bioproduction
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Biofuel production and bioconversion
- Mosquito-borne diseases and control
- Parasitic Infections and Diagnostics
- Environmental DNA in Biodiversity Studies
- Wnt/β-catenin signaling in development and cancer
- Methane Hydrates and Related Phenomena
- Autophagy in Disease and Therapy
- Thermal properties of materials
- Child Abuse and Trauma
- Chromosomal and Genetic Variations
- Acute Myeloid Leukemia Research
- Phagocytosis and Immune Regulation
Qingdao National Laboratory for Marine Science and Technology
2019-2025
Ocean University of China
2015-2025
Nanjing Medical University
2018-2025
Chinese Academy of Fishery Sciences
2018-2025
Ministry of Agriculture and Rural Affairs
2024-2025
Jiangsu Province Hospital
2025
South China Normal University
2024
Ministry of Education of the People's Republic of China
2024
China Medical University
2016-2024
Fourth Affiliated Hospital of China Medical University
2016-2024
DNA N6-methyladenine (6mA) is newly rediscovered as a potential epigenetic mark across more diverse range of eukaryotes than previously realized. As unicellular model organism, Tetrahymena thermophila among the first reported to contain 6mA modification. However, lack comprehensive information about distribution hinders further investigations into its function and regulatory mechanism. In this study, we provide genome-wide, base pair-resolution map in by applying single-molecule real-time...
Massive tumor-associated macrophage (TAM) infiltration is observed in many tumors, which usually display the immune-suppressive M2-like phenotype but can also be converted to an M1-like antitumor due their high degree of plasticity. The polarization state associated with changes cell shape, morphology activation status. M1 macrophages appeared large and rounded, while M2 were stretched elongated cells. Manipulating has been shown affect macrophages. shape largely dependent on cytoskeletal...
An expanding corpus of research robustly substantiates the complex interrelation between gut microbiota and onset, progression, metastasis colorectal cancer. Investigations in both animal models human subjects have consistently underscored role bacteria a variety metabolic activities, driven by dietary intake. These activities include amino acid metabolism, carbohydrate fermentation, generation regulation bile acids. derivatives, turn, been identified as significant contributors to...
Rediscovered as a potential eukaryotic epigenetic mark, DNA N6-adenine methylation (6mA) varies across species in abundance and its relationships with transcription. Here we characterize AMT1-representing distinct MT-A70 family methyltransferase-in the ciliate Tetrahymena thermophila. AMT1 loss-of-function leads to severe defects growth development. Single Molecule, Real-Time (SMRT) sequencing reveals that is required for bulk of 6mA all symmetric at ApT dinucleotides. The detection...
Background: Liver fibrosis is the consequence of diverse liver injuries and can eventually develop into cirrhosis. Ginkgo biloba extract (GBE) an from dried ginkgo leaves that has many pharmacological effects because its various ingredients been shown to be hepatoprotective. Purpose methods: Aimed investigate underlying protective mechanisms GBE on carbon tetrachloride (CCl 4 )-induced in rats. Male Sprague Dawley rats were randomly divided four groups: control group (C), model (M), low-dose...
WSSV is the deadliest viral pathogen threatening global shrimp aquaculture. The evolutionary history of has remained a mystery, because few relatives, or nimaviruses, had been reported. Our aim was to trace using genomes novel nimaviruses hidden in host genome data. We demonstrate that emerged from diverse family crustacean-infecting large DNA viruses. By comparing and its we show possesses an expanded set unique host-virus interaction-related genes. This extensive gene gain may have key...
Although DNA N 6 -adenine methylation (6mA) is best known in prokaryotes, its presence eukaryotes has recently generated great interest. Biochemical and genetic evidence supports that AMT1, an MT-A70 family methyltransferase (MTase), crucial for 6mA deposition unicellular eukaryotes. Nonetheless, the transmission mechanism remains to be elucidated. Taking advantage of single-molecule real-time circular consensus sequencing (SMRT CCS), here we provide definitive semiconservative Tetrahymena...
Stable inheritance of DNA N 6 -methyladenine (6mA) is crucial for its biological functions in eukaryotes. Here, we identify two distinct methyltransferase (MTase) complexes, both sharing the catalytic subunit AMT1, but featuring AMT6 and AMT7 as their unique components, respectively. While complexes are jointly responsible 6mA maintenance methylation, they exhibit enzymology, DNA/chromatin affinity, genomic distribution, knockout phenotypes. complex, high MTase activity processivity,...
Abstract DNA N6-methyladenine (6mA) is a potential epigenetic mark involved in gene transcription eukaryotes, yet the regulatory mechanism governing its methyltransferase (MTase) activity remains obscure. Here, we exploited 6mA MTase AMT1 to elucidate auto-regulation unicellular eukaryote Tetrahymena thermophila. The detailed endogenous localization of vegetative and sexual stages revealed correlation between reestablishment new MAC occurrence zygotically expressed AMT1. Catalytically...
As the size of transistors shrinks and power density increases, thermal simulation has become an indispensable part device design procedure. However, existing works for advanced technology use simplified empirical models to calculate effective conductivity in simulations. In this work, we present a dataset size-dependent with electron phonon properties extracted from ab initio computations. Absolute in-plane cross-plane data eight semiconducting materials (Si, Ge, GaN, AlN, 4H-SiC, GaAs,...
The zig-zag eel (Mastacembelus armatus) is a unique economic fish species in China and exhibits significant dimorphism of male female phenotypes. Cultivating all-male seedlings can significantly improve production efficiency. To investigate sex differentiation gonadal development M. armatus, high-throughput sequencing technology was used to analyze the transcriptomes gonads at different developmental stages, both before after differentiation. We identified key genes involved differentiation,...
Abstract As the size of transistors shrinks and power density increases, thermal simulation has become an indispensable part device design procedure. However, existing works for advanced technology use simplified empirical models to calculate effective conductivity in simulations. In this work, we present a dataset size-dependent with electron phonon properties extracted from ab initio computations. Absolute in-plane cross-plane data eight semiconducting materials (Si, Ge, GaN, AlN, 4H-SiC,...
The aroma extract of Chickasaw blackberry (Rubus L.) was separated with silica gel normal phase chromatography into six fractions. Gas chromatography−olfactometry (GCO) performed on each fraction to identify active compounds. Aroma extraction dilution analysis (AEDA) employed characterize the profile blackberries from two growing regions United States: Oregon and Arkansas. Comparative AEDA showed that berries grown in had similar compositions; however, those odorants various impacts region....
The lamins are major determinants of nuclear shape and chromatin organization these features frequently altered in prostate cancer (CaP). Human CaP cell lines have lobulations, which enriched A-type but lack B-type been defined as lamin B-deficient microdomains (LDMDs). LDMD frequency is correlated with line aggressiveness increased motility. In addition, LNCaP cells grown the presence dihydrotestosterone (DHT) show an LDMDs. LDMDs activated RNA polymerase II (Pol IIo) androgen receptor (AR)...
A massive amount of somatic mutations has been cataloged in large-scale projects such as The Cancer Genome Atlas (TCGA) and the International Consortium projects. majority found tumor genomes are neutral 'passenger' rather than damaging "driver" mutations. Now, understanding their biological consequences prioritizing them for druggable targets urgently needed. Thanks to rapid advances structural genomics technologies (e.g. X-ray), protein data now made available, providing critical...