A5078036044- Estrogen and related hormone effects
- RNA regulation and disease
- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- interferon and immune responses
- Retinoids in leukemia and cellular processes
- Cholesterol and Lipid Metabolism
- Metabolism, Diabetes, and Cancer
- RNA Research and Splicing
- Adipokines, Inflammation, and Metabolic Diseases
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Drug Transport and Resistance Mechanisms
- Retinal Imaging and Analysis
- Bone Metabolism and Diseases
- Natural product bioactivities and synthesis
- Retinal Diseases and Treatments
- Computational Drug Discovery Methods
- Liver Disease Diagnosis and Treatment
- Andrographolide Research and Applications
- Protein Kinase Regulation and GTPase Signaling
University of Alberta
1992-2023
Chinese University of Hong Kong
2015-2022
Bioscience (China)
2018-2020
Chinese Academy of Sciences
2006-2017
Queen Mary Hospital
2017
University of Hong Kong
2017
Shanghai Institute of Materia Medica
2017
University of Connecticut
2009-2016
Guangzhou Institutes of Biomedicine and Health
2006-2011
University of Science and Technology of China
2008-2010
Adiponectin protects the vascular system partly through stimulation of endothelial nitric oxide (NO) production and endothelium-dependent vasodilation. The current study investigated role two recently identified adiponectin receptors, AdipoR1 -R2, their downstream effectors in mediating endothelium actions adiponectin. In human umbilical vein cells, adiponectin-induced phosphorylation NO synthase (eNOS) at Ser(1177) were abrogated when expression -R2 simultaneously suppressed. Proteomic...
Numerous studies have demonstrated that estrogens induce rapid and transient activation of the Src/Erk phosphorylation cascade. Activation this cascade triggers vital cellular functions including cell proliferation differentiation. However, details molecular mechanism process remain to be elucidated. We identified a previously uncharacterized nuclear receptor-interacting protein designated as modulator nongenomic activity estrogen receptor (MNAR). Here we show MNAR modulates...
Vitamin E is comprised of two classes compounds: tocopherols and tocotrienols. Tocotrienol-enriched palm oil has been shown to help reduce blood glucose levels in patients preclinical animal models. However, the mechanistic basis for tocotrienol action not well established. Peroxisome proliferator-activated receptors alpha, gamma, delta (PPARalpha, PPARgamma, PPARdelta) are ligand-regulated transcription factors that play essential roles energy metabolism. Importantly, synthetic PPARalpha...
MicroRNA-153 (miR-153) is a brain-specific miRNA that expressed at significantly lower level in glioblastoma (GBM) relative to non-neoplastic brain tissue. Although the expression pattern of miR-153 has been extensively established, its target genes and cellular mechanism remain undefined. To investigate into potential function glioblastmas, we transfected GBM cell line DBTRG-05MG with synthetic oligos observed decreased proliferation increased apoptosis. Bioinformatics analysis revealed...
MicroRNAs (miRNAs) are one class of short, endogenous RNAs which can regulate gene expression at the post-transcriptional level. Previous analysis revealed that mammalian miRNAs tend to cluster on chromosomes. However, functional consequences this clustering and conservation property largely unknown. In study we present a method identify signaling pathways targeted by clustered miRNAs. We performed computational screen for mouse miRNA clusters. Here, report target genes 3 clusters...
Rationale: Men and postmenopausal women are more prone to developing non-alcoholic fatty liver disease/steatohepatitis (NAFLD/NASH) than premenopausal women. However, the pathological links underlying mechanisms of this disparity still elusive. The sex-difference in hepatic very low-density lipoprotein (VLDL) assembly secretion may contribute NAFLD development. Estrogen-related receptor alpha (ERRα) is a key regulator several metabolic processes. We hypothesized that ERRα plays role...
Abstract Excessive bone marrow adipocytes (BMAds) accumulation often occurs under diverse pathophysiological conditions associated with deterioration. Estrogen-related receptor α (ESRRA) is a key regulator responding to metabolic stress. Here, we show that adipocyte-specific ESRRA deficiency preserves osteogenesis and vascular formation in adipocyte-rich upon estrogen or obesity. Mechanistically, adipocyte interferes E2/ESR1 signaling resulting transcriptional repression of secreted...
