A5045145986- Cancer Genomics and Diagnostics
- Animal Genetics and Reproduction
- RNA modifications and cancer
- interferon and immune responses
- Amoebic Infections and Treatments
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Sarcoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Molecular Biology Techniques and Applications
- Peptidase Inhibition and Analysis
- Cancer Cells and Metastasis
- NF-κB Signaling Pathways
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- Uterine Myomas and Treatments
- Silk-based biomaterials and applications
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Neuropeptides and Animal Physiology
- Parasitic Infections and Diagnostics
The University of Texas MD Anderson Cancer Center
2010-2024
Houston Methodist
2013-2023
Universidad Autónoma de Nuevo León
2005-2022
Universidad Nacional Autónoma de México
2020
Universidad Tecnológica Emiliano Zapata del Estado de Morelos
2014
Technological University of Mexico
2014
University of Carabobo
2007
Spanish Oncology Genitourinary Group
2007
University of Utah
2003
Abstract TWEAK (TNF-like weak inducer of apoptosis) is a TNF superfamily member implicated in several mechanisms. Although fibroblast growth factor inducible 14 (Fn14)/TweakR has been reported as its receptor, an yet unrecognized surface molecule(s) might modulate function(s). Thus, we set out to identify TWEAK-binding proteins by screening combinatorial peptide library. Cyclic peptides containing consensus motif (WXDDG) bound specifically. These were similar CD163, scavenger receptor...
Processes that promote cancer progression such as angiogenesis require a functional interplay between malignant and nonmalignant cells in the tumor microenvironment. The metalloprotease aminopeptidase N (APN; CD13) is often overexpressed has been implicated progression. Our previous studies of APN-null mice revealed impaired neoangiogenesis model systems without suggested hypothesis APN expressed by might growth. We tested this comparing effects deficiency allografted (tumor) (host) on...
Significance The poor prognosis of epithelial ovarian cancer (EOC) has not improved for several decades because drug resistance to current anticancer drugs. Furthermore, few molecularly targeted agents are effective EOC, likely EOC high tumor heterogeneity. Discovering new targets and mechanisms involved in the progression is therefore sorely needed. Our multiple CRISPR RNAi-based vivo loss-of-function screens have identified candidate targets, including druggable oncogene KPNB1, whose...
Aminopeptidase N (APN, CD13; EC 3.4.11.2) is a transmembrane metalloprotease with several functions, depending on the cell type and tissue environment. In tumor vasculature, APN overexpressed in endothelium promotes angiogenesis. However, there have been no reports of vivo inactivation gene to validate these findings. Here we evaluated, by targeted disruption gene, whether participates blood vessel formation function under normal conditions. Surprisingly, APN-null mice developed gross or...
Significance Epithelial-mesenchymal transition (EMT) contributes to metastasis and chemoresistance in patients with hepatocellular carcinoma (HCC), but the genes driving EMT are poorly understood. Here, we describe a transposon mutagenesis screen that made it possible identify 233 candidate cancer (CCGs) enriched for HCC. Twenty-three CCGs predicted function early tumorigenesis, alterations these associated poor HCC patient survival. Validation studies showed deregulation of most highly...
Significance Triple-negative breast cancer (TNBC) is the most aggressive subtype. Despite extensive genome-sequencing efforts, there still an incomplete understanding of genetic networks driving TNBC. Here, we used Sleeping Beauty transposon mutagenesis to identify genes that cooperate with mutant P ten in induction We identified 12 candidate TNBC trunk drivers and a larger number progression genes. Subsequent functional validation studies eight human tumor suppressor genes, including...
Abstract The critical role of the tumor immune microenvironment (TIME) in determining response to checkpoint inhibitor (ICI) therapy underscores importance understanding cancer cell–intrinsic mechanisms driving immune-excluded (“cold”) TIMEs. One such cold is oral cavity squamous cell carcinoma (OSCC), a tobacco-associated with mutations TP53 gene which responds poorly ICI therapy. Because altered function promotes progression and plays potential TIME modulation, here we developed syngeneic...
Objectives/Goals: Clinical relevance of preclinical animal models is commonly in question. Herein, we investigated locoregional tumor immune microenvironment (TIME) differences tumor-bearing murine oral cancer models, unresponsive to traditional immunotherapy, and also developed an resection model ultimately enhance translational relevance. Methods/Study Population: Here, utilized carcinogen-induced, HPV-negative models. For TIME studies, ROC1 cells were maintained as published. tumors...
Cytoskeletal-associated proteins play an active role in coordinating the adhesion and migration machinery cancer progression. To identify functional protein networks potential inhibitors, we screened internalizing phage (iPhage) display library tumor cells, selected LGRFYAASG as a cytosol-targeting peptide. By affinity purification mass spectrometry, intracellular annexin A2 was identified corresponding binding protein. Consistently, cell-internalizing, penetratin-fused version of peptide...
Phage display screening allows the study of functional protein–protein interactions at cell surface, but investigating intracellular organelles remains a challenge. Here we introduce internalizing-phage libraries to identify clones that enter mammalian cells through receptor-independent mechanism and target-specific as tool select ligand peptides their receptors. We demonstrate penetratin, an antennapedia-derived peptide, can be displayed on phage envelope mediate uptake internalizing into...
Significance The progression of many solid tumors is associated with increased vascularization. We previously recognized involvement in tumor development and angiogenesis stromal cells expressing the CD13 protease aminopeptidase. basic biological concept participation nontumor cancer stroma microenvironment strengthened present study by our finding that a CD11b + myeloid subset bone marrow-derived affects pericyte biology thereby influences growth metastasis. Therapeutic implications...
Background TP53 , the most mutated gene in solid cancers, has a profound impact on hallmarks of cancer. Somatic mutations occur high frequencies head and neck including oral squamous cell carcinoma (OSCC). Our study aims to understand role gain-of-function mutation modulating tumor immune microenvironment (TIME) OSCC. Methods Short hairpin RNA knockdown mutant p53R172H syngeneic tumors demonstrated changes growth between immunocompetent immunodeficient mice. HTG EdgeSeq targeted messenger...
Hydrogels have become a promising research focus because of their potential for biomedical application. Here we explore the long-range, electrostatic interactions by following effect trans-acting (pH) and cis-acting factors (peptide mutation) on formation Au-phage hydrogels. These bioinorganic hydrogels can be generated from bottom-up assembly Au nanoparticles (Au NP) with either native or mutant bacteriophage (phage) through interaction phage pVIII major capsid proteins (pVIII). The factor...
Abstract Although genomic sequencing has provided a better understating of the genetic landmarks in triple-negative breast cancer (TNBC), functional validation candidate genes (CCG) remains unsolved. In this study, we used transposon mutagenesis strategy based on two-step sleeping beauty (SB) forward screen to identify and validate new tumor suppressors (TS) disease. We generated 120 siRNAs targeting 40 SB-identified TS them downregulate expression these four human TNBC cell lines. Among...