A5080813523- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Cardiomyopathy and Myosin Studies
- Allergic Rhinitis and Sensitization
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Dermatology and Skin Diseases
- SARS-CoV-2 and COVID-19 Research
- Tuberculosis Research and Epidemiology
- Asthma and respiratory diseases
- Cancer-related molecular mechanisms research
- Mycobacterium research and diagnosis
- Hepatitis B Virus Studies
- Cancer-related gene regulation
- Machine Learning in Bioinformatics
- Bacteriophages and microbial interactions
- Food Allergy and Anaphylaxis Research
- Genetic Associations and Epidemiology
- Bioinformatics and Genomic Networks
- MicroRNA in disease regulation
- HIV Research and Treatment
- Hepatitis C virus research
- Bone Tumor Diagnosis and Treatments
Chinese University of Hong Kong
2016-2025
University of Hong Kong
2009-2023
First People's Hospital of Foshan
2023
SickKids Foundation
2022
Hospital for Sick Children
2022
Norwegian Womens Public Health Association
2017-2020
Weatherford College
2020
Tung Wah Eastern Hospital
2015
Prince of Wales Hospital
2003-2014
University of California System
2013
Circular RNAs are a class of regulatory RNA transcripts, which ubiquitously expressed in eukaryotes. In the current study, we evaluate function novel circRNA derived from β-catenin gene locus, circβ-catenin. Circβ-catenin is predominantly localized cytoplasm and displays resistance to RNase-R treatment. We find that circβ-catenin highly liver cancer tissues when compared adjacent normal tissues. Silencing significantly suppresses malignant phenotypes vitro vivo, knockdown this reduces...
Rationale: Malignant ascites in peritoneal metastases is a lipid-enriched microenvironment and frequently involved the poor prognosis of epithelial ovarian cancer (EOC). However, detailed mechanisms underlying (OvCa) cells dictating their lipid metabolic activities promoting tumor progression remain elusive. Methods: The omental conditioned medium (OCM) was established to imitate or microenvironment. Mass spectrometry, RT-qPCR, IHC, western blot assays were applied evaluate human fatty acid...
Abstract Background Metastatic colonization is one of the critical steps in tumor metastasis. A pre-metastatic niche required for metastatic and determined by tumor-stroma interactions, yet mechanistic underpinnings remain incompletely understood. Methods PCR-based miRNome profiling, qPCR, immunofluorescent analyses evaluated expression exosomal miR-141 cell-to-cell communication. LC-MS/MS proteomic profiling Dual-Luciferase identified YAP1 as direct target miR-141. Human cytokine ChIP,...
Previously, we identified a new mammalian sHSP, MKBP, as myotonic dystrophy protein kinase-binding protein, and suggested its important role in muscle maintenance (Suzuki, A., Sugiyama, Y., Hayashi, Nyu-i, N., Yoshida, M., Nonaka, I., Ishiura, S., Arahata, K., Ohno, S. (1998) J. Cell Biol. 140, 1113-1124). In this paper, develop the former work by performing extensive characterization of five six sHSPs so far identified, that is, HSP27, alphaB-crystallin, p20, MKBP/HSPB2, HSPB3, omitting...
A sequenced house dust mite (HDM) genome would advance our understanding of HDM allergens, a common cause human allergies.We sought to produce an annotated Dermatophagoides farinae draft and develop combined genomic-transcriptomic-proteomic approach for elucidation allergens.A D transcriptome were assembled with high-throughput sequencing, accommodating microbiome sequences. The allergen gene structures validated by means Sanger sequencing. mite's composition was determined, the predominant...
Abstract Background DNA methylation is an important type of epigenetic modification involved in gene regulation. Although strong at promoters widely recognized to be associated with transcriptional repression, many aspects remain not fully understood, including the quantitative relationships between and expression levels, individual roles promoter body methylation. Results Here we present integrated analysis whole-genome bisulfite sequencing RNA data from human samples cell lines. We find...
Diabetes and obesity are complex diseases associated with insulin resistance fatty liver. The latter is characterized by dysregulation of the Akt, AMP-activated protein kinase (AMPK), IGF-I pathways expression microRNAs (miRNAs). In China, multicomponent traditional Chinese medicine (TCM) has been used to treat diabetes for centuries. this study, we a three-herb, berberine-containing TCM male Zucker diabetic rats. showed sustained glucose-lowering effects 1 week after single-dose treatment....
