A5063217724- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Marine and coastal ecosystems
- Genomics and Phylogenetic Studies
- Marine Biology and Ecology Research
- Parasite Biology and Host Interactions
- Environmental DNA in Biodiversity Studies
- Marine Bivalve and Aquaculture Studies
- Marine Ecology and Invasive Species
- Myxozoan Parasites in Aquatic Species
- Marine and coastal plant biology
- Animal Virus Infections Studies
- Aquatic Invertebrate Ecology and Behavior
- Geology and Paleoclimatology Research
- Aquaculture disease management and microbiota
- Diatoms and Algae Research
- Parasitic Infections and Diagnostics
- Viral Infectious Diseases and Gene Expression in Insects
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Marine and fisheries research
- Physiological and biochemical adaptations
- Marine Toxins and Detection Methods
Ocean University of China
2014-2023
Shandong University
2021-2023
Laoshan Laboratory
2023
Qingdao National Laboratory for Marine Science and Technology
2016-2022
Shaanxi Normal University
2021-2022
Hebei Provincial Center for Disease Control and Prevention
2021
Shanxi Normal University
2020
Google (United States)
2018
Smith College
2014
South China Normal University
2006-2012
Abstract The phylum Ciliophora plays important roles in a wide range of biological studies. However, the evolutionary relationships many groups remain unclear due to lack sufficient molecular data. In this study, dataset was expanded with representatives from 55 orders and all major lineages. main findings are: (1) 14 classes were recovered including one new class, Protocruziea n. cl.; (2) addition two branches, Postciliodesmatophora Intramacronucleata, third branch, Mesodiniea, is...
Abstract The protargol staining method has proved to be indispensable for revealing the cellular structures of a variety protozoa, especially flagellates and ciliates. Protargol provides permanent stains structures: nuclei, extrusomes, basal bodies, microfilamentous constituents cells. Together with older silver nitrate methods, impregnations have provided basis detailed descriptions nearly all ciliates date. performance commercially available preparations varied widely. Recently, suppliers...
Small subunit ribosomal DNA (SSU rDNA) is widely used for phylogenetic inference, barcoding and other taxonomy-based analyses. Recent studies indicate that SSU rDNA of ciliates may have a high level sequence variation within single cell, which impacts the interpretation rDNA-based surveys. However, can come from variety sources including experimental errors, especially mutations generated by polymerase in PCR. In present study, we explore impact four polymerases on find low-fidelity...
Classifications of the Urostyloidea were mainly based on morphology and morphogenesis. Since molecular phylogeny largely focused limited sampling using mostly one-gene information, incongruence between morphological data gene sequences have risen. In this work, three-gene (SSU-rDNA, ITS1-5.8S-ITS2 LSU-rDNA) comprising 12 genera in "core urostyloids" are sequenced, phylogenies these different markers compared maximum-likelihood Bayesian algorithms tested by unconstrained constrained analyses....
A new approach to determining sampling effort for analyzing biofilm-dwelling ciliate colonization was studied in the coastal waters of Yellow Sea, northern China, from May June 2010. The optimal sample size evaluating increased with shortening exposure time, and can be determined according probability recovering those species a specified cumulative contribution communities. More slide-replicates were required at depth 3 m than 1 recover equivalent proportions For routine dynamics analyses,...
Abstract As a model organism for studies of cell and environmental biology, the free‐living cosmopolitan ciliate Euplotes vannus shows intriguing features like dual genome architecture (i.e., separate germline somatic nuclei in each cell/organism), “gene‐sized” chromosomes, stop codon reassignment, programmed ribosomal frameshifting (PRF) strong resistance to stressors. However, molecular mechanisms that account these remarkable traits remain largely unknown. Here we report combined analysis...
Recent advances in high-throughput sequencing and metabarcoding technologies are revolutionizing our understanding of the diversity ecology microbial eukaryotes (protists). The interpretation protist elucidation their ecosystem function are, however, impeded by problems with species delimitation, especially as it applies to molecular taxonomy. Here, using ciliate Euplotes an example, we describe approaches for delimitation based on integrative taxonomy evolutionary ecological perspectives...
The ciliate genus Pleuronema comprises approximately 30 nominal species and has been reported in freshwater, brackish water, marine habitats. Nevertheless, recent studies have indicated that there might be a large undiscovered diversity. In the present work, four new species, namely P. foissneri sp. nov., parasmalli parasalmastra paraorientale collected from Shenzhen, southern China, was investigated using taxonomic methods. diagnosis, description, comparisons with morphologically related...
In order to evaluate the environmental status within a mariculture pond, temporal variations of physico-chemical factors, protist community structure and interactions between biota conditions were investigated during complete cycle in semi-enclosed shrimp-farming waters near Qingdao, north China. Results revealed that: (1) total 54 taxa with ten dominant species was present, comprising 4 chlorophyceans, 2 chrysophyceans, 5 cryptophyceans, 10 dinoflagellates, 3 euglenophyceans, diatoms, 18...
Previous systematic arrangement on the ciliate order Urostylida was mainly based morphological data and only about 20% taxa were analyzed using molecular phylogenetic analyses. In present investigation, 22 newly sequenced species for which alpha-tubulin, SSU rRNA genes or ITS1-5.8S-ITS2 region sampled, refer to all families within order. Following conclusions could be drawn: (1) is not monophyletic, but a core group always present; (2) among family Urostylidae, six of 10 genera are rejected...
The ciliate subclass Haptoria is a diverse taxon that includes most of the free-living predators in class Litostomatea. Phylogenetic study this group was initially conducted using single molecular marker small-subunit ribosomal RNA (SSU rRNA genes). Multi-gene analysis has been limited because very few other sequences were available. We performed phylogenetic analyses incorporating new SSU gene from several debated members taxon, particular, first data Cyclotrichium . also provided nine...