Analysis of estrogen receptor α and β interplay with other transcription factors is critical to the understanding how small molecules, cognate ligands for these receptors, selectively regulate mode amplitude gene by affecting activity. To better understand molecular mechanisms selective action ligands, we characterized (ER) interaction p160 family coactivators. We also investigated interactions are affected binding specific ligands. show that ER utilize different LXXLL motifs their members....
The Na+/H+ exchanger is a pH-regulatory protein that extrudes one H+ ion in exchange for Na+ when intracellular pH declines. A number of studies have shown phorbol ester stimulation activity intact cells, leading to the idea regulated by kinase C-mediated phosphorylation vivo. cDNA encoding has been cloned, and recent model suggests large internal cytoplasmic C-terminal domain may be site regulation [Sardet, Franchi & Pouyssegur (1989) Cell 56, 271-280]. We examined this region using...
PGC‐1 alpha (uniprotkb: Q9UBK2 ) and ERR gamma P62508 bind (MI: 0407 by surface plasmon resonance 0107
Hepatocyte nuclear factor-4 alpha (HNF-4α) is an important transcription factor governing the expression of genes involved in multiple metabolic pathways. Secreted phospholipase A(2) GXIIB (PLA(2) GXIIB) atypical member a class secreted phospholipases . We establish this study that PLA(2) HNF-4α target gene. demonstrate binds to response element on promoter. Moreover, agonists induce human hepatocarcinoma cells. Importantly, GXIIB-null mice accumulate triglyceride, cholesterol, and fatty...
Nuclear receptors regulate transcription in direct response to their cognate hormonal ligands. Ligand binding leads the dissociation of corepressors and recruitment coactivators. Many these factors, acting large complexes, have emerged as potential chromatin remodelers through intrinsic histone modifying activities. In addition, other ligand-recruited complexes appear act more directly on transcriptional apparatus. The DRIP complex is a 15-subunit required for nuclear receptor activation...
MicroRNAs (miRNAs) are noncoding RNAs that regulate gene expression by inhibiting translation or promoting mRNA degradation. Previously, we established microRNA-153 (miR-153) induces apoptosis downregulating B-cell lymphoma 2 (Bcl-2) and myeloid cell leukemia sequence 1 (Mcl-1) protein levels in glioblastoma line DBTRG-05MG. In our study, show ectopic of miR-153 also inhibits the kinase B (PKB/Akt) pathway via reducing level insulin receptor substrate-2 (Irs-2). Moreover, simultaneous...
MINT‐7297297, MINT‐7297435: PGC‐1 alpha NR2 (uniprotkb:Q9UBK2) binds (MI:0407) to ERR gamma (uniprotkb:P62508) by surface plasmon resonance (MI:0107)
Abstract Objective: Although oestrogen‐related receptor α (ERRα) is primarily thought to regulate energy homeostasis, it also serves as a prognostic marker for cancer. The aim of this study was investigate any connection between ERRα activity and cell population growth. Materials methods: XCT‐790, an ERRa specific inverse agonist, employed suppress in human non‐small lung cancer cells (NSCLC) A549. Gene expressions were detected using quantitative real‐time PCR Western blot analysis....
Weighted gene co-expression network analysis (WGCNA) identified a cell-cycle module that is associated with poor prognosis and aggressiveness of glioma. One the core members, Regulator chromatin condensation 2 (RCC2) component chromosome passenger complex. Accumulating evidence suggests RCC2 plays vital role in mitotic process abnormal expression involved cancer development. Gene silencing experiments show required for glioma cell proliferation migration. RNA-Sequencing reveals dual both...
Nuclear hormone receptors are hormone-regulated transcription factors that bind to specific sites on DNA and modulate the expression of adjacent target genes. Many nuclear display bimodal transcriptional properties; thyroid receptors, for example, typically repress gene in absence hormone, but activate presence hormone. The ability is closely linked apo-receptor physically auxiliary corepressor proteins denoted SMRT (silencing mediator retinoic acid receptor) N-CoR (nuclear receptor...
Abstract Oleanolic acid (OA) is an ingredient found in some Traditional Chinese Medicine remedies for treating liver ailments. Bile biosynthesis and catabolism are part controlled the by transcription factor farnesoid X receptor (FXR). It was hypothesized that OA may act through FXR to mediate of its beneficial health effects. In this study, it bound ligand binding domain (LBD) blocked ability interact with coactivator SRC‐3. also dose‐dependently suppressed activity FXR‐LBD induced...