A5032435781- Protist diversity and phylogeny
- Epigenetics and DNA Methylation
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Hedgehog Signaling Pathway Studies
- Cancer-related gene regulation
- Drug Transport and Resistance Mechanisms
- Wastewater Treatment and Nitrogen Removal
- DNA and Nucleic Acid Chemistry
- Amino Acid Enzymes and Metabolism
- Cancer Genomics and Diagnostics
- Environmental DNA in Biodiversity Studies
Ocean University of China
2019-2025
Qingdao National Laboratory for Marine Science and Technology
2019-2025
Although DNA N 6 -adenine methylation (6mA) is best known in prokaryotes, its presence eukaryotes has recently generated great interest. Biochemical and genetic evidence supports that AMT1, an MT-A70 family methyltransferase (MTase), crucial for 6mA deposition unicellular eukaryotes. Nonetheless, the transmission mechanism remains to be elucidated. Taking advantage of single-molecule real-time circular consensus sequencing (SMRT CCS), here we provide definitive semiconservative Tetrahymena...
Stable inheritance of DNA N 6 -methyladenine (6mA) is crucial for its biological functions in eukaryotes. Here, we identify two distinct methyltransferase (MTase) complexes, both sharing the catalytic subunit AMT1, but featuring AMT6 and AMT7 as their unique components, respectively. While complexes are jointly responsible 6mA maintenance methylation, they exhibit enzymology, DNA/chromatin affinity, genomic distribution, knockout phenotypes. complex, high MTase activity processivity,...
Abstract While DNA N 6 -adenine methylation (6mA) is best known in prokaryotes, its presence eukaryotes has generated great interest recently. Biochemical and genetic evidence supports that AMT1, a MT-A70 family methyltransferase (MTase), crucial for 6mA deposition unicellular eukaryotes. Nonetheless, transmission mechanism remains to be elucidated. Taking advantage of Single Molecule Real-Time Circular Consensus Sequencing (SMRT CCS), here we provide definitive semi-conservative 6mA,...
ATP-binding cassette subfamily B (ABCB) has been implicated in various essential functions such as multidrug resistance, auxin transport and heavy metal tolerance animals plants. However, the functions, genomic distribution evolutionary history have not characterized systematically lower eukaryotes. As a lineage of highly specialized unicellular eukaryotes, ciliates extremely diverse features including nuclear dimorphism. To further understand structure this gene family, we investigated ABCB...
Cell synchronization is a powerful tool to understand cell cycle events and its regulatory mechanisms. Counter-flow centrifugal elutriation (CCE) more generally desirable method synchronize cells because it does not significantly alter behavior and/or progression, however, adjusting specific parameters in type/equipment-dependent manner can be challenging. In this paper, we used the unicellular eukaryotic model organism, Tetrahymena thermophila as testing system for optimizing CCE workflow....
Abstract Stable inheritance of DNA N 6 -methyladenine (6mA) is crucial for its biological functions in eukaryotes. Here, we identify two distinct methyltransferase (MTase) complexes, both sharing the catalytic subunit AMT1, but featuring AMT6 and AMT7 as their unique components, respectively. While complexes are jointly responsible 6mA maintenance methylation, they exhibit enzymology, DNA/chromatin affinity, genomic distribution, knockout phenotypes. complex, high MTase activity...
Abstract Pseudocohnilembus persalinus is a free‐living marine scuticociliate that, as new model organism, has been used in wide variety of studies. However, long‐term laboratory maintenance for this species mainly achieved by subculture that requires rigorous culture environments and, too often, cultures the organism die out reasons. Successful transport viable also poses problems researchers. This study describes simple and rapid protocol cryopreservation P. . The effects physiological...