Panax notoginseng (Burk.) F.H. Chen is one of the most highly valued medicinal plants in world. The major bioactive molecules are triterpene saponins, which also known as ginsenosides. However, its large genome size has hindered assembly a draft by whole sequencing. Hence, genomic and transcriptomic details about P. notoginseng, especially biosynthetic pathways gene expression different parts plant, have remained largely unknown until now. In this study, RNA sequencing three tissues was...
The pandemic novel coronavirus infection, Coronavirus Disease 2019 (COVID-19), has affected at least 190 countries or territories, with 465,915 confirmed cases and 21,031 deaths. In a containment-based strategy, rapid, sensitive specific testing is important in epidemiological control clinical management. Using 96 SARS-CoV-2 104 non-SARS-CoV-2 genomes our in-house program, GolayMetaMiner, four regions longer than 50 nucleotides the genome were identified. Primers designed to target longest...
OBJECTIVE Several studies support associations between relative leukocyte telomere length (rLTL), a biomarker of biological aging and type 2 diabetes. This study investigates the relationship rLTL risk glycemic progression in patients with RESEARCH DESIGN AND METHODS In this cohort study, consecutive Chinese diabetes (N = 5,506) from Hong Kong Diabetes Register stored baseline DNA available follow-up data were studied. was measured using quantitative PCR. Glycemic defined as new need for...
Objective: RAC1 aberrations in head and neck squamous cell carcinoma (HNSCC) remain clinically inactionable today. Methods: Here, we investigated the clinical significance potential druggability of genomic HNSCC. Results: Notably, HPV(−)HNSCC patients bearing unique HNSCC-prevalent RAC1-A159V hotspot mutation, P29S G-box domain mutations, copy number increases all displayed dismal overall survival (TCGA-HNSCC). demonstrated that five HNSCC patient-relevant tested (A159V K116N, G15S, N39S)...
Abstract Background The Hong Kong orchid tree Bauhinia × blakeana Dunn has long been proposed to be a sterile interspecific hybrid exhibiting flower heterosis when compared its likely parental species, purpurea L. and variegata Here, we report comparative genomic transcriptomic analyses of the 3 species. Findings We generated chromosome-level assemblies for species applied trio-binning approach construct haplotype-resolved telomere-to-telomere (T2T) genome B. blakeana. Comparative...
Large-scale partial sequencing of cDNA libraries to generate expressed sequence tags (ESTs) is an effective means discovering novel genes and characterizing transcription patterns in different tissues. To catalogue the identities expression levels cardiovascular system, we initiated large-scale analysis human cardiac libraries.Using automated DNA sequencing, generated 43,285 ESTs from heart libraries. An additional 41,619 were retrieved public databases, for a total 84,904 representing more...
An outbreak of severe acute respiratory syndrome (SARS) occurred in China and the first case emerged mid-November 2002. The aetiological agent this disease was found to be a previously unknown coronavirus, SARS-associated coronavirus (SARS-CoV). detailed pathology SARS-CoV infection host response viral are still not known. 3a gene encodes non-structural protein, which is predicted transmembrane protein. In study, it shown that protein expressed lungs intestinal tissues SARS patients...
Ninety-six percent of rifampicin resistance in Mycobacterium tuberculosis was shown to be associated with mutations inside the 81 bp resistance-determining region (RRDR) located centre rpoB gene. The detection by targeting RRDR failed match a resistant phenotype 4% all cases. Our study aims identify outside that are M. tuberculosis. Among 50 rifampicin-resistant and 20 rifampicin-susceptible clinical isolates tuberculosis, 2 did not harbour any known RRDR. Sequencing analysis whole gene...
Recent studies revealed that the herb <i>Andrographis paniculata</i> possesses cardioprotective activities. Using neonatal rat cardiomyocytes, actions of several diterpene lactones derived from <i>A. including andrographolide, 14-deoxyandrographolide, 14-deoxy-11,12-didehydroandrographolide, and sodium 14-deoxyandrographolide-12-sulfonate were investigated. Pretreatment with andrographolide but not other compounds protected cardiomyocytes against hypoxia/ reoxygenation injury up-regulated...